Balderas PLMA, Vizcaíno MCV, Hernández HS, Vargas GC, Álvarez RF, García IT, Toro AS, Daneri NA

Language: Spanish
References: 25
Page: 327-335
PDF size: 412.60 Kb.

ABSTRACT

Background: Preeclampsia origin has no conclusive explanation. As part of its etiology it has been proposed immunologic disorders. This work explores several lymphocytes subsets and postulates possible mechanisms involved in a lost of immune tolerance in this entity.
Objective: To compare cellular populations of CD3⁺ CD56⁺, CD4⁺ CD25⁺, T lymphocytes (CD4⁺ and CD8⁺) and NK cells subsets in preeclamptic and pregnant healthy women.
Patients and methods: Through flow cytometry antibodies, peripheral blood mononuclear cells were obtained from both groups of patients. CD3⁺ CD56⁺, CD4⁺ CD25⁺, T lymphocytes (CD4⁺ and CD8⁺) and NK cells were identified. Mean and standard deviation, Student t test and Pearson correlation were calculated to analyze differences between groups and correlation between mean blood pressure and different lymphocytes subsets; p ‹ 0.05 was considered significant.
Results: CD3⁺ CD56⁺ cells percentage was lower in preeclamptic patients (2.7 vs 6.1%; p ‹ 0.002), CD4⁺ CD25⁺ cells percentage tend to be lower too (22.11 vs 33.86; p = NS). Mean blood pressure shown negative correlation with CD3⁺ CD56⁺ cells percentage (rp - 0.666; p = 0.001) and with CD25 on CD4⁺ T lymphocytes surface (rp - 0.526; p ‹ 0.025).
Conclusions: Based on the association between mean blood pressure and lymphocytes percentage for these two cellular subsets, data obtained suggest that CD3 CD56⁺ and CD4⁺ CD25⁺ cells play an important role in preeclampsia development.

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