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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

1999, Number 2

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Rev Mex Patol Clin Med Lab 1999; 46 (2)

Volumetric capillary cytometry. New alternative in determination of lymphocyte subpopulations

Castillo TNP, Barriga AG, Arumir EC, Aceves RA

Language: Spanish
References: 13
Page: 69-73
PDF size: 95.78 Kb.


ABSTRACT

We compared the performance of the IMAGN 2000 System (Biometric Imagin Co) volumetric capillary cytometry method which provides absolute enumeration of CD4+ and CD8+ lymphocytes counts with a flow cytometry method Coulter Epics Profile II (Coulter Co) in 29 AIDS patients specimens that were tested by both methods. This evaluation demonstrated that the Imagn 2000 system provides CD4+ and CD8+ counts in AIDS patients comparable to flow cytometry in terms of accuracy and reproducibility; and the advantages of fully automated operation, easy use, simple instrumentation, lack of a requirement for highly trained personnel or additional hematology equipment and suggests that the volumetric capillary cytometry offers a practical alternative to flow cytometry for the determination of absolute CD4+ and CD8+ T cell counts in HIV infected persons. This technology may be particularly well suited for small laboratories, clinics and hospitals that not currently performing flow cytometry.


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