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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2009, Number 2

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Rev Mex Patol Clin Med Lab 2009; 56 (2)

Immunosuppressive effect in human lymphocytes of Aeromonas hydrophila biotype 296 isolated from oysters in Mexico City

Vázquez-López R, Solano GS, Fernández PAM, Barragán JR, Ruy-Díaz J, Barrientos FT

Language: Spanish
References: 35
Page: 117-124
PDF size: 125.70 Kb.


ABSTRACT

At the College of Medicine in the Anáhuac Universidad, it was isolated Aeromonas hydrophila biotype 296 oysters obtained from Mexico City restaurants. It was identified by a confidence interval of 0.9958. This finding indicates that it is very important to investigate about the frequency of appearance of Aeromonas in this kind of food. Isolation of Aeromonas implies a potential health risk, since this bacterium is associated with serious enteritis cases. At the College of Medicine of the National Autonomous University of Mexico, pure colonies of that mentioned bacteria were seeded in brain heart infusion (BHI) broth; the supernatant obtained was used to perform the blastoid proliferation assay in order to determine its effect over human lymphocytes from peripheral blood. The lymphocytes were incubated in the presence of two different concentrations of proteins obtained from the supernatant (20 and 50 µg). Aeromonas hydrophila produces, in vitro, a powerful dose-dependent immunosuppressive effect (p ‹ 0.001). These observations suggest that the immunosuppressive effect might be a new pathogenicity mechanism produced by Aeromonas hydrophila. If this phenomenon occurs in vivo, it could give very important elements in order to get a better knowledge about the physiopathology of diverse diseases produced by this bacterium. This is to say, if an immunosuppressive effect in the infected tissue happened, it would be induced a local inhibition of lymphocyte proliferation, needed during the immune response induction.


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