1998, Number 2
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Vet Mex 1998; 29 (2)
Detection of Leptospira interrogans antibody in horses used for production of hyperimmune sera
López PM, Ortega SC, Atilano LD, Peña MA
Language: English/Spanish
References: 28
Page: 173-179
PDF size: 506.86 Kb.
ABSTRACT
Sera from 106, one to 21 year old horses coming from the "Instituto Nacional de Higiene" in Mexico, which were used for hiperimmune antisera production, were analized for the microscopic agglutination test (MAT) with 19 serovars of
L. interrogans. Taking into consideration the animal immunological state, sera with titrers 1:400 were examined by the double agar immunodifusion test (DIT) and counterimmunoelectroforesis (CIEF) to discard inespecific reactions. In addition, 5 rabbits were inoculated with the antigens used in horses (rabies virus; tetanic and diphteric toxoids and scorpion and snake poisons), and their sera was tested for the MAT. Eighty eight out of 106 horses (83%) were positive for one or more serovars showing titrers up to 1:6400. Serovars showing more positive reactions were:
autumnalis, australis and
pomona. Serovars with the highest titrers were:
autumnalis,
cynopteri and
pyrogenes (1:6400), and
australis, celledoni, szwajizak and
icterohaemorrhagiae (1:3200). DIT detected one case of total immunological identity among rabies virus and L. pomona (sample 545). CIEF did not detect any precipitation lines. None of the rabbit sera showed titrers against Leptospira.
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