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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2003, Number 3

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Rev Biomed 2003; 14 (3)

Analysis of HLA DQA1 and DQB1 alleles and glycated hemoglobin in healthy Mexican mestizo individuals from families with type 1 diabetes mellitus

Hermosillo-Bañuelos RM, Ramos C, Perea FJ, Rivas F, Casas-Castañeda M, Camacho AL, Sánchez YJ, Ibarra B

Language: English
References: 23
Page: 125-130
PDF size: 36.51 Kb.


ABSTRACT

Introduction. Variations in hemoglobin (HbA1) levels in healthy individuals are attributable to complex individual biological differences. Specific HLA DQ genes are known to predispose subjects to the development of type 1 diabetes mellitus (DM1).
Material and methods. HLA DQA1 and DQB1 genes and HbA1 heritability were investigated in healthy individuals from DM1 and non-DM1 Mexican families. HbA1 levels were determined by ionexchange chromatography and HLA class II alleles were typed by PCR, in 77 healthy persons from 18 families with at least one member suffering from DM1 (DM1R group) and in 96 healthy individuals from 18 families with no known history of DM, who constituted the control group.
Results. There were no significant inter-group differences in HbA1 levels. Mean HbA1 levels were correlated between parents and offspring, and the heritability estimate was 53% based on a regression model. DQA1 and DQB1 typing revealed inter-group differences, with an increase in the susceptibility allele, DQB1 *0201, in the DM1R group relative to the control group (17.24% vs 3.23%, respectively; p ‹ 0.05), as expected. HbA1 levels did not correlate with HLA DQA1 or DQB1 polymorphisms.
Discussion. The present data suggest that 53% of HbA1 variability is explicable by genetic factors, which do not depend on DQA1 or DQB1 polymorphisms.


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