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ISSN 1405-1699 (Print)
Órgano Oficial del Colegio Mexicano de Alergia, Asma e Inmunología Pediátrica y de la Asociación Latinoamericana de Pediatría

2009, Number 2

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Alerg Asma Inmunol Pediatr 2009; 18 (2)

The modulation of the synthesis of IL-2 an IFN-γ by means of human mononuclear cells is associated at the physiologic immunosuppression of the epididymal fluid

Rodríguez-Orozco AR, Mallea SLE, Machado CAJ, Cabrera OV, Carbonell VMA

Language: Spanish
References: 24
Page: 39-46
PDF size: 102.77 Kb.


ABSTRACT

Background and objective: IL-2 and IFN-γ play an important role in lymphocyte proliferation and activation. This study was undertaken to analyze if the inhibitory effect of the epididymal fluid on the immune response is associated with the modulation of the expression of IL-2 and IFN-γ. Material and methods: Mononuclear cells (Ficoll-paque d = 1.077) were isolated from blood from healthy donors and they were stimulated with epididymal fluid from everyone: an organ donor, and a man who was castrated by surgery. Once demonstrated the inhibition of proliferation of mononuclear cells, the levels of IL-2 and IFN-γ was determined by ELISA in supernatants of cultures of mononuclear cells stimulated with epididymal fluid and the results were expressed as inhibition or stimulation index. Results: The inhibitory effect of the epididymal fluid on the mononuclear cells proliferation was not associated with the source of the epididymus and it was associated with potent stimulation of the synthesis of IFN-γ up to 51.6 x 103% in cells treated with 1/4 dilution of the epididymal fluid and with IL-2 synthesis inhibition up to 100% in cells treated with 1/4 dilution of the epididymal fluid. Conclusions: The modulation of the synthesis of IL-2 and IFN-γ is one of the mechanisms involved in the inhibition of the local immune response by epydidimal substances.


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