Díaz AE, Blasco MJM, Suárez GF

Language: English/Spanish
References: 15
Page: 307-311
PDF size: 42.37 Kb.

ABSTRACT

Sensitivity and specificity of two different concentrations of the card test antigen for the diagnosis of Brucellosis in goats was evaluated. Antigens used were the oficial antigen produced by a national laboratory in Mexico ("Productora Nacional de Biológicos Veterinarios") with a cellular concentration of 8%, and an experimental antigen with a cellular concentration of 3%. Both antigens were made using Brucella abortus strain 1119-3 in a lactate buffer with a pH of 3.6. Sensitivity for the two antigens was determined using 53 sera from Brucella melitensis culture positive goats. Sera from 100 goats, negative to brucellosis and collected in a brucellosis free area were used for specificity determination. Sensitivity for the 8% cellular concentration was 79%. When the concentration was of 3%, sensitivity was 98%. Specificity for both antigens was 100%. It is concluded that the diagnosis of brucellosis in an 8% cellular concentration is not adequate due to its low sensitivity. The use of a 3% cellular concentration in the card test antigen for goats is recommended.

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