Hernández SF, Marichi RFJ, Álvarez MA

Language: Spanish
References: 25
Page: 21-26
PDF size: 274.30 Kb.

ABSTRACT

Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum. BSP was originally isolated from bovine cortical bone as a 23-kDa glycopeptide but the native BSP has an apparent molecular weight of 60-80 kDa based on SDS-PAGE, which is a considerable deviation from the predicted weight (based on cDNA sequence) of approximately 33 kDa. The protein is highly acidic (pKa of ~ 3.9) and contains a large amount of Glu residues, constituting ~22% of the total aminoacid. This suggests that the protein have several spatially-segmented functional domains including a hydrophobic collagen-binding domain, a hydroxyapatite-nucleating region of contiguous glutamic acid residues and a classical integrin-binding motif (RGD) near the C-terminal. This protein plays a regulatory role in the mineralization and growth of mineral hard tissues. New formation of mineral hard tissue are key role in orthodontic tooth movements characterized by applied mechanical forces that cause tension in the cells which induce a cellular adaptation and this adaptation can activate cellular and molecular responses, that affect the extracellular matrix of periodontal tissues. For this, the aim of this study was to evaluate Bone Sialoprotein expression associated to remodeling of the periodontium after applied orthodontic forces. The upper and lower first bicuspid were applied with braces 0.022 Roth System with 0.016 Nickel Titanium archwires, in all teeth, except the left upper and lower first bicuspid. The first bicuspid without braces (t = 0) and with braces for inducing orthodontic tooth movements during 1, 3, 5, 7 and 9 days; were extracted for analyzing the Bone Sialoprotein expression in the extracellular matrix of the periodontal ligament area. For evaluating the temporal and spatial expression of RNA messengers of Bone Sialoprotein in periodontal ligament the RT-PCR technique was carried out. The expression of Bone Sialoprotein in the experimental group was present at 1, 3 and 9 days and absent at 5 and 7 days of experimental test, suggesting that orthodontic tooth movements will produce significant changes and these changes will be susceptible in the concentrations of the mRNA of Bone Sialoprotein.

REFERENCES

1. Hassel T. Tissues and cells of the periodontum. Periodontologi 2000; 3: 9-38.

2. Quian H et al. The influence of PDL principal fibers in a 3 - dimensional analysis of orthodontic tooth movement. Am J Orthod Dentofacial Orthop September 2001; 120 (3): 272-279W.

3. Garant PR. Oral cells and tissues. Editorial Quintessence Canada 2003.

4. Proffit. Ortodoncia contemporánea. 3ª Edición. Ed. Harcourt. España 2001.

5. Norton LA. The biology of tooth movement. Editorial CRC Press. Florida 1989.

6. Grieve WG. Prostaglandin E (PGE) and interleukin- 1 (IL-1) levels in gingival crevicular fluid during human orthodontic tooth movement. Am J Orthod Dentofac Orthop 1994; 105: 369-74.

7. Abbas HM. Leukotrienes in orthodontic tooth movement. Am J Orthod Dentofac Orthop 1989; 95: 321-329.

8. Mayumi S, Shigeru S. Interleukina 1 beta and prostaglandin E are involved in the response of periodontal cells to mechanical stress in vivo and in vitro. Am J Orthod Dentofac Orthop 1991; 99: 226-40.

9. Najat A, Lars F. Orthodontic tooth movement and de novo synthesis of proinflammatory cytokines. Am J Orthod Dentofac Orthop 2001; 119: 307-12.

10. Davidovitch Z, Shanfeld L. Cyclic AMP in alveolar bones of orthodontically- treated cats. Arch Oral Biol 1975; 20: 567-574.

11. Kui FM. Study of the effects of PGE CH! On orthodontic tooth movement. J Jpn Orthod Soc 1992; 51: 148-152.

12. Collins MK. The local use of vitamin D t increase the rate of orthodontic tooth movement. Am J Orthod Dentofac Orthop 1988; 94: 278-284.

13. Bumman A et al. Collagen synthesis from human PDL cells following orthodontic tooth movement. Eur J Orthod 1997 Feb; 19(1): 29-37.

14. Meir R et al. Expression of trophoelastin in human periodontal ligament fibroblasts after stimulation of orthodontic force. Archives of Oral Biology 2004; 49: 119-124.

15. Deguchi T et al. Galanin-immunoreactive nerve fibers in the periodontal ligament during experimental tooth movement. J Dent 2003; 82 (9): 677-681.

16. Reyna J, Grageda E et al. Gene expression induced by orthodontic tooth movement and/or root resorption. Biological mechanisms of tooth eruption and movement. Edited by Davidovitch 2006.

17. Ong MMA. Periodontic and orthodontic treatment in adults. Am J Orthod Dentofac Orthop 2002; 122: 420-428.

18. Uematsu S, Mogi M. Interleukin (IL-1 IL-6), tumor necrosis factor-alfa, epidermal growth factor, and microglobulin levels are elevated in gingival crevicular fluid during human orthodonthic tooth movement. J Dent Res 1996; 75 (1): 562-567.

19. Keigo H. Involvement of nitric oxide in orthodontic tooth movement in rats. Am J Orthod Dentofac Orthop 2002; 112: 306-9.

20. Holliday LS, Vakani A. Effects of matrix metalloproteinase inhibitors on bone resorption and orthodontic tooth movement. J Dent Res 2003; 82 (9): 687-691.

21. Magdalena CM. c-fos expression in rat brain nuclei following incisor tooth movement. J Dent Res 2004; 83 (1): 50-54.

22. Ganss B, Kim RH. Bone sialoprotein. Crit Rev Oral Biol Med 1999; 10 (1): 79-98.

23. Domon S, Shimokawa H. Temporal and spatial mRNA expression of bone sialoprotein and type I collagen during rodent tooth movement. European Journal of Orthodontics 2001; 23: 339-348.

24. Spurrier SW. A comparison of apical root resorption during orthodontic treatment in endodontically treated and vital teeth. Am J Orthod Dentofac Orthop 1190; 97: 130-4.

25. Jerome J, Grageda E et al. Celebrex offer a small protection from root resorption associated with orthodontic movement. CDA Journal Vol. 33 No. 12 Diciembre 2005.