medigraphic.com
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

1999, Number 1

<< Back Next >>

Rev Biomed 1999; 10 (1)

Evaluation of an ultramicroELISA for the detection of infection by the respiratory syncytial virus.

Miguez-Lorenzo J, Tejero-Suárez Y, Savón-Valdés C, Laferté-Serrano J, Rodríguez-Calle RM, Goyenechea-Hernández A, González-Muñoz G, Hernández- Espinosa B

Language: Spanish
References: 26
Page: 7-15
PDF size: 39.35 Kb.


ABSTRACT

Objective. The present study was aimed at evaluating antibody ultramicroELISA for rapid detection of IgG antibodies to Respiratory Syncytial virus in single and pair sera. Materials and methods. 1567 serum samples were evaluated by both complement fixation (FC) and ultramicroELISA (UME) techniques: 619 single serum samples from children under 15 years and 474 pair serum samples from the national surveillance seroepidemiological program for acute Respiratory Diseases. In the double antibody UME technique, a specific monoclonal antibody against the F protein of RSV was used. Results.When comparing the ultramicroELISA with the FC technique, values obtained for single serum samples were: sensitivity was 98.9 %, specificity 56.05% and agreement 63.9 %. And for the pair serum samples sensitivity was 92.7 %, while specificity and agreement increased in a 79.3 % and a 80.1 % respectively. Conclusions. The use of this monoclonal antibody allowed the inclusion of rough antigen preparation in stead of purified fractions, leading to a remarkable decrease in the reactivity obtained with antigen control. These results back up the use of this method as a complementary tool in seroepidemiological studies carried out on a large scale and for diagnostic purposes.


REFERENCES

  1. 1.- Goldman DA. Nosocomial viral infections: recent developments and new strategies. Europ J Microbiol Infect Dis 1989; 8:75-81.

  2. 2.- Englund JA, Sullivan CJ, Jordan MC, Dehner LP,Vercelotti GM, Balfour HH. Respiratory Syncytial virus infections in immunocompromised adults. Ann Intern Med 1988; 109:203-8.

  3. 3.- Parrot RH, Kim HW, Brant CD, Beem MO, Richardson L, Gerin JL, Chanock RM. Respiratory syncytial virus. Diagnostic Procedures for Viral, Rickettsial and Chlamidial Infections, 5ta ed, Washington D C: American Public Assoc Inc.; 1979. p. 695-708.

  4. 4.- Meurman O, Russkanen O, Sarkkinen H, Hanninen P,Halonen P. Immunoglobulin class-specif antibody response in respiratory syncytial virus infection measured by enzyme immunoassay. J Med Virol 1984; 14: 67-72.

  5. 5.- Jomarrón L, Otero A, Alvarez M, Marrero M, Laferté J, Roges G . Design of standard curve for the measurement of IgG antibodies against human cytomegalovirus in a single serum dilution by ultramicro-ELISA. Biotec Aplic 1993; 10:200-3.

  6. 6.- Laferté J, Savón C, Goyenechea A, Vázquez S, Otero A, Tejero Y, et al. Estimación del título de anticuerpos IgG a adenovirus utilizando una curva patrón y un ensayo de ultramicroELISA indirecto. Rev Cub Med Trop 1996; 48:102-8.

  7. 7.- Ribas M A, Otero A, Alvarez M, Marrero M, Vázquez S. Diseño de una curva patrón para la detección de inmunoglobulina G humana contra herpes simple a partir de una dilución única del suero. Rev Cub Med Trop 1994; 46:32-6.

  8. 8.- Rivas M, Laferté J, Alberti A, Rosario D, González G. Algunas aplicaciones del Sistema Ultramicroanalítico al diagnóstico de las enfermedades infecciosas. Rev Cub Med Trop 1992; 44:226-7.

  9. 9.- Lowry OR, Rosebrowgh N J, Randall R J. Protein measurement with the Folin- phenol reagent. J Biol Chem 1951; 193:265-75.

  10. 10.- Palmer DF, Whales C. Complement Fixation Test.En: Rose NR, Friedman H, Fahey JL eds. Manual of Clinical Laboratory Immnunology. New York: Am Soc Microbiol; 1986. p. 57-66.

  11. 11.- Gardner PS, McQuillin J. Application of immunofluorescent antibody technique in rapid diagnosis of respiratory syncytial virus infection. Br Med J 1968; 3:340-3.

  12. 12.- Savón C, Laferté J, Goyenechea A, Valdivia A, Morier L, Tejero Y. Normalización de un ensayo ultramicroELISA para la detección de anticuerpos IgG al Virus Sincitial Respiratorio. Rev Cub Med Trop 1996; 48 (en prensa).

  13. 13.- Griner PF, Mayewski R J, Musilin A I, Green Land P. Selection and interpretation of diagnostic tests and procedures. Principles and Aplications. Ann Intern Med 1981; 94:553-600.

  14. 14.- Meddens JMM, Herbrink P, Lindeman J, Willemien C, Dijk V. Serodiagnosis of Respiratory Syncytial Virus (RSV) Infection in Children as Measured by detection of RSV-Specific Inmunoglobulins G, M, and A with Enzyme- Linked Inmunosorbent Assay. J Clin Microbiol, 1990; 28:152-5.

  15. 15.- Grandien M, Petterson CA, Gadner PS, Linde A, Stanton A. Rapid viral diagnisis of acude respiratory assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretion. J Clin Microbiol 1985; 22:757-60.

  16. 16.- Pilaipon P, Supreeda H, Rachaneeporn T, Chantapog W. Serological response to respiratory Syncitial virus infection in pediatric patients with a comparison to immunofluoscence and isolation. J Allerg Immunol 1995; 13:37-41.

  17. 17.- Ali A, Ali R. Enzyme-linked immunosorbent assay for anti-DNA antibodies using fluorogenic and colorigenic substrates. J Immunol Methods 1983; 56:341-46.

  18. 18.- Hildreth SW, Beaty BJ, Meegan JM, Frazier CL,Shope RE. Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems. J Clin Microbiol 1982; 15:879-84.

  19. 19.- Yolken RH, Stopa PJ. Enzyme-linked fluorescence assay: ultrasensitive solid-phase assay for detection of human rotavirus. J Clin Microbiol 1979; 10:317-21.

  20. 20.- Van Enk RA, James KK, Thompson KD. Evaluation of three commercial enzyme immunoassays for Toxoplasma and Cytomegalovirus Antibodies. J Clin Pathol 1991; 95:428-34.

  21. 21.- Hornsth A, Fries B, Kraslinikof PA. Detection of respiratory syncitial virus in nasopharyngeal secretion by biotin-aviden ELISA more sensitive than the fluorescent antibody techniques. J Med Virol 1986 ; 18:113-7.

  22. 22.- World Health Organization. Rapid laboratory techniques for the diagnosis of viral infections. Report of WHO scientific group. WHO Tech Rep Ser; 1981. p. 661.

  23. 23.- Edward J, Shillito E. Decline in specificity of the ELISA due to storage of serum, and its recovery by adsorption with kaolin. J Virol Methods 1982; 4:241-8.

  24. 24.- Katz S, Jordan WS, Badger J, Dingle J. Studies of complement-fixing and neutralizing antibodies against certain adenovirus. J Immunol 1957; 78:118-21.

  25. 25.- Richardson LA, Yolken RH, Belshe RB, Camango E,Kim HW, Chamock RM. Enzyme-linked. immunosorben assay for measurement of serological responce to Respiratory Syncytial virus infection. Infect Immunity 1978; 20:660-4.

  26. 26.- Boteler WL. Enzyme linked immunosorbent assay for detection of measles antibody. J Clin Microbiol 1983; 17:814-8.




2020     |     www.medigraphic.com