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2008, Number 2

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Vet Mex 2008; 39 (2)

Study on the osseous consolidation in rat by environmental scanning electron microscopy

Neri BR, Solís AL, Villegas CH

Language: English/Spanish
References: 30
Page: 187-198
PDF size: 469.44 Kb.


ABSTRACT

The environmental scanning electron microscope (ESEM) has been recently applied to orthopedics, for which a study was performed on the modes of operation of this electron microscope. Optimal conditions were determined to obtain the best topographic image and to perform chemical analysis by X-ray dispersion during the process of bone consolidation. Fifteen male Wistar rats were used, grouped in three per each experimental day. Tibial fracture was provoked and a Kirschner wire was positioned. Rats were euthanized 0 (control), 7, 14, 21 and 28 days after surgery. According to the results, the best bone image was observed with retrodispersed electrons under high vacuum. In the chemical element determination lower variation coeffi cients were obtained under low vacuum for carbon and calcium; for phosphorus, under high vacuum, and in environmental mode, for magnesium, sodium, potassium and iron. In the process of bone consolidation, calcium levels in cortical bone diminished compared to controls, while the calcifi ed bone callus was observed until day 14. Phosphorus concentration increased with time, in both cortical bone and bone callus. Magnesium presented a peak on day 14, and decreased drastically until day 28. Sodium rose continuously until day 28. The study of the chemical composition of bone by the ESEM is optimal in the environmental mode, since values of the elements present in lesser concentrations (1%) showed a lower variation coeffi cient. In bone consolidation process, calcium and phosphorus levels were found at a ratio of 2:1.


REFERENCES

  1. 1.Danilatos GD. Foundation of environmental scanning electron microscopy. Adv Electron Phys 1988; 71:109- 250.

  2. 2.Goldstein JI, Newbury DE, Echlin P, Joy DC, Roming AD, Lyman CE et al. Scanning electron microscopy and X-ray microanalysis. 2nd ed. New York: Plenum Press, 1992.

  3. 3.Hayat MA. Principles and techniques of scanning electron microscopy. Biological aplications. New York: Van Nostrand Reinhold Co, 1978.

  4. 4.Yamada N, Nagato M, Murakami S, Ikeuchi M, Oho E,Baba N et al. Preparation for observation on the fi ne structure of biological specimens by high resolution SEM. J Electron Micros 1983; 32:321-330.

  5. 5.Vázquez Nin G, Echeverría O. Introducción a la microscopía electrónica aplicada a las ciencias biológicas.México D F: Fondo de Cultura Económica, 2000.

  6. 6.Danilatos GD. Enviromental scanning electron microscopy and microanalysis. Mikrochim Acta 1994;114/115:143-155.

  7. 7.Tai SS, Tang XM. Manipulating biological samples for enviromental scanning electron microscopy observation. Scanning 2001; 23:267-272.

  8. 8.Danilatos GD. Introduction to the ESEM instrument.Microsc Res Tech 1993; 25:354-361.

  9. 9.Boyd AS, Seger O, Vannucci S, Langley M, Abraham JL.Exposición al mercurio y enfermedades cutáneas. J Am Acad Dermatol 2000; 43: 81-90.

  10. 10.Ligon JJ, Abraham JL, Boyd AS. Traumatic osmium tetroxide inoculation. J Am Acad Dermatol 2001;45:949-952.

  11. 11.Schwille PO, Schmiedl A, Hermann U, Fan J, Gottieb D, Manoharam M et al. Magnesium, citrate magnesium and magnesium-alkali citrate as modulators of calcium oxalate crystallization in urine: Observation in patients with recurrent idiopathic calcium urolithiasis. Urol Res 1999; 27:117-126.

  12. 12.McMenanin PG, Djano J, Welthall R. Griffi n BJ. Char-acterization of the macrophages associated with the tunica vasculosa lentis of the rat eye. Invest Ophthalmol Vis Sci 2002; 43:2076-2082.

  13. 13.Donald AM. The use of enviromental scanning electron microscopy for imaging wet and insulating materials.Nature Mater 2003; 2:511-516.

  14. 14.Griffi n BJ, Suvorova AA. Charge-related problems associated with X-ray microanalysis in the variable pressure scanning electron microscope at low pressures. Microsc Microanal 2003; 9:155-165.

  15. 15.Tang X, Joy DC. Quantitative measurements of charging in a gaseous environment. Scanning 2003; 25:194-200.

  16. 16.Timofeeff NM, Lowenstein KT, Blackburn HW. ESEMEDS: an improved technique for major element chemical analysis of fl uid inclusions. Chem Geol 2000; 164:171-182.

  17. 17.Reid AP, Oates K, Potes WTW. The effects of aluminum coating on elemental signals in X-ray microanalysis. Micros Res Tech 1993; 24:168-172.

  18. 18.Villegas CH, Mayon GJ, González MG, González JM,Ibarra MG, Murillo UA. Estudio de la osteoporosis y osteopenia por medio de radiología, densitometría, morfometría, microscopía de barrido en la tercera vértebra lumbar en una muestra de hombres y mujeres en la Ciudad de México. Ginecol Obstet Méx 1998; 66: 187-201.

  19. 19.Quintana-Armenta A, Ruiz-Rosano L, Valdez MR, Diez GP, Isoard AF, Herrero BB et al. Medición del contenido mineral óseo en las vértebras lumbares L2-L5 de ratas Long Evans hembras por absorciometría de rayos X de doble energía y por análisis químico. Acta Ortop Mex 2003; 17:168-172.

  20. 20.Diez GM, Chávez AD, Mercado CR, Domínguez HV,Torres MA, Lomelí MP et al. Modelo experimental de fracturas y consolidación ósea en ratas. Rev Mex Ortop Traum 2002; 16:113-117.

  21. 21.Mendoza-Barrera C, Meléndez LM, García LE, Hernández FC. Caracterización estructural y biointegración del sustituto óseo BioOsteo. Rev Mex Fís 2004; 50:19-23.

  22. 22.Rude RK. Magnesium defi ciency: a heterogeneous cause of disease in humans. J Bone Miner Res 1998; 13: 749-758.

  23. 23.Rude RK, Gruber EH, Wei YL, Frausto A, Mills GB.Magnesium defi ciency: Effect on bone and mineral metabolism in the mouse. Calcif Tissue Int 2003; 72:32-41.

  24. 24.Rude RK, Gruber EH. Magnesium defi ciency and osteoporosis: animal and human observations. J Nutr Biochem 2004; 15:710-716.

  25. 25.Lietz G, Avenell A, Robins SP. Short-term effects of dietary sodium intake on bone metabolism in postmenopausal women measured using urinary deoxypyridinoline excretion. Br J Nutr 1997; 78:73-82.

  26. 26.Lai-Ping E, Swaminathan R. Calcium metabolism and bone calcium content in normal and oophorectomized rats consuming various levels of saline for 12 months. J Nutr 1998; 128:633-639.

  27. 27.Chan AY, Poon P, Chan ELP, Fung SLM, Swaminathan R. The effect of high sodium intake on bone mineral content in rats fed normal calcium or low calcium diet.

  28. 28.Osteop Int 1992; 3:341-344.Massey LK, Whiting SJ. Dietary salt, urinary calcium and bone loss. J Bone Miner Res 1996; 11:731-736.

  29. 29.Hiltunen A, Vuorio E, Aro HT. A standardized experimental fracture in the mouse tibia. J Orthop Res 1993;11:305-312.

  30. 30.Landry SP, Marino AA, Sadasivan KK, Albright AJ.Bone injury response. Clin Orthop 1996; 332:260-273.




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