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2009, Number 2

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Bioquimia 2009; 34 (2)

Interleukin-1β increase the SRP72 autoantigen expression

Arana-Argáez VE, Martínez-García EA, Martín-Márquez BT, Muñoz-Valle JF, García-Iglesias T, Delgado-Rizo V, Vázquez-Del MM

Language: Spanish
References: 19
Page: 77-82
PDF size: 129.40 Kb.


ABSTRACT

In the search of phosphorilated proteins recognized by the immune system as autoantigens, was identified the signal recognition particle (SRP) complex, in particular the subunit of 72 kDa (SRP72) as an autoantigen phosphorilated in serine residues seen in patients with dermatomyositis. One of the possible pathways involved in this postranslation modification is the MAPK pathway. The aim of this work was to identify if IL-1β is able to increase the SRP72 autoantigen expression and the kinase signaling pathway involved in the phosphorylation of SRP72 by IL-1β. Our preliminary results show that SRP72 activation was seen 90-120 min after addition of IL-1β (100 pg/mL). Using MAPK inhibitors we found a diminished expression of SRP72 protein 90-120 minutes after the addition of the inhibitor. We postulate that SRP72 protein is a phosphoprotein that is able to modify its expression by MAPK inhibitors.


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