medigraphic.com
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2006, Number 3

<< Back Next >>

Rev Biomed 2006; 17 (3)

Pneumocysti jiroveci in hospital centers of Bolívar State, Venezuela

Cermeño JR, Hernández de CI, Alcalá F, Áppice M

Language: Spanish
References: 29
Page: 169-174
PDF size: 53.33 Kb.


ABSTRACT

Introduction. Pneumocystis jiroveci (P. carinii) pneumonia (NPJ) is an opportunist infection with important morbility and mortality rates in inmunocompromised patients. Epidemiologic characteristics of pneumocystosis in Bolivar state are scarcely known.
Objective. The aim of this study was to assess the prevalence of P. jiroveci in respiratory samples from patients in Bolívar state.
Materials and methods. Forty sputum samples from immunocompromised patients with clinical pneumonia that did not respond to conventional therapy were analyzed. Direct immunofluorescence technique with monoclonal antibodies MERIFLUOR-Pneumocystis® (Meridian Diagnostics, Inc) was used to detect the fungus.
Results. Thirty five percent (n= 14) were positive for P. jiroveci. Age of patients ranged between 15 and 68 years (median: 35.5), 42.8% of patients had Human Immunodeficiency Virus infection, 35.7% chronic neumopathic disease and 14.2% chronic renal insufficiency. Patients showed the following clinical features: cough (100%; n=14), fever (85.7%; n=12), and cephalea (71.4%; n=10).
Conclusions. Those results suggest that P. jiroveci is a microorganism that should be nvestigated in immunocompromised patients with pneumonia who do not respond to conventional antibiotics.


REFERENCES

  1. 1.- Frenkel JK. Pneumocystis jiroveci n sp. From man: morphology physiology and immunology in relation to pathology. Natl Cancer Inst Monograf 1976; 43:13-30.

  2. 2.- Edman JC, Kovacs JA, Masur H, Santi DV, Elwood HJ, Sogin ML. Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the fungi. Nature1988; 334:519-22.

  3. 3.- Stringer SL, Stringer JR, Blaser MA, Walzer PD, Cushion MT. Pneumocystis carinii: sequence from ribosomal RNA implies a close relationship with fungi. Exp Parasitol 1989; 68:450-61.

  4. 4.- Stringer JR, Cushion MT, Wakefield AE. New nomenclature for the genus Pneumocystis. Proceedings of the Seventh International Workshops on Opportunistic Protists. J Eukaryot Microbiol 2001; Suppl:184S-9S.

  5. 5.- Stringer JR, Beard CB, Miller RF, Wakefields AE. A new name (Pneumocystis jiroveci) for Pneumocystis from humans. Emerg Infect Dis 2002; 8:891-6.

  6. 6.- Frenkel JK. Pneumocystis pneumonia, an immunodeficiency-dependent disease (IDD): a critical historical overview. J Eukaryot Microbiol 1999; 46:89S-92S.

  7. 7.- Revised nomenclature for Pneumocystis carinii. Pneumocystis Work-shop. J Eukaryot Microbiol 1994; 121S-2S.

  8. 8.- Wilkin A, Feinberg J. Pneumocystis carinii pneumonia: a clinical review. Am Fam Physician 1999; 60:1699-708.

  9. 9.- Kaplan JE, Sepkowitz K, Masur H, Sirisanthana T, Russo M, Chapman L, et al. Opportunistic infections in persons with HIV or other immunocompromising conditions. Emerg Infect Dis 2001; 7: (3 Suppl):541.

  10. 10.- Anonimo. Direct immunoflorescent detection procedure for Pneumocystis carinii in respiratory tract specimens. Meridian Diagnost 1996; 1-7.

  11. 11.- Bartlett M, Smith J. Pneumocystis carinii, an opportunist in immunocompromised patients. Clin Microbiol Rew 1991; 4:137-49.

  12. 12.- Fernández P, Torres A, Miro J, Villegas C, Mallolas J, Zamora L, et al. Pronostic factors influencing the outcome in Pneumocystis carinii pneumonia in patients with AIDS. Thorax 1995; 50:669-71.

  13. 13.- Garcías-Gill D, Moreno J, Valls E, Vilardell J, Rimola A, Grande L, et al. Neumonía por Pneumocystis carinii en el receptor de transplante. Enferm Infecc Microbiol Clin 1995; 14: 296-9.

  14. 14.- Armbruster C, Hassl A, Kriswanek S. Pneumocystis carinii colonizaction in the absence of immunosuppression. Scand J Infet Dis 1997; 29:591-3.

  15. 15.- Antigues A, Salas A, Amengual I, Serra E. Tiroiditis por Pneumocystis carinii en un paciente con infección HIV. Enferm Infecc Microbiol Clin 1998; 16:207-8.

  16. 16.- García J, García R, López A, Alcaide F. Neumonía por Pneumocystis carinii e infección por el VIH, diagnóstico y tratamiento. Enferm Infecc Microbiol Clin 1998; 16: 36-43.

  17. 17.- Cermeño J, Sandoval M, Saab T, Mohaweche R, Rodríguez C, Mujica D, et al. Neumonía por Pneumocystis carinii en un paciente con linfoma de Hodgkin. Bol. Informativo Las Micosis en Venezuela 1999. Caracas-Venezuela. 33:23-6.

  18. 18.- Panizo M, Reviákina V, Vázquez C. Diagnóstico de Pneumocystis carinii por inmunofluorescencia directa modificada y coloración de Gomori-Grocott. Estudio comparativo. Bol Soc Venez Microbiol 2000; 20:98-103.

  19. 19.- Stager C, Fraire A, Kim H, Estrada R, Davis, J. Grenberg D, Guy ES, et al. Modification of the fungi-fluor and the genetic systems fluorescent antibody methods for detection of Pneomocystis carinii in bronchoalveolar lavage specimens. Arch Pathol Lab Med 1995; 119:142-7.

  20. 20.- Mathis A, Weber R, Kuster H, Spiech R. Simplified sample processing combined with a sensitive one-tube nested PCR assay for detection of Pneumocystis carinii in respiratory specimens. J Med Microbiol 1997; 35:1691-5.

  21. 21.- Ortona E, Margutti P, Tamburrini E, Mencarini P, Visconti E, Zolfo M, et al. Detection of Pneumocystis carini in respiratory specimens by PCR-solution hibridization enzime-linked inmunoassay. J Clin Microbiol 1997; 35: 1589-91.

  22. 22.- Mallolas J, Vall M, Miro J, Gatell J, Xaubet A, Picado C, et al. Diagnóstico de la neumonía por Pneumocystis carinii mediante esputo inducido. Enf Infecc Microbiol Clin 1990; 8:511-3.

  23. 23.- Tuncer S, Ergüven S, Kocagöz S, Ünal S. Comparison of cytochemical staining, immunofluorescence and PCR for diagnosis of Pneumocystis carinii on sputum samples. Scand J Infect Dis 1998; 30:125-8.

  24. 24.- Helweg-Larsen J, Jensen JS, Benfield T, Svendsen UG, Lundgren JD, Lungren B, et al. Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples. J Clin Microbiol 1998; 36: 2068-72.

  25. 25.- Powers C. Diagnosis of infections diseases: a citopathologist's perspective. Clin Microbiol Rew 1998; 11:341-65.

  26. 26.- Villuendas C, Remacha A, Echávarri B, Lezcano A, Omeñaca M, Areazo P, et al. Neumonía diagnostica por broncoscopia en pacientes HIV – Positivos. Enferm Infecc Microbiol Clin 1995; 14:314-6.

  27. 27.- Ittarat I, Asawamahasakda W, Bartlett M, Smith J, Meshnick S. Efects of Atovaquone and other intibitors on Pneumocystis carinii dihydroorotate dehydrogenase. Antimicrob Agents Chemother 1995; 39:325-8.

  28. 28.- Contini C, Villa M, Romani R, Merolla R, Delia S, Ronchetti R, et al. Detectión of Pneumocystis carinii among children with chronic respiratory disorders in the absence of HIV infection and immunodeficiency. J Med Microbiol 1998; 47:329-33.

  29. 29.- Ministerio de Sanidad y Consumo. Plan Nacional sobre el SIDA, España. Recomendaciones del Consejo Asesor Clínico del Plan Nacional sobre el SIDA. 1994. Nº 1. Noviembre 1-5.




2020     |     www.medigraphic.com