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2005, Number 1

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Rev Mex Patol Clin Med Lab 2005; 52 (1)

Quick detection of platelet concentrates contaminated by bacterias

JA Vázquez, A Flores Aréchiga, R Cázares Taméz, César Retes, G Prado Bernal, Jorge Canizales Oviedo

Language: Spanish
References: 18
Page: 22-26
PDF size: 42.54 Kb.


ABSTRACT

Introduction: When transfusing a polluted hemocomponent with bacterias you can cause a bacterial sepsis. The temperature of conservation of the Platelet Concentrates (PC) of 20-24°C it could favor the bacterial growth. Objective: To implement quick, easy and economic method for the identification of platelet concentrates contaminated by bacterias. Material and methods: A total of 2,804 units was processed, 2132 PC entered to the study, to which are determined glucose and pH using reagent strips, with glucose ≤ of 200 and/or pH ≤ of 6, they were considered positive and they were sent to bacteriology for culture in two enriched means, agar blood and thioglicolato broth, if this presented alteration, you proceeded to sow in agar blood, agar azida and EMB. Results: Of the 2,132, 26 were positive in the method of reagent strips, of these 26 correspondents to bacteriology, 2 were positive and it was determined in both that it was a Propionibacterium acnes; a PC had four days of storage (glucose 200, pH 6) and the other five days (glucose 200, pH 6.5). Conclusions: We could suggest for our hospital that before to the transfusion to do the method of reagent strip to the PC which have three or more days of storage.


REFERENCES

  1. Buchholz DH, Young VM, Friedman NR, Reilly JA, Mardiney MR Jr. Detection and quantification of bacteria in platelet products stored at ambient temperature. Transfusion 1973; 13: 268-275.

  2. Wrenn HE Speicher CE. Platelet concentrates: Sterility of 400 single units stored at room temperature. Transfus Med 1974; 14: 171-172.

  3. Blajchman MA, Ali AM, Richardson HL. Bacterial contamination of cellular blood components. Vox Sang 1994; 67 (suppl 3): 25-33.

  4. Yomtovain R, Lazarus HM, Goodnough LT et al. A prospective microbiologic surveillance program to detect and prevent transfusion of bacterially contaminated platelets. Transfusion 1993; 33: 902-909.

  5. Hogman CF. Adverse effects: Bacterial contamination (including shelf life): A brief review of bacterial contamination of blood components. Vox Sang 1996; 70 (suppl 3): 78-82.

  6. Gibson T, Norris W. Skin fragments removed by injection needles. Lancet 1958; 2: 983-985.

  7. BPAC discusses invalidation of test results, platelet usage. Blood Bank Week 1992; 9 (5): 1-3.

  8. Bertolini F, Murphy S. A multicenter evaluation of reproducibility of swirling in platelet concentrates. Biomedical Excellence for Safer Tranfusion (BEST) Working Party of the International Society of Blood Transfusion. Transfusion 1994; 34: 796-801.

  9. Mitchell TKM, Brecher ME. Approaches to the detection of bacterial contamination in cellular blood products. Transfus Med Rev 1999; 13: 132-144.

  10. McCarthy LR, Senne JE. Evaluation of acridine orange stain for detection of microorganisms in blood cultures. J Clin Microbiol 1980; 11: 281-285.

  11. Brecher me, Hogan JJ, Boothe G et al. Platelet bacterial contamination and the use of a chemiluminescence linked universal bacterial ribosomal RNA gene probe. Transfusion 1994; 34: 750-755.

  12. Brecher ME, Wong ECC, Chen SE et al. Vancomycin linked probes and microvolume fluorimetry for the rapid detection of Gram positive bacterial contamination in platelet products (abstract). Transfusion 1998; 38 (suppl): 106S.

  13. Feng P, Keasler SP, Hill WE. Direct identification of Yersina enterocolitica in blood by polymerase chain reaction amplification. Transfusion 1992; 32: 850-854.

  14. Vyas GN, Yang G, Murphy EL. Transfusion-related transmission diseases. Detection by polymerase chain reaction amplified genes of the microbial agents. Transfus Med Rev 1994; 8: 253-266.

  15. Burstain JM, Brecher ME, Workman K et al. Rapid identification of bacterially contaminated platelets using reagent strips: glucose and Ph analysis as markers of bacterial metabolism. Transfusion 1997; 37: 255-258.

  16. Werch JB, Paulette Mhawech et al. Detecting Bacteria in platelet concentrates by use of reagent strips. Transfusion 2002; 42: 1027-1031.

  17. Jakab E, Zbinden R, Gubler J et al. Severe infections caused by Propionibacterium acnes: An underestimated pathogen in late postoperative infections. Yale J Biol Med 1996; 69: 477-482.

  18. Bruneau C, Perez P, Chassaigne M, Allouch P et al. Efficacy of a new collection procedure for preventing bacterial contamination of whole-donations. Transfusion 2001; 41: 74-81.




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