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2005, Number 1

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Bioquimia 2005; 30 (1)

Isolation of Aeromonas producers of aerolysins and enterotoxins, from the municipal drinking water system at Facultad de Estudios Superiores Zaragoza and other dependences of the Universidad Nacional Autónoma de México

Tequianes-Bravo L, Pérez-González DA, González MMM, Flores-Pimentel M, Marroquín-Segura R

Language: Spanish
References: 29
Page: 23-29
PDF size: 70.54 Kb.


ABSTRACT

The present work had as purpose to isolate and to characterize mesophilic members of the genus Aeromonas in 70 samples from municipal drinking water system supplies at Facultad de Estudios Superiores Zaragoza (FES) and other dependences of the Universidad Nacional Autónoma de México (UNAM). Aeromonas strains isolated were characterized biochemically and looking for the presence of extracellular products. In the results, thirty seven strains of the genus Aeromonas were isolated, that represented 58.8% of the sampling realized. The percentages isolations of species of Aeromonas were: A. hydrophila 45.9%, A. sobria 18.9%, A. caviae 16.2%, A. jandaei 13.5%, A. salmonicida 2.7%, and A. veronii 2.7%. Aerolysin activity was reported in units of hemolysins causing 50% hemolysis to rat erythrocytes, media values of these units in each strain were: A. hydrophila 366.5 ± 48.0, A. caviae 334.3 ±114.5, and A. sobria 261.9 ± 42.1. The enterotoxin production was quantified by the amount of liquid infiltrated in segments of rabbit ileon, and it was higher in A. hydrophila 3.25 ± 0.58 and A. sobria 2.65 ± 1.35 mL. As conclusion, the high percentage of A. hydrophila and the presence of virulence factors in these strains, suggest that municipal drinking water system with these microorganisms have potential risk to produce infections in humans.


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