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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

1999, Number 2

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Rev Mex Patol Clin Med Lab 1999; 46 (2)

Cytological detection of human papillomavirus and its correlation with PCR: A prospective study of 55 cases

Curiel VJJ, Lozada MOR, Berumen J, Briones J, Catarino A

Language: Spanish
References: 21
Page: 74-78
PDF size: 81.15 Kb.


ABSTRACT

Human papillomavirus (HPV) disease is traditionally detected by cytological study of uterine cervix (Papanicolaou) and recently by viral sequence detection with several methods of molecular biology. This disease is clinically detected by colposcopy. There are very rigorous criteria to diagnose cytologically HPV infection in one cell, with 20 to 40% of false negative. In our laboratory, we have detected patients with this disease by means of cytological study with more subtle criteria; some of this changes are known as pseudokoilocytic ones. In 55 patients, in who subtle changes were found, mainly cytoplasmic membrane reinforcement, detection of the virus by molecular biology with PCR was carried out. We found in 33 of them (60%) the presence of virus was positive and in addition, 10 more cases we found by colposcopy the corroboration of virus infection. Whit basis on the above mentioned, we conclude the existence of these more subtle criteria which can be used for HPV detection, with correlation of 78.2% with molecular biology and colposcopy together, with a sensibility of 100% and specificity of 78.2%.


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