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ISSN 0016-3813 (Impreso)

2007, Número 3

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Gac Med Mex 2007; 143 (3)


Caracterización fenotípica de células epiteliales limbales humanas expandidas in vitro

Luna-Baca GA, Garfias Y, Robles-Contreras A, Jiménez-Martínez MC

Idioma: Español
Referencias bibliográficas: 27
Paginas: 183-187
Archivo PDF: 529.35 Kb.


RESUMEN

Introducción: Las células epiteliales limbales humanas (CELhu) han sido utilizadas en la clínica para promover la rápida reepitelización en enfermedades de la superficie ocular con la finalidad de restaurar la integridad del epitelio corneal. Sin embargo, esta técnica aún no ha sido desarrollada en nuestro país. El objetivo de este estudio fue desarrollar una técnica de cultivo de CELhu in vitro y caracterizar su fenotipo.
Material y métodos: Las células fueron obtenidas de tejido limbal, cultivadas en medio KSFM y analizadas para la expresión de vimentina, K, K19, p63 y K12 por citometría de flujo e inmunofluorescencia.
Resultados: El fenotipo de las células cultivadas fue vimentina+K+K19+p63+K12-.
Conclusiones: Nuestros resultados sugieren que bajo estas condiciones de cultivo, las CELhu mantuvieron el fenotipo de célula pluripotencial. Esta técnica de cultivo podría ser utilizada con propósitos clínicos en nuestro país.


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