2007, Number 4
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Vet Mex 2007; 38 (4)
Prolactin stimulates endocytosis of Staphylococcus aureus in bovine mammary ephithelial cells
Ochoa ZA, Gutiérrez BA, Lara ZL, Loeza LPD, Anaya LJL, López MJE
Language: English/Spanish
References: 20
Page: 455-465
PDF size: 345.06 Kb.
ABSTRACT
Bovine mastitis provokes inflammation of the mammary gland and is mainly caused by
Staphylococcus aureus. Highest incidence occurs at drying off period and parturition, where prolactin (PRL) has a relevant function, although its role in the infection has not been studied. In this work,
in vitro effect of the bovine PRL (1-50 ng/mL) on endocytosis and intracellular proliferation of
S. aureus (ATCC 27543) in bovine mammary epithelial cells (BMEC) was evaluated. The BMEC treated during 24 h with 5 ng/mL of bPRL increases ~3 fold the endocytosis of
S. aureus, besides inducing bacterial proliferation. This effect was specifically reverted by treating the BMEC with IgG anti-bPRL.
S. aureus increased (~3 fold) its endocytosis in BMEC treated with bPRL (5 ng/mL), but the number of infected cells was not modified. In conclusion, the bPRL stimulates the endocytosis and the proliferation of
S. aureus in BMEC.
REFERENCES
Wanasinghe DD. Adherence as a prerequisite for infection of the bovine mammary gland by bacteria. Acta Vet Scand 1981; 22: 109-117.
Burton JL, Erskine RJ. Immunity and mastitis. Some new ideas for an old disease. Vet Clin Food Anim 2003; 19: 1-45.
Burvenich C, Paape MJ, Hoeben D, Dosogne H, Massart-Leën AM, Blum J. Modulation of the infl ammatory reaction and neutrophil defense of the bovine lactating mammary gland by growth hormone. Dom Anim Endocrinol 1999; 17: 149-159.
Ochoa-Zarzosa A. La prolactina: una hormona con diversidad funcional. Ciencia Nicolaita 2003; 35: 115-128.
Svennersten-Sjaunja K, Olsson K. Endocrinology of milk production. Dom Anim Endocrinol 2005; 29: 241-258.
Neville MC, McFadden TB, Forsyth I. Hormonal regulation of mammary differentiation and milk secretion. J Mammary Gland Biol Neoplasia 2002; 7: 49-66.
Feola RP, Collins MT, Czuprynski CJ. Hormonal modulation of phagocytosis and intracellular growth of Mycobacterium avium ss. paratuberculosis in bovine peripheral blood monocytes. Microb Pathogen 1999; 26: 1-11.
Benedetto N, Folgore A, Romano-Carratelli C, Galdiero F. Effects of cytokines and prolactin on the replication of Toxoplasma gondii in murine microglia. Eur Cyt Net 2001; 12: 348-358.
Kerro-Dego O, van Dijk JE, Nederbragt H. Factors involved in the early pathogenesis of bovine Staphylococcus aureus mastitis with emphasis on bacterial adhesion and invasion. A review. Vet Q 2002; 24: 181-198.
Hockett ME, Hopkins FM, Lewis MJ, Saxton AM, Dowlen HH, Oliver SP et al. Endocrine profi les of dairy cows following experimentally induced clinical mastitis during early lactation. Anim Reprod Sci 2000; 58: 241-251.
Clevenger CV, Plank TL. Prolactin as an autocrine/ paracrine factor in breast tissue. J Mammary Gland Biol Neoplasia 1997; 2: 59-68.
Anaya-Lopez JL, Contreras-Guzman OE, Carabez-Trejo A, Baizabal-Aguirre VM, Lopez-Meza JE, Valdez-Alarcon JJ et al. Invasive potential of bacterial isolates associated with subclinical bovine mastitis. Res Vet Sci 2006; 81: 358-361.
Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory NY: Cold Spring Harbor, 2001.
Baglia AL, Cruz D, Shaw JE. An Epstein-Barr virus negative burkitt lymphoma cell line (sfRamos) secretes a prolactin-like protein during continuous growth in serum-free medium. Endocrinology 1991; 128: 2266-2272.
Tanaka T, Shiu R, Gout P, Beere C, Noble RL, Friesen JA. A new sensitive and specific bioassay for lactogenic hormones: measurement of prolactin and growth hormone in human serum. Clin Endocrin Metab 1980; 51: 1058-1063.
Anaya-Lopez JL, Lopez-Meza JE, Baizabal-Aguirre VM, Cano-Camacho H, Ochoa-Zarzosa A. Fungicidal and cytotoxic activity of a Capsicum chinense defensin expressed by endothelial cells. Biotechnol Lett 2006; 28: 1101-1108.
Fuh G, Colosi P, Woods WI, Wells JA. Mechanism-base design of prolactin receptor antagonists. J Biol Chem 1993; 268: 5376-5381.
Hauck C, Ohlsen K. Sticky connections: extracellular matrix protein recognition and integrin-mediated cellular invasion by Staphylococcus aureus. Curr Opin Microbiol 2006; 9: 5–11.
Zoubiane GS, Valentijn A, Lowe ET, Akhtar N, Bagley S, Gilmore AP et al. A role for the cytoskeleton in prolactin-dependent mammary epithelial cell differentiation. J Cell Sci 2004; 117: 271-280.
Bayles KW, Wesson CA, Liou LE, Fox LK, Bohach GA, Trumble WR. Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells. Infect Immun 1998; 66: 336-342.
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