Diagnostic value of Helicobacter pylory antibodies in patients referred to the digestive endoscopy service at the Hospital San Vicente de Paul, Costa Rica.

Eugenia M. Quintana-Guzmán; Pilar Salas-Chaves; Rosario Achí-Araya; Henry Davidovich-Rose; Karl Schosinsky-Nevermann

2002, Number 1

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Rev Biomed 2002; 13 (1)

**Diagnostic value of Helicobacter pylory antibodies in patients referred to the digestive endoscopy service at the Hospital San Vicente de Paul, Costa Rica.**

Quintana-Guzmán EM, Salas-Chaves P, Achí-Araya R, Davidovich-Rose H, Schosinsky-Nevermann K

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Language: Spanish
References: 27
Page: 15-23
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ABSTRACT

Introduction. Antibodies titers against H. pylori antigens were determined in 78 patients referred to the Digestive Endoscopy Service of the San Vicente de Paul Hospital, Costa Rica. The assay was performed by enzyme immunoassay (ELISA), using commercial and non comercial methods with costarrican bacterial antigens and antigens prepared in Sweden, with the purpose of determining the diagnostic sensitivity and specificity of IgG, IgM and IgA inmunoglobulines. Material and methods. The commercial ELISA method was performed according to instructions and the two ELISA methods were prepared in the laboratory using no commercial antigens, one from Costa Rica and the other prepared in Sweden. Results. H. pylori infection was present in 83% of studied patients. The determination of IgG anti H. pylori by commercial ELISA method presents the highest diagnostic sensitivity (76%) and the non commercial ELISA using costarrican antigen shows the lowest diagnostic sensitivity (57%) with the highest diagnostic specificity (92%). The non commercial ELISA method more specific was the one that uses costarrican bacterial antigen, but the antigen prepared in Sweden had the highest sensitivity (67%). Discussion. The use of antigens prepared of local strains permits the detection of the presence of H. pylori with greater confidence as compared to antigens prepared with strains from other regions,because elevated serologic tests are high suggestive of the presence of the bacteria in the gastric mucous. The anti H. pylori inmunoglobulin with the highest diagnostic sensitivity and specificity was the IgG, but a histologic study of gastric biopsy instead of using the serologic test alone is recommended for the diagnosis of H. pylori.

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