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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2006, Number 1

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Rev Biomed 2006; 17 (1)

Micronucleated erythrocytes in preterm newborns in relation to maternal pathology

Batista-González CM, Corona-Rivera JR, Gómez-Meda BC, Zamora-Pérez AL, Ramos-Ibarra ML, Zúńiga-González GM

Language: English
References: 31
Page: 11-16
PDF size: 50.88 Kb.


ABSTRACT

Introduction. The micronucleus assay detects DNA damage. Certain pathologies that are associated with free radical production may increase the frequency of micronucleated erythrocytes (MNE). Here, we have investigated whether pathologies that are associated with the formation of free radicals lead to an increase in embryonic MNE frequencies during pregnancy.
Materials and Methods. We have used the in vivo micronuclei test on peripheral blood erythrocytes to determine MNE frequencies in preterm newborns (PN) from apparently healthy mothers. MNE frequencies were compared with those obtained from the peripheral blood from PN born to mothers with diabetes mellitus (DM), systemic arterial hypertension (SAH) or vaginal infection (VI). Blood samples were grouped according to gender, maternal pathology, and maternal folic acid intake.
Results. In the peripheral blood of PN born to mothers with pathologies the MNE and micronucleated polychromatic erythrocytes (MNPCE) frequencies were higher than in the PN born to mothers without any pathological condition (P‹0.02). In the PN born to mothers with DM both the MNE and MNPCE frequencies were elevated (P‹0.01), whereas in the PN born to mothers with VI only the MNE frequency was elevated (P‹0.03). The MNE frequency in PN whose mothers suffered SAH showed an increase, although the MNE frequency was reduced in female PN and in PN whose mothers took folic acid (P›0.05). The results were evaluated by means of a U-Mann Whitney test.
Discussion. Our results show that the frequency of MNE increased in PN born to mothers with pathologies that involved an increase in free radical production.


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