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1994, Número 2

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Vet Mex 1994; 25 (2)


Producción de anticuerpos antiprogesterona a partir de la yema de huevo de gallinas y del suero sanguíneo de conejos, para ser utilizados en radioinmunoanálisis

Montes PRC, Murcia MC, Zarco QL

Idioma: Español/Inglés
Referencias bibliográficas: 52
Paginas: 117-125
Archivo PDF: 1073.15 Kb.


RESUMEN

Se indujo la producción de anticuerpos contra progesterona en gallinas Leghorn y conejos Nueva Zelanda, con el propósito de utilizarlos para establecer un Radioinmunoanálisis (RIA) para determinar progesterona. La extracción de anticuerpos de la yema del huevo de las gallinas inmunizadas se realizó con 2-propanolyacetona. Los anticuerpos del suero sanguíneo de conejo se extrajeron con solución de sulfato de amonio. Los anticuerpos de la yema del huevo mostraron elevada afinidad y especificidad hacia la progesterona. La sensibilidad del RIA establecido con estos anticuerpos fue de 0.27 ng/ml; los coeficientes de variabilidad intraensayo e interensayo fueron de 19.6 y 22.2%, respectivamente. Hubo una correlación de 0.94 entre los valores de progesterona determinados con un estuche comercial y los establecidos con los anticuerpos de la yema. Los anticuerpos obtenidos del suero de conejo también tuvieron elevada afinidad y especificidad hacia a la progesterona La sensibilidad de RIA que utilizó este anticuerpo fue de 0.23 ng/ml, el coeficiente de variabilidad intraensayo de 14.7% y el interensayo de 22.7%. Los valores de progesterona medidos con un estuche comercial y con el RIA establecido con los anticuerpos del conejo tuvieron una correlación de 0.96. Se concluye que la yema de huevo de gallinas inmunizadas es una fuente práctica de anticuerpos antiprogesterona de calidad comparable a la de los anticuerpos obtenidos a partir de suero sanguíneo de conejos. La producción de anticuerpos antiprogesterona extraídos de la yema del huevo de gallinas inmunizadas, fue 2.5 veces mayor que la obtenida del suero sanguíneo de conejos inmunizados contra el mismo antigeno.


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