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1998, Number 4

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Vet Mex 1998; 29 (4)

Blood glutathione pool in layer hens fed different natural pigments and sudans

López CM, Rocha HAE, Zentella de PM, Ávila GE, Arrieta AJM

Language: English/Spanish
References: 36
Page: 323-328
PDF size: 556.82 Kb.


ABSTRACT

The aims of this work were to measure the total blood glutathione (TG), reduced glutathione (GSH), and the oxidized glutathione (GSSG) pool; as well as the egg yolk color of 180,59 week old Dekalb Delta Leghorn hens. Birds were randomly assigned to 15 groups of 12 hens each and housed in cages. Experimental treatments by triplicate were as follows: I. no pigment added; II. 9 ppm natural yellow xantophylles (nyx) from the Aztec marigold flower; III. 9 ppm nyx + 4 ppm natural red xantophylles (nrx) from capsicum fruit; IV. 9 ppm nrx + 2 ppm synthetic red xantophylles, also called cantaxantines, and V. 9 ppm nyx + 4 ppm red Sudan. Treatments were fed during 8 weeks. Weekly spectrophotometer blood TG and high performance liquid chromatrography (HPLC) blood GSH and GSSG measurements were performed. Total produced eggs were analyzed for yolk color by the Roche colorimetrical fan and colorimetry reflectancy means. No significant (P›0.05) differences were found in hens' productive performance including: feed intake, egg production and feed conversion norm egg weight. The egg yolk color fan was: 1, 3.8, 10.4, 10.7 and 13.9 for Groups I, III, IV and V, respectively. A significant (P‹0.05) difference was found in group I blood TG (1.19 µmoles/ml) when compared to Groups II,III, IV and V 2:37, 1.95, 2.11 and 2.55 ,µmoles/ml respectively in the first week of the experiment. Blood GSSG was significantly higher in Groups I and IV (10 and 12 nmoles/ml, respectively) when compared to Groups II and III (7 and 9 nmoles/ml, respectively) and highly different to group V (5 nmoles/ml). No differences were found in GSH among the treatments. It is concluded that the main amount of the ingested red Sudan is eliminated through the egg yolk. No clear relationship between the use of different pigments and the changes in blood glutathione levels was found.


REFERENCES

  1. Morales BE, Ávila GE, Shimada MA. Efecto de niveles elevados de vitamina A en dietas de finalización sobre el comportamiento y la pigmentación del pollo de engorda. Téc Pecu Méx 1997;2:87-92.

  2. De Blas BC, Mateos GG. Nutrición y alimentación de gallinas ponedoras. Barcelona, España: Aedos, 1991.

  3. Tirado AFJ. Efecto pigmentante de las xantefilas en el pollo de engorda. Síntesis Avic 1990;3:28-35.

  4. Williams WD. Origin and impact of color of consumer preference for food. Poultry Sci 1992;71:744-746.

  5. Arce MJ, Vázquez PC, López CC, Avila GE. Xantofilas en dietas para pollos de engorda. Síntesis Avic 1990;6:16-18.

  6. Necoechea RR, Márquez ML. Manual de aditivos y suplementos para la producción animal. México (DF): Manual Agrario, 1987.

  7. North MO. Manual de producción avícola. México (DF): El Manual Moderno, 1986.

  8. Ávila GE, Shimada SA, Llamas LG. Anabólicos y aditivos en la producción pecuaria. México (DF): Consultores en Producción Animal, 1990.

  9. Cuca GM, Ávila GE, Pró MA. La alimentación de la aves. Montecillos, Edo. de México: Colegio de Postgraduados, 1990.

  10. Merck Index. An encyclopedia of chemicals, drug and biologicals. 10th ed. Rahway (NJ): Merck & Co. Inc., 1993.

  11. Secretaría de Salud. Ley general de salud. 5a ed. México (DF): Porrúa, 1987.

  12. Jacobs MB. The chemical analysis of food and food products. 3rd ed. Princeton (NJ): Van Nostrand, 1965.

  13. Secretaría de Salud. Reglamento de la Ley Federal de Salud en materia de control sanitario de actividades, establecimientos, productos y servicios. Diario Oficial de la Federación del 18 de enero de 1988.

  14. Krinsky NI, Deneke SM. Interaction of oxygen and oxyradicals with carotenoids. J Natl Cancer Inst 1982;69:205-209.

  15. Marusich WL, Bavernfeind ChJ. Carotenoids as colorants and vitamin A precursors: technological and nutritional applications. New York: Academic Press, 1981.

  16. Peng BL, Nagao A, Terao J, Tanaka K, Suzuki T, Takama K. Antioxidant activity of xantophyll on peroxil radicalmediated phospholipid peroxidation. Acta Biochem Biophys 1992;1126:178-184.

  17. Marchand LL, Yoshizawa CN, Kofonel LN, Hankin JH, Goodman MT. Vegetable consumption and lung cancer risk: a population based case-control study in Hawaii. J Natl Cancer Inst 1990;82:282-284.

  18. Micozzi MS, Beecher GR, Taylor PR, Kachik F. Carotenoids analyses of selected raw and cooked food associated with a lower risk for cancer. J Natl Cancer Inst 1990;82:280-282.

  19. Akerboom TPM. Assay of glutathione disulfide and glutathione mixed disulfide in biological samples. Met Enzymol 1981;77:372-382.

  20. Winkler SB, Orselli MS, Rex ST. The redox couple between glutathione and ascorbic acid: a chemical and physiological perspective. Free Rad Biol Med 1994;17:333-349.

  21. Anderson EM. Glutathione and glutathione delivery compounds. In: Sies H, editor. Advances in pharmacology. Vol 38. San Diego (CA): Academic Press, 1997:65-78.

  22. Williamson WD, Boettcher B, Meisteer A. Intracellular cysteine delivery system that protects against toxicity by promoting glutathione synthesis. Proc Nat Acad Sci 1987;79:6246-6247.

  23. Fujita T, Yamamoto T, Tabata M, Veno T, Fujimoto Y. Defects of reduced glutathione and cysteine on prostaglandine synthesis in rabbit kidney medulla slices. Biochem Physiol 1986;83:29-31.

  24. Sen ChK. Nutritional biochemistry of cellular glutathione. J Nutr Biochem 1997;8:660-672.

  25. Miyasaky S. Effect of chemicals on glutathione peroxidase of chick liver. Res Vet Sci 1991,51:120-122.

  26. Miyazaky S, Motoi Y. Tissue distrihution of monomeric glutathione peroxidase in broiler chicks. Res Vet Sci 1992;53:47-51.

  27. Richie JP, Abraham P, Leutzinger Y. Long-term stability of blood glutathione and cysteine in humans. Clin Chem 1996;42:1100-1105.

  28. National Research Council. Nutrient requirements of poultry. 8th ed. Washington (DC): National Academy Press, 1994.

  29. Asensi M, Sastre J, Pallardó FV, De la Garcia A J, Estrela JM, Vina J. A high performance liquid chromatography method for measurement of oxidized glutathione. Anal Biochem 1994;217:323-338.

  30. Snedecor WG, Cochran GW. Statistical methods. 6th ed. Ames (IO): The Iowa State University Press, 1971.

  31. Enkvetchakul B, Anthony NB, Bottje WG. Liver and blood glutathione in male broiler chickens, turkeys and quail. Poultry Sci 1995;74:885-889.

  32. White CA, Thannickal VJ, Fanburg BL. Glutathione deficiency in human disease. J Nutr Biochem 1994;5:218-226.

  33. Enkvetchakul B, Bottje WG. Influence of diethyl maleate and cysteine on tissue glutathione and growth in broiler chickens. Poultry Sci 1995;74:864-873.

  34. Beutler E. Nutritional and metabolic aspects of glutathione. Ann Rev 1989;9:287-302.

  35. Halliwell B. Oxidants and human disease: some new concepts. FASEB J 1987;1:348.352.

  36. Ziengler DM. Role of reversible oxidation-reduction of enzyme thiols-disulfides in mctabolic regulation. Ann Rev Biochem 1985;54:305-329.




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