2007, Número 3-4
<< Anterior Siguiente >>
Microbiología 2007; 49 (3-4)
Evaluation of probiotic properties in Lactobacillus isolated from small intestine of piglets
Iñiguez-Palomares C, Pérez-Morales R, Acedo-Félix E
Idioma: Ingles.
Referencias bibliográficas: 50
Paginas: 46-54
Archivo PDF: 183.13 Kb.
RESUMEN
Con frecuencia los lactobacilos se han relacionado con diversos efectos benéficos en la salud humana y animal. En la administración oral de bacterias benéficas, éstas deben resistir el tránsito gastrointestinal para que colonicen la mucosa y ejerzan un efecto antagónico contra diversas bacterias patógenas. Por lo anterior el objetivo de este trabajo fue seleccionar cepas de
Lactobacillus aisladas del intestino delgado de lechones de acuerdo a sus características de resistencia al pH bajo y sales biliares, propiedades de superficie y efecto antagónico en contra de
Escherichia coli K88 para utilizarlas a futuro como probióticos. Se aislaron sesenta y dos cepas de
Lactobacillus del intestino delgado de seis lechones. Se identificaron las especies
Lactobacillus salivarius, Lactobacillus reuteri y
Lactobacillus mucosae. Encontramos que 20 cepas de
Lactobacillus resistieron la presencia de pH bajo y sales biliares, 8 de ellas fueron adherentes e inhibieron
in vitro el crecimiento de
E. coli K88. En conclusión, nuestros resultados mostraron que 8 cepas tienen valor probiótico potencial de acuerdo a la resistencia al tránsito gastrointestinal, propiedades de superficie y efecto antagónico.
Lb. salivarius fue la especie que cumple con los criterios para ser identificada como un probiótico potencial para aplicarse en cerdos recién destetados.
REFERENCIAS (EN ESTE ARTÍCULO)
Altschul S., T. Madden, A. Shäffer, J. Zhang, Z. Zhang, W. Miller & D. Lipman. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res 25:3389-3402.
Bibiloni, R., P.F. Perez, & G.L. De Antoni. 1999. Factors envolved in adhesion of bifidobacterial strains to epithelial cells in culture. Anaerobe 5:483-485.
Bibiloni, R., P.F. Pérez,, L.G. Garrote, E.A. Disalvo & G.L De Antoni . 2001. Surface characterization and adhesive properties of Bifidobacteria. Methods Enzymol 336:411-427.
Casey, P.G., G.E. Gardiner, G. Casey, B. Bradshaw, P.G. Lawlor, P.B. Lynch, F.C. Leonard, C. Stanton, R.P. Ross, G.F. Fitzgerald & C. Hill. 2007. A five-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged with Salmonella enterica Serovar Typhimurium. Appl Environ Microbiol 73:1858-1863.
Chung, H., Y.B. Kim, S.L. Chun, & G.E. Ji. 1999. Screening and selection of acid and bile resistant bifidobacteria. Int J Food Microbiol 47:25-32.
Clark, P.A. & J.H. Martin. 1994. Selection of bifidobacteria for use as dietary adjunts in cultured dairy foods: III – Tolerance to simulated bile concentrations of human small intestine. Cult Dairy Prod J 29:18-21.
Corona, V. 2001. Aislamiento e identificación de Bifidobacterium sp. a partir del contenido gástrico del cerdo. Thesis. University of Sonora. Sonora, Mexico.
Corona, V. 2003. Evaluación probiótica de especies de Bifidobacterium en cerdos lactantes. Thesis. Research Center in Food and Development. Sonora, Mexico.
De Angelis M., S. Siragusa, M. Berloco, L. Caputo, L. Settanni, G. Alfonsi, M. Amerio, A. Grandi, A. Ragni & M. Gobetti. 2006. Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding. Research Microbiol 157:792-801.
De los Reyes C., G. Limsowtin, P. Tailliez, L. Séchaud & J. Accolas J. 1992. A Lactobacillus helveticus-specific DNA probe detects restriction fragment length polymorphisms. Appl Environm Microbiol 58:3429-3432.
De Martinis, E., V. Alves & B. Franco. 2002. Fundamentals and perspectives for the use of bacteriocins produced by lactic acid bacteria in meat products. Food Reviews Int 18:191-208.
Del Re, B., A. Busetto, G. Vignola,, B. Sgorbati & D.L. Palenzona. 1998. Autoaggregation and adhesion ability in a Bifidobacterium suis strain. Lett Appl Microbiol 27:307-310.
Del Re, B., B. Sgorbati, M. Miglioli & D. Palenzona. 2000. Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett Appl Microbiol 31:438-442.
Doyle, M.E. 2001. Alternatives to antibiotic use for growth promotion in animal husbrandy. Food Research Institute April 2001.
Fuller, R. 1999. Probiotics for farm animals. pp. 15-43. In Tannok G.W. Probiotics: A Critical Review. Horizon Scientific Press. Wymondham, U.K.
García-Galaz, A., R. Perez-Morales, M.E. Díaz-Cinco & E. Acedo-Félix. 2004. Resistance of Enterococcus strains isolated from pigs to gastrointestinal tract and antagonistic effect against E. coli K88. Rev Latinoam Microbiol 46:5-11.
Gibson, G.R. & X. Wang. 1994. Regulatory effects of bifidobacteria on the growth of other colonic bacteria. J Appl Bacteriol 77:412-420.
González, S.N., M.C. Apella, N.C. Romero, M.E. Nader de Macías & G. Oliver. 1993. Inhibition of enteropathogens by Lactobacilli strains used in fermented milk. J Food Prot 56:773-776.
Gómez-Gil, B., A. Roque, J. Turnbull & V. Inglis. 1998. A review on the use of microorganisms as probiotics. Rev Latinoam Microbiol 40:166-172.
Gómez-Zavaglia, A., G. Kociubinski, P. Pérez & G. De Antoni, 1998. Isolation and characterization of Bifidobacterium strains for probiotic formulation. J Food Prot 61:865-873.
Gómez-Zavaglia, A., G. Kociubinski, P. Pérez, E. Di Salvo & G. De Antoni. 2002. Effect of bile on the lipid composition and surface properties of bifidobacteria. J Appl Microbiol 93:794-799.
Gopal K.P., P. Prasad, J. Smart & H. Gill. 2001. In vitro adherence of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli. Int J Food Microbiol 67:207-216.
Grill, J.P., S. Perrin & F. Schneider. 2000. Bile salt toxicity to some bifidobacteria strains: role of conjugated bile salt hydrolase and pH. Can J Microbiol 46:878-884.
Gusils, C., M. Bujazha & S. González. 2002. Preliminary studies to design a probiotic for use in swine feed. Interciencia 27:409-413.
Hernández, F. 2003. Algunos aspectos de investigación básica y aplicada sobre probióticos. Memoria electrónica del 3er Simposio Mexicano de Probióticos. México D.F.
Hintze, J.L. 1997. NCSS 97 user’s guide-II [Computer software manual] Kaysville, UT: Number Cruncher Statistical Systems.
Ibrahim, S. & A. Bezkorovainy. 1993. Survival of bifidobacteria in the presence of bile salt. J Animal Sci 62:351-354.
Jonson, E. & P. Conway. 1992. Probiotics for pigs, pp. 84-93. In Fuller R. (ed). Probiotics. The Scientific Basis. Chapman and Hall editorial. London, UK.
Kandler O. & N. Weiss. 1986. Genus Lactobacillus Beijerinck 1901, pp. 1209-1234. In P.H.A. Sneath, N.S. Mair, M.E. Sharpe & J.G. Holts (eds). Bergey´s Manual of Systematic Bacteriology. vol. 2. Williams & Wilkins Co. Baltimore, Md.
Kim, G.B., S.H. Yi & B.H. 2004. Purification and characterization of three different types of bile salt hydrolases from Bifidobacterium strains. J Dairy Sci 87:258-266.
Kociubinski, G., P.F. Perez, M.C. Añon & G.L. De Antoni. 1999. A method for the screening of lactic acid bacteria with high inhibitory power. J Food Prot 59:739.
Lane D.J. 1996. 16S/23S rRNA sequencing, pp. 115-147 In Stackebrandt E. & M. Goodfellow (Eds). Nucleic Acid Techniques In Bacterial Systematics. John Wiley and Sons Chichester, England.
Matsumoto, M., H. Oishi & Y. Benno. 2004. H+ ATPase activity in Bifidobacterium with special reference to acid tolerance. Int J Food Microbiol 93:109-113.
Maxwell F.J. & C.S. Stewart 1995. The Microbiology of the Gut and the Role of Probiotics, pp. 155-177. In Varley M.A (ed). The neonatal Pig Development and Survival. Ed. Cab International. Leeds, UK.
Meng, Q., M.S. Kerley, T.J. Russel & G.L. Allee. 1998. Lectin-like activity of Escherichia coli K88, Salmonella choleraesuis and Bifidobacterium pseudolongum of porcine gastrointestinal origin. J Anim Sci 76:551-556.
Mishra V. & D. Prasad. 2005. Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int J Food Microbiol 103:109-115.
Mulder, R.W.A.W., R. Havenaar & H. Huis in ´t Veld. 1997. Intervention strategies: the use of probiotics and competitive exclusion microflora against contamination with pathogens in pigs and poultry, pp. 187-205. In Fuller R (ed). Probiotics 2: Application and Practical Aspects. Chapman & Hall. London, UK.
Ofek, I. & R.J. Doyle. 1994. Bacterial Adhesion to Cells and Tissues, pp. 80-84. Chapman & Hall. New York.
Ouwehand, A.C., P.V. Kirjavainen, S. Colette & S. Salminen. 1999. Probiotics: Mechanisms and established effects. Int Dairy J 9:43-52.
Pérez, P. F., Y. Minnaard, A. Disalvo, A & G.L. De Antoni. 1998. Surface properties of bifidobacterial strains of human origin. Appl Environ Microbiol 64:21-26.
Rinkinen, M., E. Westermarck, S. Salminen & A. Ouwehand. 2003. Absence of host specificity for in vitro adhesion of probiotic lactic acid bacteria to intestinal mucus. Vet Microbiol 97:55-61.
Robredo B. & C. Torres. 2000. Bacteriocin production by Lactobacillus salivarius of animal origin. J Clin Microbiol 38:3908-3909.
Rodríguez E., J. Arqués, M. Rodríguez & M. Medina. 2003. Reuterin production by lactobacilli isolated from pig faeces and evaluation of probiotic traits. Letters Appl Microbiol 37:259-263.
Roos S., F. Karner, L. Axelsson & H. Jonsson. 2000. Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. Int J System Evol Microbiol 50:251-258.
Saarela M., G. Mogensen, R. Fondén, J. Matto & T.M. Sandholm. 2000. Probiotic bacteria : safety, funtional and technological properties. J Biotech 84:197-215.
Schierack P., L.H. Wieler, D. Taras, V. Herwig, B. Tachu, A. Hlinak, M.F. Schmidt & Scharek L. 2007. Bacillus cereus var. toyoi enhanced systemic immune response in piglets. Vet Immunol Immunopathol 118:1-11.
Shah, H., J. Lee, J. Pestka & Z. Unstund. 1999. Viability of bifidobacteria in commercial dairy products during refrigerated storage. J Food Prot 63:327-331.
Tanaka, H., H. Hashiba & J. Kok. 2000. Bile salt hydrolase of B. longum- biochemical and genetic characterization. Appl Environ Microbiol 66:2502-2512.
Tuomola, E., R. Crittenden, M. Playne, E.I. Solauri & S. Salminen. 2001. Quality assurance criteria for probiotic bacteria. Am J Clin Nutr 73(Suppl):393S-398S.
Yusof, R.M., F. Haque, M. Ismail & Z. Hassan. 2000. Isolation of B. infantis and its antagonistic activity against ETEC O157 and Salmonella typhimurium S-285 in weaning foods. Asia Pacific J Clin Nutr 9:130-135.