Montes PRC, Murcia MC, Zarco QL

Language: English/Spanish
References: 52
Page: 117-125
PDF size: 1073.15 Kb.

ABSTRACT

Leghorn hens and New Zealand rabbits were immunized against progesterone, in order to produce antibodies to be used in radioinmunoassay (RIA). Acetone and 2-propanol were used to extract antibodies from the yolk of eggs of immunized hens. Antibody extraction from rabbit blood serum was done by precipitation with amonium sulfate. Antibodies from egg yolk had high affinity and specificity for progesterone. Sensitivity of the RIA established with these antibodies, was 0.27 ng/ml, with intra- and interassay coefficients of variation of 19.6 and 22.2%, respectively. There was a significant correlation (0.94) between the progesterone concentrations determined with egg-yolk antibodies and those found using a commercial kit. Antibodies from rabbit serum had also high affinity and specificity for progesterone. RIA, established with these antibodies, had a sensitivity of 0.23 ng/ml, with intra- and interassay coefflcients of variation of 14.7 and 22.7%, respectively. The correlation between progesterone values determined with rabbit antiserum and those obtained with a commercial kit was 0.96. The yield of antibodies from egg yolk was 2.5 times higher than that of rabbit serum. It is concluded, that egg yolk of immunized hens is a practical source of antiprogesterone antibodies with similar quality to that of antibodies extracted from the blood serum of immunized rabbits.

REFERENCES

1. Abraham, G.E.: Radioimmunoassay of steroids in biological materials. Acta endocrinol. 183 (Suppl.): 1-42 (1974).

2. Abraham, G.E.: Reliability criteria for steroid radioimmunoassay. In: Radioimmunoassay of Steroid Hormones. 2nd ed. Edited by: Gupta, D., 9-17. Verlag Chemie, Weinheim, Germany, 1980.

3. Abraham, G.E., Manlinos, F.S. and Garza, R.: Radioimmunoassay of steroids. In: Handbook of Radioimmunoassay. Edited by: Abraham, G.E., 591-656. Marcel Dekker, New York, 1977.

4. Arnstadt, K.I. and Cleere, W.F.: Enzyme-immunoassay for determination of progesterone in milk from cows. J. Reprod. Fert., 62: 173-180 (1981).

5. Bade, H. and Stegemann, H.: Rapid method of extraction of antibodies from hen egg yolk. J. Immunol. Methods, 72: 421-426 (1984).

6. Benedict, A.A. and Yamaga, K.: Immunoglobulins and antibody production in avian species. In: Comparative Immunology. Edited by: Marchalonis, J.J., 335-375. Blackwell Scientific Publications, Oxford, U.K., 1976.

7. Bosu, W.T.K, Edqvist, LE., Lindberg, P., Martinsson, K. and Johansson, E.D.B.: The effect of various dosages of lynestrenol on the plasma levels of oestrogens and progesterone during the menstrual cycle in the Rhesus monkey. Contraception, 13: 677-684 (1976).

8. Breuel, K.F., Spitzer, J.C., Gimenez, T., Henricks, D.M. and Gray, S.L.: Effect of holding time and temperature of bovine whole blood on concentrations of progesterone, estradiol-17b and estrone in plasma and serum samples. Theriogenology, 30: 613-627 (1988).

9. Capparelli, R., Iannelli, D. and Bordi, A.: Use of monoclonal antibodies for radioimmunoassay of Water Buffalo milk progesterone. J. Dairy Res., 54: 471-477 (1987).

10. Chard, T.: An introduction to radioimmunoassay and related techniques. In: Laboratory Techniques in Biochemistry and Molecular Biology. Vol. 6, Part II. 4th ed. Edited by: Burdon, R.H., Knippenberg van, P.H., 1-247. Elsevier, Amsterdam, 1990.

11. Cooper, T.G.: The Tools of Biochemistry. John Wiley and Sons, New York, 1977.

12. Dawson, E.C., Denissen, E.H.C. and Weemen van, B.K.: A simple and efficient method for raising steroid antibodies in rabbits. Steroids, 31: 357-366 (1978).

13. Diez, T., Manzo, M., Troconiz, J., Benacchio, N. and Verde, O.: Plasma progesterone levels during the estrous cycle of Holstein and Brahman cows. Carora type and cross-bred heifers. Theriogenology, 26: 419-431 (1986).

14. Dobson, H.: A Radioimmunoassay Laboratory Handbook: With Special Reference to Hormones of Reproduction. Liverpool University Press, Liverpool, U.K., 1983.

15. Feldman, H. and Rodbard, D.: Mathematical theory of radioimmunoassay. In: Principles of Competitive Protein-Binding Assays. Edited by: Odell, W.D., Daughadey, W.H., 158-203. J.B. Lippincot, Philadelphia, 1971.

16. Gabriel, O: Analytical disc gel electrophoresis. In: Methods in Enzymology. Vol. 22. Edited by: Jakoby, W.B., 565-577. Academic Press, New York, 1971.

17. Grage, H., Narag, R., Damodaran, K.,Anaokar, S. and Savjani, G.: Rapid solid-phase assay for progesterone. Clin. Chem., 32: 1153 (1986).

18. Grant, J.K. and Reastall, G.H.: Clinical Biochemistry of Steroid Hormones. Elsevier, New York, 1983.

19. Griswold, W.R and Nelson, D.P.: A rapid method for the determination of antibody affinity. Immunol. Lett., 7: 229-232 (1984).

20. Hillbrand, F.W. and Elsaesser, F.: Concentrations of progesterone in the backfat of pigs during the oestrus and after ovariectomy. J. Reprod. Fert., 69: 73-80 (1983).

21. Hiepe, F., Schwarz, G. and Hiepe, T.: Obtaining antibodies from the yolk of eggs of immunized hens. Monatsch. Vet., 43: 19-20 (1988).

22. IAEA: Laboratory Training Manual of Radioimmunoassay in Animal Reproduction. Technical Reports Series No. 233. International Atomic Energy Agency, Vienna, Austria, 1984.

23. Jensenius, J.C., Andersen, I., Hau, J., Crone, M. and Koch, C.: Eggs: Conveniently packaged antibodies, methods for purification of yolk IgG. J. Immunol. Methods, 46: 63-68 (1981).

24. King, G.J., Walton, J.S. and Bellissimo, D.J.: Anestrus in pigs: Confirmation by a solid-phase RIA for progesterone and subsequent response to treatment. Can. vet. J., 26: 2-6 (1985).

25. MacDonnel, H.F., Mullins, S. and Gordon, A.C.: Radioimmunoassay and enzymeimmunoassay of plasma progesterone as monitors of progesterone sponge treatment in ewes. Thmiogenology, 30: 303-321 (1988).

26. Malkinson, M.: The transmission of passive immunity to Escherichia coli from mother in the domestic fowl (Gallus domesticus). Immunology, 9: 311-317 (1965).

27. Marcus, G.J. and Hackett, A.J.: Use of enzyme-linked immunosorbent assay for measurement of bovine serum and milk progesterone without extraction. J. Dairy Sci., 69: 818-824 (1986).

28. Meisterling, E.M. and Dailey, R.A.: Use of concentration of progesterone and estradiol-17b in milk in monitoring postpartum ovarian function in dairy cows. J. Dairy Sci., 70: 2154-2161 (1987).

29. Mueller, A.P. and Wolfe, H.R.: Precipitin production following masive injection of BSA in adult chickens. Int. Arch. Allergy, 19: 321-325 (1961).

30. Munro, C. and Stabenfeldt, G.: Development of a microtitre plate enzyme immunoassay for the determination of progesterone. J. Endocrinol., 101: 41-49 (1984).

31. Naegele, W. and Drahovsky, M.: Production of steroid antisera. In: Radioimmunoassay of Steroid Hormones. 2nd ed. Edited by: Gupta, D., 55-72. Verlag Chemie, Weinheim, Germany, 1980.

32. Nisonoff, A.: Introduction to Molecular Immunology. Sinahuer Associates, Sunderland, Massachusetts, 1984.

33. Parker, W.CH.: Radioimmunoassay of Biologically Active Compounds. Prentice-Hall, Englewood Cliffs, New Jersey, 1976.

34. Patterson, R., Youngner, J.S., Weigle, W.O. and Dixon, F.J.: Antibody production and transfer to egg yolk in chickens. J. ImmunoL, 89: 272-278 (1962).

35. Pilo, A., Zucchelli, G.C., Chiesa, M.R., Franceschetti, F. and Albertini, A.: Improvement in the quality of estradiol, progesterone and testosterone RIAs-evaluation of the between kit and within kit contributes to the overall variability. Clin. Chem., 32: 1183 (1986).

36. Polson, A., Wechmar van, B.M. and Regenmortel van, H.V.: Isolation of viral IgY antibodies from yolks of immunized hens. Immunol. Commun., 9: 475-494 (1980).

37. Polson, A., Wechmar van, B.M. and Fazakerley, G.: Antibodies to proteins from yolk of immunized hens. Immunol. Commun., 9: 495-514 (1980).

38. Powrie, W.D.: Chemistry of egg and egg products. In: Egg Science and Technology. 2nd ed. Edited by: Stadelman, W.J., Cotterill, O.J., 65-91. AVI, Wesport, Connecticut, 1977.

39. Reimers, T.J., McCann, J.P. and Cowan, R.G.: Effects of storage times and temperature on T3, T4, LH, prolactin, insulin, cortisol and progesterone concentrations in blood samples from cows. J. Anim. Sci., 57: 683-691 (1983).

40. Runnebaum, B.: Quantitative determination of progesterone by radioimmunoassay. In: Radioimmunoassay of Steroid Hormones. 2nd ed. Edited by: Gupta, D., 143-151. Verlag Chemie, Weinheim, Germany, 1980.

41. Sauer, M.J., Foulkes, J.A. and O'Neill, P.M.: Use of microtitre plate EIA for direct determination of progesterone in whole milk: Application of heterologous systems for improved sensitivity. Br. vet. J., 138: 522-532 (1982).

42. Sauer, M.J., Foulkes, J.A., Worsfold, A. and Morris, B.A.: Use of progesterone 11-glucuronide-alkaline phosphatase conjugate in a sensitive microtitre-plate enzymeimmunoassay of progesterone in milk and its application to pregnancy testing in dairy cattle. J. Reprod. Fert., 76: 375-391 (1986).

43. Spearow, J.L. and Trost, B.A.: Development of a sensitive enzyme linked immunosorbent assay for cattle, sheep, rat and mouse luteinizing hormone. Biol. Reprod., 37: 595-605 (1987).

44. Srikandakumar, A,, Ingraham, R.H., Ellsworth, M., Archbald, L.F., Liao, A. and Godke, R.A.: A comparison of a solid phase, no-extraction radioimmunoassay for progesterone with an extraction assay for monitoring luteal function in the mare, bitch and cow. Theriogenology, 26: 779-793 (1986).

45. Staigmiller, R.B., Short, R.E., Bellows, R.A. and Carr, J.B.: Effect of nutrition on response to exogenous FSH in beef cattle. J. Anim. Sci., 48: 1182-1190 (1979).

46. Tho, P.T., Scholer, J., Phung van, B., Harp, R.J. and McDonough, PJ.: Radioimmunoassay of progesterone in saliva: A simplified technique using 125 I-radioligand. Fert. Steril., 43: 410-416 (1985).

47. Thorneycroft, I.H. and Stone, C.S.: Radioimmunoassay of serum progesterone in women receiving oral contraceptive steroids. Contraception, 5: 129-146 (1972).

48. Tizard, I.: Inmunología Veterinaria. 3a ed. Interamericana, México, D.F., 1989.

49. Wang, H.X., Stott, R.A., Thorpe, G.H.G., Kricka, L.J., Holder, G. and Rudd, B.T.: An enhanced chemiluminescence immunoassay for serum progesterone. Steroids, 44: 3294-3303 (1984).

50. Warburg, O. und Christian, W.: Isolierung und Kristallisation des Gärunsferments Enolase. Biochemische, 310: 384-421 (1941).

51. Wiel van de, D.F.M. and Koops, W.: Development and validation of an enzyme immunoassay for progesterone in bovine milk blood plasma. Anim. Reprod. Sci., 10: 201-213 (1986).

52. Wimpy, T.H., Chang, C.F., Estergreen, V.L. and Hillers, J.K.: Milk progesterone enzyme immunoassay: Modifications and a field trial for pregnancy detection in dairy cows. J. Dairy Sci., 69: 1115-1121 (1985).