Corona-Oregón E, Conde-González CJ, Barrón BL, Sánchez-Alemán MÁ

Language: Spanish
References: 24
Page: 98-102
PDF size: 90.30 Kb.

ABSTRACT

Objective: To study the factors associated with the presence of antibodies against herpes simplex virus type 1 (HSV-1) and to screen for HSV-1 in genital samples.
Materials and methods: Students answered a survey and provided biological samples (blood and genital discharge). The detection of IgG and IgM antibodies against HSV-1 was performed by an ELISA test. From IgM positive samples we sought and extracted genital DNA and identified a beta-globulin gene and HSV-1.
Results: Eight hundred and fifteen students participated. IgG/HSV-1 seroprevalence was 56.7%, HSV-1 infection was associated with number of sexual partners, exchanging sex for money, same sex relationships and occasional partners. IgM/HSV-1 seroprevalence was 18.2%, 91 samples were positive for human beta-globuline but none for HSV-1 DNA.
Conclusions: Epidemiological evidence suggests that HSV-1 could be transmitted by sexual contact among college students; however, HSV-1 was not detected in any of the genital samples analyzed. To further test our hypothesis we need to study HSV-1 among high risk groups or increase our sample size.

REFERENCES

1. Whitley RJ, Roizman B. Herpes simplex virus infections. Lancet 2001;357:1513-1518.

2. Nahmias AJ, Lee FK, Beckman-Nahmias S. Sero-epidemiological patterns of herpes simplex virus infection in the world. Scand J Infect Dis 1990;69:19-36.

3. Roberts CM, Pfister JR, Spear SJ. Increasing proportion of herpes simplex virus type 1 as a cause of genital herpes infection in college students. Sex Transm Dis 2003;30:797-800.

4. Thompson C. Genital herpes simplex typing in genitourinary medicine:1995- 1999. Int J STD AIDS 2000;11:501-502.

5. Nilsen A, Myrmel H. Changing trends in genital herpes simplex virus infection in Bergen, Norway. Acta Obstet Gynecol Scand 2000;79:693-696.

6. Scoular A, Norrie J, Gillespie G, Mir N, Carman WF. Longitudinal study of genital infection by herpes simplex virus type 1 in western Scotland over 15 years. BMJ 2002;324:1366-1367.

7. Tran T, Druce JD, Catton MC, Kelly H, Birch CJ. Changing epidemiology of genital herpes simplex virus infection in Melbourne, Australia, between 1980 and 2003. Sex Transm Infect 2004;80:277-279.

8. Löwhagen G-B, Tunbäck P, Andersson K, Bergström T, Johannisson G. First episodes of genital herpes in a Swedish STD population: a study of epidemiology and transmission by the use of herpes simplex virus (HSV) typing and specific serology. Sex Transm Infect 2000;76:179-182.

9. Lafferty WE, Downey L, Celum C, Wald A. Herpes simplex virus type 1 as a cause of genital herpes: impact on surveillance and prevention. J Infect Dis 2000;181:1454-1457.

10. Hashido M, Lee FK, Nahmias AJ, Tsugami H, Isomura S, Nagata Y, et al. An epidemiological study of herpes simplex virus type 1 and 2 infection in Japan on type-specific serological assays. Epidemiol Infect 1998;120:170-186.

11. Lamey P, Hyland PL. Changing epidemiology of herpes simplex virus type 1 infections. Herpes 1999;6:20-24.

12. Wagner H, Van Dyck E, Roggen, Nunn AJ, Kamali A, Schmid DS, et al. Seroprevalence and Incidence of sexually transmitted diseases in rural Ugandan population. Int J STD AIDS 1994;5:332-337.

13. Cowan FM, French RS, Mayaud P, Gopal R, Robinson NJ, Artimos-de Oliveira S, et al. Seroepidemiological study of herpes simplex virus types 1 and 2 in Brazil, Estonia, India, Morocco, and Sri Lanka. Sex Transm Infect 2003;79:286-290.

14. Herrera-Martínez E, Ondarza-Aguilera R, Estrada-Parra S, Pérez G, Barrón BL. Comparison ot two methods of PCR followed by enzymatic restriction digestion for detection and typing of herpes simplex viruses isolated from patients with mucocutaneous or cutaneous lesions. Rev Latinoam Microbiol 2005;47:76-81.

15. Bauer HM, Greer CE, Manos MM. Determination of genital human papillomavirus infection using consensus PCR, p. 132-152. En: Herrington CS, McGee JOD, editors. Diagnostic molecular pathology: a practical approach. Oxford, UK: Oxford University Press; 1992.

16. Madhavan HN, Priya K, Bagyalakshmi R. Phenotypic and genotypic methods for the detection of herpes simplex virus serotypes. J Virol Methods 2003;108:97-102.

17. Pebody RG, Andrews N, Brown D, Gopal R, de Melker H, Francois G, et al. The seroepidemiology of herpes simplex virus type 1 and 2 in Europe. Sex Tranms Infect 2004;80:185-191.

18. Hashido M, Kawana T, Matsunaga Y, Inouye S. Changes in prevalence of herpes simplex virus type 1 and 2 antibodies from 1973 to 1993 in the rural district of Japan. Microbiol Immunol 1999;43:177-180.

19. Stock Ch, Guillén-Grima F, Hermoso-de Mendoza J, Marín-Fernández B, Aguinaga-Ontoso I, Krämer A. Risk factors of herpes simplex type 1 (HSV-1) infection and lifestyle factors associated with HSV-1 manifestations. Eur J Epidemiol 2001;17:885-890.

20. Gibson JJ, Hornung CA, Alexander GR, Lee FK, Potts WA, Nahmias AJ. A Cross-sectional study of herpes simplex virus types 1 and 2 in college students: ocurrence and determinants of infection. J Infect Dis 1990;162:306-312.

21. Gutiérrez G, Ruiz-Gómez J. Seroepidemiology of 10 infectious diseases in children in Mexico City. Measles, rubella, parotitis, typhoid, whooping cough, amebiasis, influenza and adenovirus infections, Mycoplasma pneumoniae and herpes simplex. Gac Med Mex 1973;105:529-540.

22. Roizman B, Knipe DM, Whitley RJ. The replication of Herpes simplex viruses. En: Knipe DM, Howley P, Griffin DE, editors. Fields’ virology. Fifth edition. New York: Lippincott-Williams and Wilkins; 2007. pp. 2501-2601.

23. Conde-Glez CJ, Juárez-Figueroa L, Uribe-Salas F, Hernández-Nevárez P, Schmid DS, Calderón E, Hernández-Ávila M. Analysis of Herpes simplex virus 1 and 2 infection in women with high risk sexual behaviour in Mexico. Int J Epidemiol 1999;28:571-576.

24. Smith JS, Robinson NJ. Age-Specific Prevalence of Infection with Herpes Simplex Virus Types 2 and 1: A Global Review. J Infect Dis 2002;186(Suppl 1):S3-28.