Isolates in blood-cultures ten years follow-up in a pediatric hospital

Blanca Leaños Miranda; Martha Abad Acosta; Fortino Solórzano Santos; Ma Guadalupe Miranda Novales

2007, Number 1

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Enf Infec Microbiol 2007; 27 (1)

Isolates in blood-cultures ten years follow-up in a pediatric hospital

Leaños MB, Abad AM, Solórzano SF, Miranda NMG

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Language: Spanish
References: 16
Page: 6-10
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ABSTRACT

Objective. To inform the frequency of isolation of microorganisms in blood cultures during a 10 year period in the Hospital de Pediatría (HP) CMN SXXI.
Material and methods. All blood cultures were processed in the Microbiology section of the clinical laboratory. A blind subculture and smear was done in the first 24 h. Those with suspicion or positive for growth were subculture in blood, chocolate and Mac Conkey agar. The bottles were incubated at 35-37 °C and observed during 10 days. Those with diagnosis of mycotic infection or brucellosis were conserved for 21 days. At 10th day, a blind subculture was done in blood agar and discarded if negative. The growth in agar plates was identified according to conventional biochemical tests. For the analysis we used descriptive statistics.
Results. A total of 26,535 blood cultures obtained from hospitalized patients were reviewed. Frequency of positive cultures varied from 17% to 21.9%. The microorganisms isolated more frequently were coagulase-negative Staphylococcus (CNS), Staphylcoccus aureus, Klebsiella pneumoniae, Escherichia coli and Enterobacter spp. Recuperation of Streptococcus pneumoniae and Salmonella spp. decreased and Candida spp. increased from 3.5 to 10%. An outbreak due to Serratia marcescens ocurred in 1995.
Conclusions. Frequency of positive blood cultures was similar to different reports in the literature. CNS had maintained the first place during a decade.

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