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Patología Revista Latinoamericana

2009, Number 2

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Patol Rev Latinoam 2009; 47 (2)

Gestational trophoblastic disease: histopathologic classification and immnunoshistochemical staining properties pf p53, Β-hCG, PLAP and vimentine

Valverde D

Language: Spanish
References: 42
Page: 96-102
PDF size: 296.02 Kb.


ABSTRACT

Background: The gestational trophoblastic disease is a group of benign and malignant conditions: Hydatidiform moles result from abnormal fertilization and have been divided into partial, complete and invasive mole based on morphologic, cytogenetic, and clinical features. Another group are the neoplasms of trophoblast (choriocarcinoma, exaggerated placental site and others). Hydatidiform mole is the most frequent form and it is manifested during the first trimester of pregnancy, generally as spontaneous abortion.
Objective: To study the immunohistochemical expression of some markers leading have a better correlation with histological parameters identified with the conventional routine technique.
Material and methods: Thirty-three cases with diagnosis of trophoblastic diseases from pathological anatomy department, Hospital Escuela Oscar Danilo Rosales, were studied, as well as 33 cases of products of uterine curettage without trophoblastic disease.
Results: p53 immunoreactivity was stronger in complete mole (++) and choriocarcinoma (+++) than in normal placenta and partial mole. PLAP was expressed in the apical and basal portion of the plasmatic membrane of sincytiotrophoblast cells. β-hCG was expressed in decidua, endometrial tissue and compact layer glands.
Conclusions: Pathology predominant diagnosis was of complete mole, with classic characteristics due to its diagnosis during the second trimester of pregnancy. Vimentin immunohistochemical expression was present in all mesenchymal components of tissues corresponding to cases and controls. p53 immunostaining was more intense (+++) in cases with choriocarcinoma diagnosis. Β-hCG was expressed with intensity (+++) in cases of complete mole and choriocarcinoma, specially at the cytoplasm of sincytiotrophoblast cells.


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