2009, Number 4
<< Back Next >>
Rev Mex Patol Clin Med Lab 2009; 56 (4)
Comparative study of two HIV-1 and HCV viral load tests based on Real Time RT-PCR. Technology
Barriga AG, Solís TM , Aceves RA
Language: Spanish
References: 12
Page: 247-256
PDF size: 92.60 Kb.
ABSTRACT
It was performed a comparative study between two tests in order to determine the viral load to the human immunodeficiency virus 1 and to the hepatitis C virus (HCV) (COBAS AmpliPrep/COBAS. AMPLICOR: HIV-1 Monitor Test and Abbott Real-Time HIV-1 and AmpliPrep/COBAS AMPLICOR HCV Monitor Test V2.0, and Abbott Real-Time HCV Test) in 78 patients suffering from HIV-1 and 61 patients with viral hepatitis type C (HCV) subjected to antiviral therapy. It was obtained a correlation of R = 0.96 for HIV and of R = 0.87 for HCV between the two tests. These coefficients and the fact that the difference between the viral loads from a single sample never went over the 0.5 of the logarithm assure that the technological changes will not impact on the results of the evaluation of the antiviral therapies.
REFERENCES
Manzin A, Solforosi L, Giostra F, Bianchi FB, Bruno S et al. Quantitative analysis of hepatitis C virus activity in vivo in diferent groups of untreated patients. Arch Virol 1997; 142: 465-472.
Gerken G, Rothaar T, Rumi M.G, Soffredini R, Trippler M et al. Performance of the COBAS AMPLICOR HCV MONITOR Test, Version 2.0, an Automated Reverse Transcription-PCR Quantitative System for Hepatitis C Virus Load Determination. J Clin Microbiol 2000; 38 (6): 2210-2214.
Chevaliez S, Bouvier-Alias M, Pawlotsky JM. Performance of the Abbott Real-Time PCR Assay Using m2000sp and m2000rt for Hepatitis C Virus RNA Quantification. J Clin Microbiol 2009; 47 (6): 1726-1732.
Boyer JL, Judge TA, Chang EB, Muggia FM, Shapiro CL et al. NIH Consensus Statement on Management of Hepatitis C: 2002, NIH Consensus and State-of-the-Science Statements2002. Vol 19 (3) June 10-12 2002. Bethesda MD, National Institutes of Health, 2002.
Gallegos-Orozco JF, Loaeza del Castillo A, Fuentes AP. García-Sandoval M, Kershenobich D. Early hepatitis C virus changes and sustained response in patients with chronic hepatitis C treated with peginterferon a-2b and ribavirin. Liver International 2005; 25: 91–95.
Slavenburg S, Lamers MH, Roomer R, de Knegt RJ, van Oijen MGH, Drenth JPH. Current clinical care compared with new Dutch guidelines for hepatitis C treatment. Neth J Med 2009; 67 (5): 177-181.
Nolte SF, Fried WM, Shiffman ML, Ferreira-Gonzalez A, Garrett CT et al. Prospective Multicenter Clinical Evaluation of AMPLICOR and COBAS AMPLICOR Hepatitis C Virus Tests. J Clin Microbiol 2001; 39 (11): 4005-4012.
SSA, CENSIDA. Guía de manejo antirretroviral de las personas con VIH. 4a ed. México: CONASIDA; 2008.
Best SJ, Dax EM. Assays for HIV with improved sensitivity and specificity. Exp Opin Invest Drugs 1997; 6(8): 965-983.
Garcia-Diaz A, Clewley GS, Booth CL, Labett W, Mcallister N, Geretti AM. Comparative Evaluation of the performance of the Abbott Realtime Human Immunodeficiency Virus Type 1 (HIV-1) Assay for Measurement of HIV-1 plasma Viral Load following Automated Specimen Preparation. J Clin Microbiol 2006; 44 (5): 1788-1791.
Sloma CR, Germer JJ, Gerais TM, Mandrekar JN, Shawn MP, Yao JDC. Comparison of the abbott Realtime Human Immunodeficiency Virus Type 1(HIV-1) Assay to the Cobas AmpliPrep/Cobas Taqman HIV-1 test: Workfow, Reliability, and Direct Cost. J Clin Microbiol 2009; 47 (4): 889-895.
Damond F, Roquebert B, Bénard A, Collin G, Miceli M, Yéni P, Brun-Vezinet F. Human Immunodeficiency Virus Type 1(HIV-1) plasma Load Discrepancies between the Roche COBAS AMPLICOR HIV-1 MONITOR Version 1.5. and the Roche COBAS AmpliPrep/COBAS Taqman HIV-1 Assays. J Clin Microbiol 2007; 45 (10): 3436-3438.
Full text