Fernández-Molina C, Rodríguez-Preval N, Rodríguez-González I, Agnese-Latino M, Rivera-Tapia JA, Ayala-Rodríguez I

Language: Spanish
References: 20
Page: 358-362
PDF size: 170.97 Kb.

ABSTRACT

Objective. Mycoplasma genitalium has been associated with nongonococcal urethritis (NGU). Diagnosis by PCR has become the primary detection method for this organism. Thus, diagnosis by DNA amplification using the PCR technique should be utilized. Material and Methods. GMF/GMR and MgpF/MgpR primer pairs, complementary to the M. genitalium 16S rRNA and MgPa genes, respectively, were selected. Specificity and sensibility assays were conducted and clinical samples were studied. Results. The PCR with each primer pair was specific only for M. genitalium, and the sensibility was higher with the GMF/GMR primers. In the study of 34 clinical samples, 18,5% were positive for M. genitalium, with more positive samples when the MgpF/MgpR primers were used. Conclusions. DNA amplification by PCR should be applied in clinical practice to the diagnosis of M. genitalium in patients with NGU should using.

REFERENCES

1. Jensen JS. Mycoplasma genitalium: an etiological agent of urethritis and other sexually transmitted diseases. J Eur Acad Dermatol Venerol 2004;18:1-11.

2. Wikstrom A, Jensen JS. Mycoplasma genitalium: a common cause of persistent urethritis among men treated with doxycycline. Sex Transm Infect 2006;82(4):276-279.

3. Jurstrand M, Jensen JS, Fredlund H, Falk L, Molling P. Detection of Mycoplasma genitalium in urogenital specimens by real-time PCR and by conventional PCR assay. J Med Microbiol 2005;54:23-29. PCR assay. J Med Microbiol 2005;54:23-29.

4. Yoshida T, Maeda S, Deguchi T, MiyazawaT, Ishiko H. Rapid detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms in genitourinary samples by PCR-microtiter plate hibridization assay. J Clin Microbiol 2003;41(5):1850-1855.

5. Hamasuna R, Osada Y, Jensen JS. Isolation of Mycoplasma genitalium from first-void urine specimens by coculture with vero cells. J Clin Microbiol 2007;45(3):847-850.

6. Hardick J, Giles J. Performance of the gen-probe transmission- mediated amplification research assay compared to that of a multitarget real-time PCR for Mycoplasma genitalim detection. J Clin Microbiol 2006;44(4): 1236-1240.

7. Jensen J, Borre M, Dohn B. Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene. J Clin Microbiol 2003;41(1):261-266.

8. Jensen JS, Bjornelius E, Dohn B, Lidbrink P. Use of TaqMan 5’nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J Clin Microbiol 2004;42:683-692.

9. Fernandez CM, Alvarez K, Muy L, Martinez M. Detection using molecular biology techniques of Mycoplasma hominis and Ureaplasma urealyticum in urogenital samples. Rev Argent Microbiol 1998;30(2):53-58.

10. Fernández CM, Latino MA, Zamora YM, Pellecchia M, Neve V, Llanes R, et al. Desarrollo de un método de PCR-múltiple para la identificación de Ureaplasma parvum y Ureaplasma urealyticum. Rev Argent Microbiol 2003;35:3.

11. Taylor-Robinson D, Ainsworth JG, McCormack WM. Genital mycoplasmas. In: Sexually transmitted diseases. Holmes KK, Sparling, PF, Mardh P-A, et al, eds. New York: McGraw-Hill, 2004:533-548.

12. Gubelin W, Martínez MA, Céspedes P, Fich F, Fuenzalida H, De la Parra R, et al. Aplicación de método molecular en la detección de M. genitalium en hombres y en mujeres embarazadas. Rev Chil Infect 2006;23(1):15-19.

13. Dan L, Wenyuan Z, Xiaojun Z, Xiping C, Shukui W, Zizheng W, et al.Molecular epidemiologic study of Mycoplasma genitalium infection in high risk populations of sexually transmitted diseases in China. Chin Med J 2000;113(11):1015-1018.

14. Anagrius C, Loré B, Jensen JS. Mycoplasma genitalium: prevalence, clinical significance, and transmission. Sex Transm Infect 2005;81:458-462.

15. Dolapci I, Tekeli A, Ozsan M, Yaman O, Ergin S, Elhan A. Detecting of Mycoplasma genitalium in male patients with urethritis symptoms in Turkey by plymerase chain reaction. Saudi Med J 2005;26(1):64-68.

16. Edberg A, Johansson E, Wikander E, Hoog A, Ahlqvist T, Jurstrand M,et al. A comparative study of three different PCR assays for detection of Mycoplasma genitalium in genital specimens from men and women. In: European Society of Clinical Microbiology and Infectious Diseases. 16th European Congress of Clinical Microbiology and Infectious Diseases; 2006 April 1-4 de; Nice, France.

17. Hardick J, Giles J. Performance of the gen-probe transmissionmediated amplification research assay compared to that of a multitarget real-time PCR for Mycoplasma genitalium detection. J Clin Microbiol 2006;44 (4):1236-1240.

18. Svenstrup HF, Jensen JS, Bjornelius E, Lidbrink P, Birkelund S, Gunna C. Development of a quantitative real-time PCR assay for detection of Mycoplasma genitalium. J Clin Microbiol 2005;43:3121-3128.

19. Baseman JB, Cagle M, Korte JE, Herrera C, Rasmussen WG, Baseman JG, et al. Diagnostic assessment of Mycoplasma genitalium in culturepositive women. J Clin Microbiol 2004;42(1):203-211.

20. Eastick K, Leeming JP, Caul EO, Horner PJ, Millar MR. A novel polymerase chain reaction assay to detect Mycoplasma genitalium. Mol Pathol 2003;56(1):25-28.