2008, Number 4
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salud publica mex 2008; 50 (4)
Molecular epidemiology of cattle and human tuberculosis in Mexico
Pérez-Guerrero L, Milián-Suazo F, Arriaga-Díaz C, Romero-Torres C, Escartín-Chávez M
Language: Spanish
References: 22
Page: 286-291
PDF size: 282.37 Kb.
ABSTRACT
Objective. The purpose of the study was to determine the role of bovine TB in cases of human TB.
Material and Methods. Two-hundred and fifty-five samples from symptomatic patients were included in the study. All samples were cultured in Stonebrink and Lowënstein-Jensen media and analyzed using a nested PCRMPB70. The molecular analysis was performed by spoligotyping.
Results. From 255 samples, 74 were PCR-positive and 20 were culture-positive. From 94 samples positive to PCR or to isolation, 66 (70%) showed a spoligotype compatible with
M. tuberculosis, and 13 (13.8%) with
M. bovis. Four fingerprints of
M. bovis from humans were identical to the fingerprints of
M. bovis from cattle in the same region.
Conclusions. Our study shows that
M. bovis plays an important role in the epidemiology of TB in humans and that TB in cattle represents a risk to public health.
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