medigraphic.com

2009, Number 4

<< Back

Vet Mex 2009; 40 (4)

Analysis of the parameters of the ejaculate in the laboratory Wistar rat: technical description

Lucio RA, Tlachi JL, López AA, Zempoalteca R, Velázquez-Moctezuma J

Language: English/Spanish
References: 24
Page: 405-415
PDF size: 223.15 Kb.


ABSTRACT

The number of spermatozoa, weight and size of the seminal plug has been quantified in the ejaculate of the laboratory rat and sperm viability, sperm mobility and sperm concentration in samples obtained from the epididymis. The reference values for the rat ejaculate have not been determined maybe due to the difficulty to obtain it directly from the male. Nevertheless, an approach can be obtained analyzing the ejaculate collected from the inseminated female. The objective of this study was to evaluate and propose the values of the macroscospic and microscopic parameters of the semen and seminal plug obtained from the female. The seminal analysis of the Wistar rats was performed adapting the methods used for other species. After one ejaculatory series, semen contained in the uterine horns was obtained, as well as the seminal plug from the vagina. In more than one hundred ejaculates, 94.54% were off-white with 2.35 ± 0.06 mm of viscosity and 8.13 ± 0.02 of pH. The sperm concentration was 16.3 ± 0.59 millions of spermatozoa per mL, 0.72 ± 0.01 mobility index, 64.4 ± 0.7% viability and 99.11 ± 0.20% normal morphology. From 99% of seminal plugs, 92.7% were hardened, they weighed 115.63 ± 1.54 mg, measured 12.41 ± 0.13 and 5.31 ± 0.05 mm of length and width respectively, and volume 87.70 ± 1.74 mm3. In conclusion, the used method to obtain and to evaluate the parameters of the ejaculate is reliable, for that reason, it is suggested that the results obtained could be considered as indicative values for semen and seminal plug for the Wistar rat


REFERENCES

  1. 1.FARRIS EJ. Spermatozoa counts in albino rats. Anat Rec 1946; 94:360-361.

  2. 2.BLANDAU RJ, ODOR DL. The total number of spermatozoa reaching various segments of the reproductive tract in the female albino rat at intervals after insemination. Anat Rec 1949; 103:93-109.

  3. 3.POUND N, GAGE MJG. Prudent sperm allocation in Norway rats, Rattus norvegicus: a mammalian model of adaptive ejaculate adjustment. Anim Behav 2004; 68:819-823.

  4. 4.AUSTIN D, DEWSBURY DA. Reproductive capacity of male laboratory rats. Physiol Behav 1985; 37:627-632.

  5. 5.MOTRICH RD, PONCE AA, RIVERO VE. Effect of tamoxifen treatment on the semen quality and fertility of the male rat. Fertil Steril 2007; 2:452-461.

  6. 6.SCOTT JW, DZIUK PJ. Evaluation of the electroejaculation technique and the spermatozoa thus obtained from rats, mice and guinea pigs. Anat Rec 1959: 133:655-664.

  7. 7.AGMO A. The number of spermatozoa in spontaneous ejaculates in rats. J Reprod Fertil 1976; 48:405-407.

  8. 8.AGMO A, ANDERSSON R, JOHANSSON C. Effect of oxytocin on sperm numbers in spontaneous rat ejaculation. Biol Reprod 1978; 18:346-349.

  9. 9.LAMANO-CARVALHO TL, KEMPINAS WG. Determination of spermatozoa concentration in the rat cauda epididymis. Braz J Med Biol Res 1987; 20:429-431.

  10. 10.ROBB GW, AMANN RP, KILLIAN GJ. Daily sperm production and epididymal sperm reserves of pubertal and adult rats. J Reprod Fertil 1978; 54:103-107.

  11. 11.GOYAL HO, BRADEN TD, MANSOUR M, WILLIAMS CS, KAMALELDIN A, SRIVASTAVA KK. Diethylbestrol- treated adult rats with altered epididymal sperm numbers and sperm mobility parameters but without alterations in sperm production and sperm morphology. Biol Reprod 2001; 64:927-934.

  12. 12.SYNTIN P, ROBAIRE B. Sperm structural and mobility changes during aging in the brown Norway rat. J Androl 2001; 22:235-244.

  13. 13.DOSTAL LA, FABER CK, ZANDEE J. Sperm motion parameters in vas deferens and cauda epididymal rat sperm. Reprod Toxicol 1996; 3:231-235.

  14. 14.LUCIO RA, MANZO J, MARTINEZ-GOMEZ M, SACHS BD, PACHECO P. Participation of pelvic nerve branches in male rat copulatory behavior. Physiol Behav 994; 55:241-246.

  15. 15.MANZO J, VAZQUEZ MI, CRUZ MR, HERNANDEZ ME, CARRILLO P, PACHECO P. Fertility ratio in male rats: effects after denervation of two pelvic floor muscles. Physiol Behav 2000; 5:611-618.

  16. 16.TLACHI-LÓPEZ JL, ZEMPOALTECA R, LUCIO RA. Determinación de los parámetros macroscópicos y microscópicos del semen y las características del tapón seminal de rata. Memorias de Décimo Curso Internacional: Bases Biológicas de la Conducta. Resumen; junio, 10; Tlaxcala (Tlaxcala) México. Universidad Autónoma de Tlaxcala, 2005:1-23.

  17. 17.WORLD HEALTH ORGANIZATION. Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge: Cambridge University Press, 2000.

  18. 18.BLANDAU RJ. On the factors involved in sperm transport through the cervix uteri of the albino rat. Am J Anat 1945; 77:253-272.

  19. 19.MCCLINTOCK MK, ANISKO JJ, ADLER NT. Group mating among Norway rats II. The social dynamics of copulation: competition, cooperation and mate choice. Anim Behav 1982; 30:410-425.

  20. 20.CANCEL AM, LOBDELL D, MENDOLA P, PERREAULT SD. Objective evaluation of hyperactivated motility in rat spermatozoa using computer-assited sperm analyzis. Human Reprod 2000; 15:1322-1328.

  21. 21.HOWARTH B JR. Oxidation of [U-14C] palmitic acid by cock spermatozoa. Poult Sci 1978; 57:1751-1754.

  22. 22.ALLEN WE. Fertilidad y Obstetricia Equina. México DF: Ed. ACRIBIA, 2001.

  23. 23.SACHS BJ Role of striated penile muscles in penile reflexes, copulation and induction of pregnancy in the rat. J Reprod Fertil 1982; 66:433-443.

  24. 24.QUINLAN DM, NELSON R, PARTIN AW, MOSTWIN JL, WALSH PC. The rat as a model for the study ofpenile erection. J Urol 1989; 141:656-661.




2020     |     www.medigraphic.com