Ochoa ZA, Gutiérrez BA, Lara ZL, Loeza LPD, Anaya LJL, López MJE

Idioma: Español/Inglés
Referencias bibliográficas: 20
Paginas: 455-465
Archivo PDF: 345.06 Kb.

RESUMEN

La mastitis bovina provoca la inflamación de la glándula mamaria y es ocasionada principalmente por Staphylococcus aureus. Presenta una incidencia mayor durante el parto o después de la lactancia, periodos en que la prolactina (PRL) tiene función relevante, aunque se desconoce su participación en la infección. En este trabajo se evaluó in vitro, el efecto de la PRL bovina (1-50 ng/mL) sobre la endocitosis y proliferación intracelular de S. aureus (ATCC 27543) en células epiteliales mamarias bovinas (CEMB). Las CEMB tratadas durante 24 h con 5 ng/mL de bPRL incrementan ~3 veces la endocitosis de S. aureus, además de inducir la proliferación bacteriana. Este efecto fue revertido de manera específica tratando las CEMB con IgG anti-bPRL. Las CEMB tratadas con bPRL (5 ng/mL) presentaron mayor endocitosis de S. aureus (~3 veces), pero el número de células infectadas no se modificó. En conclusión, la presencia de bPRL favorece la endocitosis y la proliferación de S. aureus en las CEMB.

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