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Órgano Oficial del Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz

2001, Number 5

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Salud Mental 2001; 24 (5)

Comparación del análisis inmunoenzimático y la cromatografía de líquidos de alta eficiencia (EMIT y HPLC) para la medición de carbamacepina en muestras séricas

Moren J, Jaimes O, Dorantes AM, Nieves E, López G, Belmont A, Heinze G

Language: Spanish
References: 11
Page: 12-16
PDF size: 170.88 Kb.


ABSTRACT

The sera of 40 patients treated with carbamazepine were analyzed by enzyme immunoassay (EMIT) and high-performance liquid chromatography (HPLC) and the results were compared. The chromatographic method includes carbamazepine extraction with methylene chloride and its chromatographic separation in a Nova Pak C18 column by using a mixture of 25% v/v acetonitrile in 2O pH 5.6. Detection was made in an absorbance detector at 215 nm. Correlation between these two methods was 0.93. Confidence bands for predicting HPLC according to the EMIT with Working- Hotelling criterium showed 1.1, 0.5 and 1.6 μg/ml maximun error at low, medium and high ranges, respectively with a p=0.05 probability. The equation expressing the behavior of carbamazepine measurement by HPLC in function of EMIT is as follows: HPLC=0.824 x EMIT + 0.777 Afterwards, the chromatographic method was used for determining the serum predosis levels of carbamacepine and 10, 11-epoxi-carbamacepine in five epileptic patients that received an oral dosis of 200 mg of carbamacepine every 8 hrs as the only anticonvulsivant for controlling their crises.


REFERENCES

  1. ADAMS RF, VANDERMARK FL: Simultaneous highpressure liquid chromatographic determination of some anticonvulsants in serum. Clin Chem, 22:25-31, 1976.

  2. BREYER U: Rapid and accurate determination of the level of carbamazepine in serum by ultraviolet reflectance photometry on thin-layer chromatograms. J Chromatogr, 108:370, 1975.

  3. CHAN K: Simultaneous determination of carbamazepine and its epoxide metabolite in plasma and urine by high performance liquid chromatography. J Chromatography Biomedical Applications, 342:341-347, 1985.

  4. DE VANE CL: Intraclass comparisons of antidepressants and mood stabilizers. En: Fundamentals of Monitoring Psychoactive Drug Therapy. Williams & Wilkins ed. página 128, Baltimore, 1990.

  5. BASTIANI RJ, PHILLIPS RC, SCHNEIDER RS, ULLMAN EF: Homogeneous immunochemical drug assays. Am J Med Technol, 39:211, 1973.

  6. Goodman GA: Fármacos eficaces para el tratamiento de las epilepsias. En: Las Bases Farmacológicas de la Terapéutica. Novena edición, Vol. 1 Mc Graw-Hill Interamericana, Pág. 504- 505, México, 1996.

  7. HARRISON: Carbamacepina y ácido valproico. En: Principios de Medicina Interna. Décimatercera ed. Vol. II. Mc Graw- Hill Interamericana, pag. 2780, España, 1994.

  8. KUPFERBERG HJ: GLC determination of carbamazepine in plasma. J Pharm Sci, 61: 284-286, 1972.

  9. MATAR KM, NICHOLLS PJ, AL-HASSAN MI, TEKLE A: Rapid micromethod for simultaneous measurement of oxcarbamazepine and its active metabolite in plasma by highperformance liquid chromatography. J Clinical Pharmaty Therapeutics, 20:229-234, 1995.

  10. ROGER JD, ROGERS JRG, SOV A: Simultaneous determination of carbamazepine (tegretol) and other anticonvulsants in human plasma by gas-liquid chromatography. Clin Chem, 19:590, 1973.

  11. VANDEMARK FL, ADAMS RF: Ultramicro gas chromatographic analysis for anticonvulsants. With use of a nitrogen selective detector. Clin Chem, 22:62, 1976.




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