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2004, Number 2

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Bioquimia 2004; 29 (2)

Immunoturbidimetric assay for quantification of human serum IgA, IgG, and IgM

Alonso RV, Melchor RA, López CR, Gómez CI, Zulueta RO, Hernández PL, Álvarez GM, Morales RE, Turró GG

Language: Spanish
References: 25
Page: 45-54
PDF size: 91.20 Kb.


ABSTRACT

The determination of immunoglobulins IgA, IgG and IgM, significantly contribute to the diagnosis of several illnesses caused by immunodeficiencies, monoclonal gammapathy, and chronic and acute infectious diseases among others, what led to the decision of standardizing a method for their quantification using the SUMA Technology. To this aim, the standard, the control serum and the human antisera anti IgA, IgG and IgM were prepared. The immunoglobulin concentrations in the standard were as follows: IgA: 4.75 mg/mL, IgG: 21.5 mg/mL and IgM: 2.61 mg/mL, and they were determined using as reference an secondary standard (Boerhinger Mannheim, Precimat IgA, G, M; Germany). The standards were correlated obtaining the following coefficients: for IgA 0.96; for IgG 0.98; for IgM 0.98, the results of the precision studies (intra and inter assay) were lower than 10%, the recovery values were superior to 96%, and a good parallelism was obtained among the samples assayed. The stability studies of antisera demonstrated that they are reliable after 98 days of having been reconstituted. For the standard a stability of 24 months was obtained, and 12 months for the control serum. The population study results were 2.2± 1.4 mg/mL for IgA, 12.75 ± 3.18 mg/mL for IgG and 1.91 ± 0.84 mg/mL for IgM.


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