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2005, Number 1

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Med Sur 2005; 12 (1)

The utility of a strategy in detection of serum antibodies against T. pallidum in blood donors

Baptista GH, Jiménez RL, Cedillo VF, Santamaría HC, Santiago MJ, Bordes AJ

Language: Spanish
References: 21
Page: 26-31
PDF size: 61.27 Kb.


ABSTRACT

Objective: To present the results in a specific strategy on detection of T. pallidum in blood donors, with the screening by an immunoenzymatic test. Material and methods: We included consecutively blood donors of Medica Sur Blood Bank and studied specific serum antibodies (IgG and IgM) against the T. pallidum, with an ELISA test that detects the recombinant antigens p15, p17 and p47. They were stratified according relations values of absorbance sample between cut off point (AP/COP). The ELISA reactive cases were analyzed with VDRL and FTA-ABS test. Results: We evaluated 6,850 donors, in 116 cases (1.7%) were reactive to ELISA. With AP/COP ≤ 0.999 (n 29), we not observed reactivity in VDRL or FTA-ABS in 27 cases (proportion 0.92). With values 1.0 to 1,900 (n 52), in 40 cases were without reactivity (0.77), With AP/COP ≥ 2.0 (n 37), always were reactivity to FTA-ABS (0.06), VDRL (0.08) or both tests (0.86). The critical value of the AP/COP by analysis ROC was 0.915 (CI 95% 0.870-0.961). Thus when comparing itself with the AP/COP, the FTA-ABS it shows sensitivity of 91.0% (CI 95% 84.2-97.9), specificity of 97.4% (CI 95% 93.8-100). The sensitivity of VDRL test was 81.4% (CI 95% 69.8-93.0). With both reactive tests (VDRL and FTA-ABS) show sensitivity of 43.3% (CI 95% 31.4-55.1) and specificity of 100%. Conclusions: The greater sensitivity and specificity of immunoenzymatic test. along with the fact of being a simple, objective, and automated test, turn it the technique of election to study of blood donors.


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