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2009, Number 4

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Rev Inst Nal Enf Resp Mex 2009; 22 (4)

Effect of an electrolyzed superoxidized solution with neutral pH on the influenza A virus infection in MDCK cells

Cabello GC, Rosete ODP, Manjarrez ZME

Language: Spanish
References: 34
Page: 280-287
PDF size: 97.09 Kb.


ABSTRACT

Background: Influenza viruses produce acute respiratory infections and cause high rates of morbidity and mortality in all age-groups. Their transmission is by expelled saliva droplets which can be deposited in hands and objects. For disinfection of surfaces and working materials, disinfectants such as detergents and chloride and, more recently, electrolyzed superoxidized solution (ESS) are used. The latter is prepared with purified water, saturated sodium chloride solution, and electrolyzed with neutral pH.
Objective: The aim of the present work was to evaluate the effect of ESS on the influenza A virus.
Material and methods: Nine different concentrations of ESS were prepared and then mixed during 10 to 15 min with a constant infectious dose of the virus, which was subsequently inoculated to MDCK cells. The cytopathic capacity of the virus was evaluated, and the minimal inhibitory dose of ESS was calculated. In order to corroborate the effect of ESS, detections of viral antigens by indirect immunofluorescence and of viral genome by RT-PCR were done.
Results: All concentrations of ESS diminished infectivity of the influenza virus. The lowest concentration (0.47 mg) reduced the viral cytopathic capacity by 40.8%, whereas the highest concentration (37.5 mg) reduced it up to 96% and precluded detection of viral antigens and genome.
Conclusion: ESS diminished the influenza virus infectivity on MDCK cells.


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