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ISSN 0016-3813 (Print)

2007, Number 3

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Gac Med Mex 2007; 143 (3)

Caracterización fenotípica de células epiteliales limbales humanas expandidas in vitro

Luna-Baca GA, Garfias Y, Robles-Contreras A, Jiménez-Martínez MC

Language: Spanish
References: 27
Page: 183-187
PDF size: 529.35 Kb.


ABSTRACT

Introduction: Human limbal epithelial cells (huLEC) have been used for clinical purposes in ocular surface diseases to promote rapid re-epithelisation and restore corneal epithelium integrity. However, in Mexico this technique has not been fully developed. This study was conducted to characterize the huLEC phenotype expanded in vitro using a cell culture technique.
Material and methods: Cells were obtained from limbal tissue, cultured in KSFM medium and analyzed for the expression of vimentin, K, K19, p63, K12, by flow cytometry and immunofluorescence.
Results: The phenotype of cultured cells was vimentin+K+K19+ p63+K12-.
Conclusions: Our results suggest that under these culture conditions huLEC maintained their stem cell phenotype. This culture technique could be used for clinical purposes in Mexico.


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