Montańo RM, Cisneros LJ, Pedraza-Chaverri J, Becerril BC, Mendoza MC, Ramos AC

Language: Spanish
References: 36
Page: 272-279
PDF size: 90.84 Kb.

ABSTRACT

Introduction: Domestic exposure to biomass smoke (mainly wood) represents the second cause of COPD. About 45% of the world’s population use solid biomass fuels, and 69% of Mexican households at rural and marginalized urban areas use wood as primary fuel. Pathogenetic mechanisms in biomass smoke-associated COPD are only partially known. Recently, a COPD model was developed at INER by exposing guinea pigs to wood smoke. This model showed enhanced expression and activity of elastolytic, collagenolytic and gelatinolytic enzymes, pulmonary hypertension, smooth muscle hyperplasia, and apoptosis after. However, the status of oxidative stress or antioxidant enzymes was not reported.
Objective: To analyze plasma concentration of malondialdehyde (MDA) and activity of SOD in bronchoalveolar lavage (BAL) macrophages in guinea pigs daily exposed to the smoke produced by 60 g of wood/day during 7 months wood during 7 months.
Methods: MDA was measured by the 1-methyl-2-phenylindole reaction and SOD activity with a commercial Kit.
Results: MDA content increased after wood smoke exposure. Thus, while control groups had ~0.459 ± 0.051 nmol/ml MDA, experimental groups reached 2.626 ± 0.310, 1.707 ± 0.202, and 1.354 ± 0.109 nmol/ml at 3, 5 and 7 months of exposure, respectively (p ‹ 0.001 at all comparisons). SOD activity was also increased, changing from ~0.016 ± 0.002 units/10⁶ BAL macrophages in control groups to 0.071 ± 0.006, 1.121 ± 0.049, and 0.276 ± 0.033 units/10⁶ BAL macrophages at 3, 5 and 7 months, respectively (p ‹ 0.001 at all comparisons).
Conclusion: Our findings suggest that oxidative stress plays a role in wood smoke-induced COPD in guinea pigs.

REFERENCES

1. Global Initiative for Chronic Obstructive Lung Disease. Global Strategy for the Diagnosis, Management and Prevention of Chronic Obstructive Pulmonary Disease. Access date: -III-2008. Date last updated: December 2007. Available from: www.goldcopd.com/Guideline Resources.asp?l152&l250.

2. Celli BR, MacNee W; ATS/ERS Task Force. Standards for the diagnosis and treatment of patients with COPD: a summary of the ATS/ERS position paper. Eur Respir J 2004;23:932-946.

3. Shapiro SD, Ingenito EP. The pathogenesis of chronic obstructive pulmonary disease: advances in the past 100 years. Am J Respir Cell Mol Biol 2005;32:367-372.

4. Barnes PJ, Shapiro SD, Pauwels RA. Chronic obstructive pulmonary disease: molecular and cellular mechanisms. Eur Respir J 2003;22:672-688.

5. Barnes PJ. Mediators of chronic obstructive pulmonary disease. Pharmacol Rev 2004;56:515-548.

6. Ofulue AF, Ko M, Abboud RT. Time course of neutrophil and macrophage elastinolytic activities in cigarette smoke-induced emphysema. Am J Physiol 1998;275(6 Pt 1):L1134-L1144.

7. Rahman I. Oxidative stress in pathogenesis of chronic obstructive pulmonary disease: cellular and molecular mechanisms. Cell Biochem Biophys 2005;43:167-188.

8. Lopez AD, Shibuya K, Rao C, et ál. Chronic obstructive pulmonary disease: current burden and future projections. Eur Respir J 2006;27:397-412.

9. Rabe KF, Hurd S, Anzueto A, et ál. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary. Am J Respir Crit Care Med 2007;176:532-555.

10. Rennard SI, Vestbo J. COPD: the dangerous underestimate of 15%. Lancet 2006;367:1216-1219.

11. Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol 1997;82:291-295.

12. Leeuwenburgh Ch, Hansen P, Shaish A, Holloszy JO, Heinecke JW. Markers of protein oxidation by hydroxyl radical and reactive nitrogen species in tissues of aging rats. Am J Physiol Regul Integr Comp Physiol 1998;274:R453-R461.

13. Montańo M, Beccerril C, Ruiz V, Ramos C, Sansores RH, Gonzalez-Avila G. Matrix metalloproteinases activity in COPD associated with wood smoke. Chest 2004;125:466-472.

14. Perez-Padilla R, Regalado J, Vedal S, et ál. Exposure to biomass smoke and chronic airway disease in Mexican women: a case control study. Am J Respir Crit Care Med 1996;154(3 Pt 1):701-706.

15. Ramirez-Venegas A, Sansores RH, Perez-Padilla R, et ál. Survival of patients with chronic obstructive pulmonary disease due to biomass smoke and tobacco. Am J Respir Crit Care Med 2006;173:393-397.

16. Sandoval J, Salas J, Martinez-Guerra ML, et ál. Pulmonary arterial hypertension and cor pulmonale associated with chronic domestic woodsmoke inhalation. Chest 1993;103:12-20.

17. Delgado J, Martinez LM, Sanchez TT, Ramirez A, Iturria C, Gonzalez- Avila G. Lung cancer pathogenesis associated with wood smoke exposure. Chest 2005;128:124-131.

18. Desai MA, Mehta S, Smith KR. Indoor smoke from solid fuels: Assessing the environmental burden of disease at national and local levels. Geneva, World Health Organization, 2004. (WHO Environmental Burden of Disease Series, No. 4).

19. Orozco-Levi M, Garcia-Aymerich J, Villar J, Ramirez-Sarmiento A, Anto JM, Gea J. Wood smoke exposure and risk of chronic obstructive pulmonary disease. Eur Respir J 2006;27:542-546.

20. Fujita M, Nakanishi Y. The pathogenesis of COPD: lessons learned from in vivo animal models. Med Sci Monit 2007;13:RA19-RA24.

21. Fehrenbach H. Animal models of pulmonary emphysema: a stereologist’s perspective. Eur Respir Rev 2006;15:136-147.

22. Groneberg DA, Chung KF. Models of chronic obstructive pulmonary disease. Respir Res 2004;5:18.

23. Ramos C, Cisneros J, Gonzalez-Avila G, Becerril C, Ruiz V, Montańo M. Increase of matrix metalloproteinases in woodsmoke-induced lung emphysema in guinea pigs. Inhal Toxicol 2009;21:119-132.

24. Gérard-Monnier D, Erdelmeier I, Régnard K, Moze-Henry N, Yadan JC, Chaudičre J. Reactions of 1-methyl-2-phenylindole with malondialdehyde and 4-hydroxyalkenals. Analytical applications to a colorimetric assay of lipid peroxidation. Chem Res Toxicol 1998;11:1176-1183.

25. Agusti A, Soriano JB. COPD as a systemic disease. COPD 2008;5:133-138.

26. Petty TL. Pulmonary rehabilitation of early COPD. COPD as a systemic disease. Chest 1994;105:1636-1637.

27. Cobo AC, Fabián SMMG. Tratamiento de la hipertensión arterial sistémica en pacientes con asma y enfermedad pulmonar obstructiva crónica. Rev Inst Nal Enf Resp Mex 2007;20:64-70.

28. Iik B, Iik RS, Akyildiz L, Topçu F. Does biomass exposure affect serum MDA levels in women? Inhal Toxicol 2005;17:695-697.

29. Kluchová Z, Petrásová D, Joppa P, Dorková Z, Tkácová R. The association between oxidative stress and obstructive lung impairment in patients with COPD. Physiol Res 2007;56:51-56.

30. Ceylan E, Kocyigit A, Gencer M, Aksoy N, Selek S. Increased DNA damage in patients with chronic obstructive pulmonary disease who had once smoked or been exposed to biomass. Respir Med 2006;100:1270-1276.

31. Barregard L, Sällsten G, Andersson L, et ál. Experimental exposure to wood smoke: effects on airway inflammation and oxidative stress. Occup Environ Med 2008;65:319-324.

32. Agacdiken A, Basyigit I, Ozden M, et ál. The effects of antioxidants on exercise-induced lipid peroxidation in patients with COPD. Respirology 2004;9:38-42.

33. Joppa P, Petrásová D, Stancák B, Dorková Z, Tkácová R. Oxidative stress in patients with COPD and pulmonary hypertension. Wien Klin Wochenschr 2007;119: 428-434.

34. Drost EM, Skwarski KM, Sauleda J, et ál. Oxidative stress and airway inflammation in severe exacerbations of COPD. Thorax 2005;60:293-300.

35. Kluchová Z, Petrásová D, Joppa P, Dorková Z, Tkácová R. The association between oxidative stress and obstructive lung impairment in patients with COPD. Physiol Res 2007;56:51-56.

36. Montańo M, Cisneros J, Ramírez-Venegas A, et ál. Malondialdehyde and superoxide dismutase correlate with FEV1 in patients with COPD associated with wood smoke exposure and tobacco smoking. Inhal Toxicol. En prensa 2010.