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2009, Number 3

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Rev Inst Nal Enf Resp Mex 2009; 22 (3)

Distribution of myeloid and plasmacytoid dendritic cells from bronchoalveolar lavage of patients with hypersensitivity pneumonitis

Barrera L, Sada-Ovalle I, Vázquez CL, Bañales MJL, Barrera A, Torre-Bouscoulet L, Mendoza F

Language: Spanish
References: 26
Page: 154-162
PDF size: 216.85 Kb.


ABSTRACT

Background: Hypersensitivity pneumonitis (HP) is a diffuse inflammatory disease of lung parenchyma resulting from repetitive inhalation of organic particles. Dendritic cells and their precursors play an important role not only as antigen presenting cells but also as part of an immunoregulatory network. Depending on their lineage and stage of differentiation and activation, dendritic cells can promote a strong T-lymphocytes-mediated immunological response or an anergy state.
Objective: To phenotypically characterize myeloid (mDCs) and plasmacitoid (pDCs) dendritic cells recovered in bronchoalveolar lavage from patients with subacute or chronic HP, and to compare the results with that obtained in patients with idiopathic pulmonary fibrosis (IPF) and healthy controls.
Methods: BAL cells from 8 patients with subacute HP, 8 with chronic HP, 8 with IPF and 4 healthy subjects were used. The phenotype of dendritic cells subpopulations were characterized by means of flow cytometry.
Results: In all studied groups a predominance of mDCs (HLA-DR+D11c+), as compared with pDCs, was observed. A lower frequency of both mDCs and pDCs was documented in subacute HP patients vs. healthy controls (5.1 ± 1.1% [mean ± SD] vs. 30.4 ± 5.2%, p ‹ 0.01, and 0.5 ± 0.2% vs. 5.8 ± 2.4%, p ‹ 0.05, respectively). Compared with healthy subjects, in the cell populations from HP and IPF patients there was no expression of activation markers CD40 and CD80.
Conclusions: The low frequency of mDCs and pDCs, along with the absence of activation or maturation markers, suggests that patients with HP, mainly in the subacute form, present a T lymphocyte anergy state.


REFERENCES

  1. Selman M, Chapela R, Raghu G. Hypersensitivity pneumonitis: clinical manifestations, diagnosis, phatogenesis and therapeutic strategies. Semin Resp Med 1993;14:353-364.

  2. Selman M, Terán L, Mendoza A, et ál. Increase of HLA-DR7 in pigeon breeder´s lung in a Mexican population. Clin Immunol Immunopathol 1987;44:63-70.

  3. Camarena A, Juárez A, Mejía M, et ál. Major histocompatibility complex and tumor necrosis factor-alpha polymorphisms in pigeon breeder´s disease. Am J Respir Crit Care Med 2001;163:1528-1533.

  4. Selman M. Hypersensitivity pneumonitis. In: Schwars M, King T, editors. Interstitial lung diseases. Hamilton Ontario: BC Decker; 1998.p.393-422.

  5. Pepys J. Clinical and therapeutic significance of patterns of allergic reactions of the lungs to extrinsic agents: the 1977 J. Burns Amberson Lecture. Am Rev Respir Dis 1977;116:573-588.

  6. Barrera L, Mendoza F, Zúñiga J, et ál. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis. Am J Respir Crit Care Med 2008;177:44-55.

  7. Lambrecht BN, De Veerman M, Coyle AJ, Gutierrez-Ramos JC, Thielemans K, Pauwels RA. Myeloid dendritic cell induce Th2 responses to inhaled antigen, leading to eosinophilic airway inflammation. J Clin Invest 2000;106:551-559.

  8. Kalin´ ski P, Hilkens CM, Wierenga EA, Kapsenberg ML. T- cell priming by type-1 and type-2 polarized dendritic cells: the concept of a third signal. Immunol Today 1999;20:561-567.

  9. Macatonia SE, Hosken NA, Litton M, et ál. Dendritic cells produce IL-12 and direct the development of Th1 cells from naive CD4+ T cells. J Immunol 1995;154:5071-5079.

  10. Tanaka H, Demeure CE, Rubio M, Delespesse G, Sarfati M. Human monocyte-derived dendritic cells induce naive T cells differentiation into T helper cell type 2 (Th2) or Th1/Th2 effectors. Role of stimulator/responder ratio. J Exp Med 2000;192:405-412.

  11. Bellini A, Vittori E, Marini M, Ackerman V, Mattoli S. Intraephitelial dendritic cells and selective activation of Th2-like lymphocytes in patients with atopic asthma. Chest 1993;103:997-1005.

  12. Bertorelli G, Bocchino V, Zhou X, et ál. Dendritic cell number is related to IL-4 expression in the airways of atopic asthmatic subjects. Allergy 2000;55:449-454.

  13. Upham JW, Word L, Demburg JA. Circulating dendritic cells and asthma: rapid response to inhaled allergen. Am J Respir Crit Care Med 1999;159:854(a).

  14. Nicod LP, Lipscomb MF, Weissler JC, Lyons CR, Albertson J, Toews GB. Mononuclear cells in human lung parenchyma. Characterization of a potent accessory cell not obtained by bronchoalveolar lavage. Am Rev Respir Dis 1987;136:818-823.

  15. Donnenberg VS, Donnenberg AD. Identification, rare- event detection and analysis of dendritic cell subsets in bronchoalveolar lavage fluid and peripheral blood by flow cytometry. Front Biosci 2003;8:s1175-s1180.

  16. Bustos ML, Frías S, Ramos S, et ál. Local and circulating microchimerism is associated with hypersensitivity pneumonitis. Am J Respir Crit Care Med 2007;176:90-95.

  17. Selman M, Pardo A, Barrera L, et ál. Gene expression profiles distinguish idiopathic pulmonary fibrosis from hypersensitivity pneumonitis. Am J Respir Crit Care Med 2006;173:188-198.

  18. Sandoval J, Bañales JL, Cortés JJ, Mendoza F, Selman M, Reyes PA. Detection of antibodies against avian antigens in bronchoalveolar lavage from patients with pigeon breeder’s disease: usefulness of enzyme-linked immunosorbent assay and enzyme immunotransfer blotting. J Clin Lab Anal 1990;4:81-85.

  19. Mathew S, Bauer KL, Fischoeder A, Bhardwaj N, Oliver SJ. The anergic state in sarcoidosis is associated with diminished dendritic cell function. J Immunol 2008; 181:746-755.

  20. Lommatzsch M, Bratke K, Bier A, et ál. Airway dendritic cell phenotypes in inflammatory diseases of the human lung. Eur Respir J 2007;30:878-886.

  21. Demedts IK, Bracke KR, Van Pottelberge G, et ál. Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med 2007;175:998-1005.

  22. Bratke K, Lommatzsch M, Julius P, et ál. Dendritic cell subsets in human bronchoalveolar lavage fluid after segmental allergen challenge. Thorax 2007;62:168-175.

  23. Cella M, Jarrossay D, Facchetti F, et ál. Plasmacytoid monocytes migrate to inflammed lymph nodes and produce large amounts of type I interferon. Nat Med 1999;5:919-923.

  24. Turiaf J, Menault M, Battesti JP. Research on immunologic manifestations of sarcoidosis. Ann Med Interne (Paris) 1970;121:117-134.

  25. Mariotti S, Teloni R, Iona E, et ál. Mycobacterium tuberculosis subverts the differentiation of human monocytes into dendritic cells. Eur J Immunol 2002;32: 3050-3058.

  26. Granelli-Piperno A, Golebiowska A, Trumpfheller C, Siegal FP, Steinman RM. HIV-1 infected monocyte-derived dendritic cells do not undergo maturation but can elicit IL-10 production and T cell regulation. Proc Natl Acad Sci U S A 2004;101:7669-7674.




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