medigraphic.com
ISSN 1870-199X (Print)
Órgano oficial de la Facultad de Odontología, UNAM

2010, Number 1

<< Back Next >>

Rev Odont Mex 2010; 14 (1)

Isolation and partial characterization of pulp stem cells

Magallanes FM, Carmona RB, Álvarez PMA

Language: Spanish
References: 21
Page: 15-20
PDF size: 243.41 Kb.


ABSTRACT

There is an increasing interest in the utility of dental pulp stem cells (DPSCs) for dental tissue regeneration. The purposes of this study were to isolate, to characterize and to lead cellular differentiation of stem cells from human dental pulp from extracted premolars of young patients. The stem cells isolated in cellular cultures generated clonogenic cultures after 5 weeks, presenting elongated and flat cells. This cells expressed mesenchymal stem cell markers (STRO-1 and CD-44) as shown by immunocytochemistry essay and they could differentiate in vitro into osteoblastic-like lineage. This differentiation was evaluated by RT-PCR and by immunocytochemistry and the results revealed that bone sialoprotein and osteopontin, two very important extracellular matrix protein, were expressed in the induction cultures and it also confirms the osteoblastic-like phenotype. In conclusion, cells from dental pulp with stem cell characteristics were isolated and the present study suggests that stem cells from human dental pulp may potentially be used as a cellular therapy in the tissue engineering area.


REFERENCES

  1. Krebsbach P, Gehron P. Dental and skeletal stem cells: Potential cellular therapeutic for craniofacial regeneration. Journal of Dental Education 2002; 66(6): 766-772.

  2. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A. Stem cell properties of human dental pulp stem cells. J Dent Res 2002; 81(8): 531-535.

  3. Gronthos S, Mankani M, Brahim J. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. J Cell Biology 2000; 97(25): 13625-13630.

  4. Batouli S, Miura M, Brahim J. Comparison of stem- cell- mediated osteogenesis and dentinogenesis. J Dent Res 2003; 82(12): 976-981.

  5. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Gehron RP, Shi S. SHED: Stem cells from human exfoliated deciduous teeth. PNAS 2003; 100: 5807-5812.

  6. Chen SC, Marino V, Gronthos S, Bartold PM. Location of putative stem cells in human periodontal ligament. J Periodont Res 2006; 41: 547–553.

  7. Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofacial Res 2005; 8: 191-199.

  8. Seo B, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Gehron-Robey P, Wang C, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004; 364: 149–55.

  9. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, DenBesten AP, Gehron RP, Shi S. Stem cell properties of human dental pulp stem cells. J Dent Res 2002; 81: 531-535.

  10. Risbud M, Shapiro I. Stem cell craniofacial and dental tissue engineering. Orthod Craniofacial Res 8: 2005: 54-59.

  11. Shi S, Robey P, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. J Bone Miner Res 2001; 29(6): 532-539.

  12. Gronthod S, Zannettino A, Graves S. Differential cell surface expression of the STRO-1 and alkaline phosphatase antigens on discrete developmental stages in primary cultures of human bone cell. J Bone Miner Res 1999; 14(1): 47-56.

  13. Stewart K, Walsh S, Screen A. Further characterization of cells expressing STRO-1 in cultures of adult human bone marrow stromal cell. J. Bone Miner Res 1999; 14(8): 1345-1356.

  14. Bianco P, Riminucci M, Gronthos S, Gehron-Robey P. Bone marrow stromal stem cells: Nature, biology, and potential applications. Stem Cells 2001; 19:180-192.

  15. Liu H, Gronthos S, SHI S. Dental pulp stem cells. Methods in Enzymology 2006; 419: 99-113.

  16. Ganns B, Him RH, Sodek J. Bone sialoprotein. Crit Rev Oral Biol Med 1999; 10: 79-98.

  17. Hunter GK, Goldber HA. Nucleation of hydroxyapatite by bone sialoprotein. Proc Natl Acad Sci USA 1993; 90: 8562-8565.

  18. Ramakrishnan PR, Lin WL, Sodej J, Cho MI. Synthesis of noncollagenous extracellular matrix proteins during development of mineralized nodules by rat periodontal ligament cells in vitro. Calcif Tissue Int 1995; 57: 52-59.

  19. Giachelli CM, Steitz S. Osteopontin a versatile regulator of inflammation and biomineralization. Matrix Biology 2000; 19: 615-622.

  20. Iohara K, Nakashima M, Ito M, Ishikawa M, Nakasima A, Akamine A. Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2. J Dent Res 2004; 83: 590-595.

  21. National Institutes of Health. Stem cell information. Stem cell basics. http://stemcells.nih.gov/info/basics




2020     |     www.medigraphic.com