medigraphic.com
Instituto Nacional de Salud Pública

2007, Number 1

<< Back Next >>

salud publica mex 2007; 49 (1)

Trypanosoma cruzi in triatomines from Nuevo Leon, Mexico.

Molina-Garza ZJ, Rosales-Encina JL, Galaviz-Silva L, Molina-Garza D

Language: Spanish
References: 27
Page: 37-44
PDF size: 172.52 Kb.


ABSTRACT

Objective. To determine the prevalence of Trypanosoma cruzi in triatomines from Nuevo León using the standardization of an improved enzyme-linked immunosorbent assay test. Materials and Methods. From July to September 2005, 52 triatomines were captured in General Terán, a municipality located in Nuevo León. They were analyzed using optical microscopy (OM) and a polymerase chain reaction (PCR), as standards of reference, to develop a technique for detecting the parasite using enzyme-linked immunosorbent assay (ELISA). Results. Using OM and PCR, 31 triatomines were found to be positive and 21 negative. Using ELISA, 27 samples were identified as positive and 25 negative (specificity 100%, sensitivity 87%, negative predictive value 84%, and positive predictive value 100%). The prevalence of infected triatomines was 59.61% with OM and PCR, and 51.92% with ELISA. Our data confirm that the ELISA assay in triatomines is a fast, reliable and useful tool. Conclusions. Since it was possible to simultaneously analyze a large number of samples with high sensibility and specificity values, the ELISA test proves to be useful for new epidemiologic studies having a high number of vectors. It is also less expensive than PCR. It is therefore recommended for epidemiological and preventive surveillance programs as a first screening test before conducting a confirmatory test using PCR.


REFERENCES

  1. Benenson AS. El control de enfermedades transmisibles en el hombre: Informe oficial de la Asociación Estadounidense de Salud Pública. 15a. edición. Pub Cient 1992;538.

  2. Duthie MS, Kahn M, White M, Kapur RP, Kahn SJ. Critical proinflammatory and anti-inflammatory functions of different subsets of CD1d-restricted natural killer T cells during Trypanosoma cruzi infection. Infect Immun 2005;73:181-192.

  3. Pinto-Días JC. Epidemiology of Chagas’ disease. En: Wendel S, Brener Z, Camargo ME, eds. Chagas’ disease (American Tripanosomiasis): Its impact on transfusions and clinical medicine. A ISBT Brazil’92 1992:49-80.

  4. Vidal-Acosta V, Ibáñez-Bernal S, Martinez-Campos C. Natural Trypanosoma cruzi infection of triatominae bugs associated with human habitations in Mexico. Salud Pública Mex 2000;42:496-503.

  5. Martínez-Ibarra A, Galaviz-Silva L, Trujilla C. Distribución de los triatominos asociados al domicilio humano en el municipio de General Terán, Nuevo León, México. Southwestern Entomol 1992;17:261-265.

  6. Aguirre-Pequeño E. Una nueva distribución geográfica de los triatomas naturalmente infectados por Trypanosoma cruzi en la República mexicana. Arch Med Mex 1947;8:350-358.

  7. Galaviz-Silva L, Ramírez E, Vázquez V. Histotropismo y patogenicidad de Trypanosoma cruzi en ratón albino (NHI) aislado de triatominos en Nuevo León, México. Bol Chil Parasitol 1992;47:3-10.

  8. Galavíz-Silva L, Arredondo JM, Ramírez V. House Triatomineae at the San Juan de Vaquerías Comunal Land (“Ejido”) in Nuevo León, México. J Border Health 1991;7:16-25.

  9. Instituto Nacional de Estadística, Geografía e Informática. Disponible en://www.inegi.gob.mx/est/contenidos/espanol/proyectos/censos/ce2004 [consultado el 22 de noviembre, 2005].

  10. Sambrook J, Russell DW. Molecular Cloning. A laboratory Manual. 3rd edition. Nueva York: Cold Spring Harbor Laboratory Press, 2001; vol.3:18.67-18.68.

  11. Maizels RM, Blaxter ML, Robertson BD, Selkirk ME. Parasite antigens, parasite genes. A laboratory manual for molecular parasitology. Cambridge: University Press, 1988.

  12. Monteón-Padilla VM, Reyes PA, Rosales JL. Detección de Trypanosoma cruzi en muestras experimentales por el método de reacción en cadena de la ADN polimerasa. Arch Inst Cardiol 1994;64:135-143.

  13. Sosa-Jurado F, Mazariego-Aranda M, Hernández-Becerril N, Garza- Murillo V, Cárdenas M, Reyes PA, et al. Electrocardiographic findings in Mexican chagasic subjects living in high and low endemic regions of Trypanosoma cruzi infection. Mem Inst. Oswaldo Cruz 2003;98:605-610.

  14. Fleiss JL. Statistical methods for rates and proportions. 2a. edition. New Cork: John Wiley and Sons, 1981.

  15. Thrusfield M. Veterinary Epidemiology. 2a. edition. UK: Blackwell Science Ltd, 1995:22-179.

  16. Altman DG. Practical statistics form medical research. Londres: Chapman and Hall, 1991.

  17. Goldsmith RS, Zarate RJ, Zárate LG, Kagan I, Jacobson LB, Morales G. Estudios clínicos y epidemiológicos de la enfermedad de Chagas en Oaxaca, México, y un estudio complementario de siete años, Cerro del aire. Bol of Sanit Panam 1986;100:145-166.

  18. Becerril-Flores M, Valle-de la Cruz A. Descripción de la enfermedad de Chagas en el valle de Iguala, Guerrero, México. Gac Med Mex 2003; 139:539-544.

  19. Franco da Silveira J, Umezawa ES, Luquetti AO. Chagas disease: recombinant Trypanosoma cruzi antigens for serological diagnosis. Trends Parasitol 2001;17:286-290.

  20. Gruber A, Zingales B. Trypanosoma cruzi: characterization of two recombinant antigens with potential application in the diagnosis of Chagas’ disease. Exp Parasitol 1993;76:1-12.

  21. James MB, Shreffler WG, Rosman DE, Sleath PR, March CJ, Reed SG. Identification and synthesis of a major conserved antigenic epitope of T cruzi. Proc Natl Acad Sci 1992;9:1239-1243.

  22. Paranhos-Bacalla GS, Santos MR, Cotrim PC, Rassi A, Jolivet ME, Camargo M, et al. Detection of antibodies in sera from Chagas´ patients using a Trypanosoma cruzi immunodominant recombinant antigen. Parasit Immunol 1994;16:165-169.

  23. Zicker F, Smith PG, Luquetti A, Oliveira OS. Detección de Infectados por Trypanosoma cruzi mediante inmunofluorescencia, ELISA y Hemaglutinación en suero y eluidos de sangre seca. Bol of Sanit 1991; 110:489-497.

  24. Breniere SF, Obsceno MF, Telleria J, Carrasco R, Vargas F, Yasik N, et al. Field application of polymerase chaín reaction diagnosis and strain typing of Trypanosoma cruzi in Bolivian triatomines. Am J Trop Med Hyg 1995;53:179-184.

  25. Lages-Silva E, Ramírez LE, Pedroza AL, Crema E, da Cunha LM, Junho Pena SD, et al. Variability of kinetoplast DNA gene signature of Trypanosoma cruzi II strains from patients with different clinical forms of Chagas´ disease in Brazil. J Clin Microbiol 2006;44:2167-2171.

  26. Segura EL, Escobar-Mesa A. Epidemiología de la enfermedad de Chagas en el estado de Veracruz. Salud Pública Mex 2005;47:201-208.

  27. Zavala-Castro J, Velasco O, Hernández R. Molecular characterization of mexican socks of Trypanosoma cruzi using total DNA. Am J Trop Med Hyg 1992;47:201-209.




2020     |     www.medigraphic.com