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1994, Number 3

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Vet Mex 1994; 25 (3)

Effect of the addition of Saccharomyces cerevisiae and urea-molasses on in vivo and in situ digestibility in diets for sheep based on sesame straw

Ayala OJ, Mendoza MGD, Bárcena GR, González MSS

Language: English/Spanish
References: 30
Page: 221-226
PDF size: 553.10 Kb.


ABSTRACT

Two experiments were conducted in order to evaluate changes on sesame straw (SS) digestibility using a molasses (9%) - urea (1%) supplement (Mu) and a probiotic, Saccharomyces cerevisiae (PRO). In experiment 1, fiber (NDF) in situ digestibility (ISD) was determined using 12 ruminally cannulated sheep (45 + 508 kg), Where samples (5g SS) in nylon bags, were incubated at 6, 24, 48, 72 and 96 h, in four periods. Treatments were assigned in a factorial arrangement (2 x 2 x 3), where factors consisted on two levels of P (0 or .5 g/animal/day), two levels of MU (0 or 10%), combined with three levels of SS (50, 60 and 70%; plus the concentrate). Fiber ISD was increased (P ‹ .05) by the PRO and the MU at all incubation times; also by 50% SS compared to 60 and 70% SS. In Experiment 2, in vivo digestibility (IVD) was studied using 70% SS (plus 30 or 20% concentrate), two levels of PRO (0 or .5 g/animal/day) and MU (0 or 10%), and one control (T5) ration (40% SS, 30% alfalfa hay and 30% concentrate), in a complete randomized design with 3 sheep by treatment). Treatments were: T1, MU 0% + PRO 0%; T2, MU 0% + PRO .5%; T3, MU 10% + PRO 0% and T4, 10% + PRO .5. Fiber IVD (%) was different (P ‹ .05): 66.8a (T4), 61.9ab (T5), 52.2bc (T3), 52.1bc (T2) and 41.5c (T1). Intake and ruminal pH did not change (P › .05). Protozoa/ml x 103 was different (P ‹ .05): 1128a (T4), 950ab (T5), 900ab (T3), 764b (T2) and 771b (T1). Therefore, both the probiotic and the supplement increased fiber digestibility and number of protozoa which could be associated with the increment in fiber digestibility.


REFERENCES

  1. Association of Official Analytical Chemists: Official Methods of Analysis of the Association of Official Analytical Chemists. 13th ed. Association of Official Analytical Chemists, Washington, D.C., 1980.

  2. Bartley, E. and Deyoe, C.: Reducing the rate of ammonia released by the use of alternative non-protein nitrogen sources. In: Recent Developments in Ruminant Nutrition. Edited by: Haresign, W., Cole, D.J.A., 99-114. Butterworths, London, 1981.

  3. Bonhomme, A.: Rumen ciliates: Their metabolism and relationship with bacteria and their hosts. Anim. Feed Sci. Technol., 30: 203-266 (1990).

  4. Britton, R.A. and Stock, R.A.: Acidosis, rate of starch digestion and intake. Proceedings of the Feed Intake by Beef Cattle Symposium. MP 121. Stillwater, Oklahoma. 1986. 125-136. Agricultural Experiment Station. Oklahoma State University. Stillwater, Oklahoma (1987).

  5. Dawson, K.A.: Mode of action of yeast culture,in the rumen: A fermentation modifier. Biotechnology in the Feed Industry: Proceedings of Alltech's Third Annual Symposium. Nicholasville, Kentucky. 1987. 35-39. Alltech Technical Publications. Nicholasville, Kentucky (1987).

  6. Dehority, B.A.: Evaluating of subsampling and fixation procedures used for counting rumen ciliate protozoa. J. Gen. Microbiol., 48: 182-185 (1984).

  7. Djajanegara, A. and Doyle, P.T.: Urea supplementation compared with pretreatment. 1. Effects on intake, digestion and liveweight change by sheep fed a ricestraw. Anim. Feed Sci. Technol., 27: 17-30 (1989).

  8. Edwards, I.: Practical uses of yeast culture in beef production: Insight into its mode of action. Biotechnology in the Feed Industry: Alltech's Seventh Annual Symposium. Nicholasville, Kentucky. 1991. 51-64. Alltech Technical Publications. Nicholasville, Kentucky (1991).

  9. Erasmus, L.J.: The importance of duodenal amino acid profiles for dairy cows and the significance of changes in these profiles following the use of Yea-Sacc1026. Biotechnology in the Feed Industry: Alltech's Seventh Annual Symposium. Nicholasville, Kentucky. 1991. 35-50 Alltech Technical Publications. Nicholasville, Kentucky (1991).

  10. Frumholtz, P.P., Newbold, C.J. and Wallace, R.J.: Influence of Aspergillus oryzae fermentation extract on the fermentation of a basal ration in the rumen simulation technique Rusitec. J. Agric. Sci. (Camb.), 113: 169-172 (1989).

  11. Gray, W. and Ryan, J.: A study of the effect of yeast culture on ruminal fermentation in sheep. Biotechnology in the Feed Industry: Alltech's Fourth Annual Symposium. Nicholasville, Kentucky. 1988. 129-150. Alltech Technical Publications. Nicholasville, Kentucky (1988).

  12. Harris, L.E.: Métodos para el Análisis Químico y la Evaluación Biológica de Alimentos para Animales. Universidad de Florida, Gainesville, Florida, 1970.

  13. Klopfenstein, T.J. y Stock, R.: Alimentación de bovinos para carne en crecimiento-finalización. Memoria del Curso Intensivo Internacional de Manejo Nutricional de Bovinos en Corrales de Engorda. Montecillo, Edo. de México. 1991. 1-39. Centro de Ganadería, Colegio de Postgraduados. Montecillo, Edo. de México (1991).

  14. Leng, R.A.: Feeding strategies: Early drought. In: Drought Feeding Strategies Theory and Practice. Edited by: Penambul, 21-55. Peel Valley Printery, Armidale, Australia, 1986.

  15. Mackie, R.I., Gilchrist, F.M.C., Roberts, A.M., Hannah, P.E. and Schwartz, H.M.: Microbiological and chemical changes in the rumen during the stepwise adaptation of sheep to high concentrate diets. J. Agric. Sci. (Camb.), 90: 241-250 (1978).

  16. Mould, F.L., Orskov, E.R. and Mann, S.O.: Associative effects of mixed feeds. I. Effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of vario us rough ages. Anim. Feed Sci. Technol., 10: 15-30 (1983/84).

  17. Newbold, J.: Rumen manipulation with ionophores and probiotics. Proceedings of the California Animal Nutrition Conference. Fresno, California. 1990. 23-36. Biozyme Enfer. Fresno, California (1990).

  18. Plata, P.F.: Efecto de un probiótico (Saccharomyces cerevisiae) en la fermentación ruminal, digestibilidad in situ y el consumo en bovinos alimentados con tres niveles de paja de avena. Tesis de maestría en ciencias. Centro de Ganadería. Colegio de Postgraduados. Montecillo, Edo. de México, 1992.

  19. Porte, E., Faichney, G. and Wythes, R.: Grass hays complemented with different nitrogen sources and sugar cane molasses in fattening rations for Hereford young bulls. Aust. J. Agric. Res., 19: 803-811 (1988).

  20. Preston, R.T. y Leng, R.A.: Guías Para los Sistemas Alimenticios. Condrit, Cali, Colombia, 1989.

  21. Riquelme, V.E.: Efectos asociativos en dietas basadas en subproductos agrícolas. Rev. Mex. Prod. Anim., 16: 13-24 (1984).

  22. Roa, V. Ma. L.: Efecto de Saccharomyces cerevisiae y tres tipos de fibra en la degradabilidad ruminal de nutrientes en bovinos. Tesis de maestría en ciencias. Centro de Ganadería. Colegio de Posgraduados. Montecillo, Edo. de México, 1992.

  23. Shultz, T.A., Shultz, E.B. y Chicco, FA.: Racionamiento con nitrógeno no proteínico para corderos en el trópico seco-húmedo. IV Conferencia Mundial de Producción Animal. Buenos Aires, Argentina. 1978. 263-271. AAPA-Hemisferio Sur. Buenos Aires, Argentina (1978).

  24. Steel, R.G. y Torrie, J.H.: Bioestadística. Principios y Procedimientos. 2a ed. McGraw-Hill, México, D.F., 1986.

  25. Soest van, P.J.: Use of detergents in analysis of fibrous feeds. II. A rapid method for determination of fiber and lignin. J. Ass. Agric. Chem., 46: 829-835 (1963).

  26. Varga, G.A. and Whitsel, T.J.: Effect of non-structural to structural carbohydrate ration on rate and extent of nutrient utilization in situ. Anim. Feed Sci. Technol., 32: 275-286 (1991).

  27. Wiedmeier, R., Arambel, M. and Walters, J.: Effect of yeast culture and Aspergillus oryzae fermentation extract on ruminal characteristics and nutrient digestibility. J. Dairy. Sci., 70: 2063-2068 (1987).

  28. Williams, P.E.: The mode of action of yeast culture in ruminants diets: A review of the effect on rumen fermentation patterns. Biotechnology in the Feed Industry. Proceedings of Alltech's 5th Annual Symposium. Nicholasville, Kentucky. 1989. 65-84. Alltech Technical Publications. Nicholasville, Kentucky (1989).

  29. Williams, P.E. and Newbold, C.J.: Rumen probiosis. The effect of novel microorganisms on rumen fermentation and ruminant productivity. In: Recent Advances in Animal Nutrition. Edited by: Haresign, W., Cole, D.J.A., 211-227. Bulterworths, London, 1990.

  30. Williams, P.E., Tait, C.A., Innes, G.M. and Newbold, C.J.: Effects of the inclusion of yeast culture (Saccharomyces cerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. J. Anim. Sci., 69: 3016-3026 (1991).




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