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Coordinación General de Investigación de la Facultad de Salud Pública y Nutrición y la Dirección General de Sistemas e Informática de la Universidad Autónoma de Nuevo León

2009, Number 2

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Rev Salud Publica Nutr 2009; 10 (2)

Detección de escherichia coli o157:h7 en carne fresca de res mediante pcr múltiplex

Treviño LRA, Mata TV, Espinoza MA, Martínez VIO, Morales LA, Alvarez OG, Gallegos RMA

Language: Spanish
References: 22
Page:
PDF size: 148.79 Kb.


ABSTRACT

In the present study were compared the traditional microbiological method and two tests of multiplex PCR for the detection of E. coli O157:H7 in fresh beef meat. A significant difference was obtained between PCR multiplex and microbiological isolation in inoculated samples; in addition, in multiplex PCR was obtained a higher percentage of sensitivity, precision relative and Kappa index using the Ecm+n broth in comparison to the CST+ccv in the enrichment. In 40 samples of meat cuts American type were detected two positive samples (5%) by Multiplex PCR, whereas by the microbiological method was not obtained the isolation in any sample.


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