Evaluation of probiotic properties in Lactobacillus isolated from small intestine of piglets

Iñiguez-Palomares C; Pérez-Morales R; Acedo-Félix E

2007, Number 3-4

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Microbiología 2007; 49 (3-4)

**Evaluation of probiotic properties in Lactobacillus isolated from small intestine of piglets**

Iñiguez-Palomares C, Pérez-Morales R, Acedo-Félix E

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Language: English
References: 50
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ABSTRACT

Lactobacillus has been associated with beneficial effects in human and animal health. Oral administration of probiotic bacteria must resist gastrointestinal transit, in order to colonize the intestinal mucus and offer an antagonistic effect against pathogenic microorganisms. The aim of this work was to select Lactobacillus strains isolated from small intestine of piglets based on the characteristics of resistance to low pH and bile salts, surface properties and antagonistic effect against Escherichia coli K88. To identify Lactobacillus species, a fragment of 16S rRNA gene of the strains was sequenced. Low pH, bile salts resistance and antagonistic activity were quantified by viable count in plates. Surface properties were measured using a spectrophotometer at 600 nm. Sixty-two Lactobacillus strains were isolated from small intestine of piglets. Species Lactobacillus salivarius, Lactobacillus reuteri and Lactobacillus mucosae were identified. We found that 20 Lactobacillus strains resisted low pH and bile salt, 8 of them were adherents and they inhibited in vitro the growth of E. coli K88. In conclusion, our results showed that 8 strains have potential probiotic value, according resistance to gastrointestinal tract, surface properties and antagonistic characteristics. Lb. salivarius was the species that fulfilled the criterion to be identified as a possible probiotic microorganism.

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