2007, Number 1
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pendiente 2007; 1 (1)
Prevalence of vaginal infections in symptomatic and asymptomatic pregnant women
Fernando CE, Vay C, Menghi C, Cora EM, Gatta C, Méndez Ó, de Torres R, Ruda VHM, Famiglietti Á, Perazzi B
Language: Spanish
References: 39
Page: 17-22
PDF size: 64.30 Kb.
ABSTRACT
Objective: To study the rates of lower genital tract infections (
Candida spp., bacterial vaginosis (BV),
Trichomonas vaginalis), during pregnancy.
Material and methods: Two hundred and twenty three vaginal swab specimens from pregnant women who attended the Obstetrics Section at the Hospital of Clinics from August 1, 2005 to January 15, 2006, were prospectively and consecutively examined. The diagnosis of
Candida spp. was made by microscopic wet smear examination with saline solution and potassium hydroxide at 10% and culture (Sabouraud agar and blood agar). The diagnosis of BV was made using the Nugent´s score (≥ 7) and the presence of three or more of the following criteria described by Amsel: presence of clue-cells in the microscopic examination, pH ≥ 4.5, fishy odor test positive and thin, homogeneous vaginal discharge. The investigation of
T. vaginalis was made by direct wet mount with saline solution and with SAF/Methylene blue, Giemsa stain, solid medium culture (Modified Columbia agar) and liquid culture media (Modified Thioglycolate Medium).
Results: We documented 22.4% of infections for
Candida spp. by culture, 21.5% for bacterial vaginosis by Nugent´s score and by clinical criteria and 4.5% for
Trichomonas vaginalis by culture, during pregnancy.
Conclusions: The method used in this study diagnosed a rate of infections for
Candida spp., bacterial vaginosis and
Trichomonas vaginalis similar to another authors, during pregnancy. We suggest performing a microbiologic study of vaginal exudates during pregnancy whether clinical symptoms are present or not, in order to install an early and adequate treatment to present possible maternal and perinatal complications.
REFERENCES
Meis PJ, Goldenberg RL, Mercer B, Moawad A, Das A, McNellis D et al. The preterm prediction study: significance of vaginal infections. Am J Obstet Gynecol 1995; 173: 1231-1235.
Blackwell AL, Thomas PD, Wareham K, Emery SJ. Health gains from screening for infection of the lower genital tract in women attending for termination of pregnancy. Lancet 1993; 342: 206-210.
McGregor JA, French JI. Bacterial vaginosis in pregnancy. Obstet Gynecol Surv 2000; 55 (Suppl. 1): S1-19.
Yudin MH. Bacterial vaginosis in pregnancy: diagnosis, screening and management. Clin Perinatol 2005; 32: 617-627.
Martius J, Krohn MA, Hillier SL, Stamm WE, Homes KK. Eschenboch DA. Relationships of vaginal Lactobacillus species, cervical Chlamydia trachomatis and bacterial vaginosis to preterm birth. Obstet Gynecol 1988; 71: 89-95.
The John Hopkins study of cervicitis and adverse pregnancy outcome. Association of Chlamydia trachomatis and Mycoplasma hominis with intrauterine growth retardation and preterm delivery. Am J Epidemiol 1989; 129: 1247-1257.
Mc Donald HM, O´ Loughlin JA, Jolley P, Vigneswaren R, McDonald PJ. Vaginal infection and preterm labor. Br J Obstet Gynecol 1991; 98: 427-435.
Hay PE, Lamont RF, Taylor Robinson D, Morgan DJ, Ison C, Pearson J. Abnormal bacterial colonization of the lower genital tract as marker for subsequent preterm delivery and late miscarriage. Br Med J 1994; 308: 295-298.
Hillier SL, Krohn MA, Cassen E, Easterling TR, Rabe LK Eschenbach DA. The role of bacterial vaginosis and vaginal bacteria in amniotic fluid infection in women in preterm labor with intact fetal membranes. Clin Infect Dis 1995; 20 (Suppl. 2): S276-278.
Purwar M, Ughade S, Bhagat B, Agarwal V, Kulkarni H. Bacterial vaginosis in early pregnancy and adverse pregnancy outcome. J Obstet Gynecol Res 2001; 27: 175-181.
Jacobsson B, Pernevi P, Chidekei L, Platz-Christensen JJ. Bacterial vaginosis in early pregnancy may predispose for preterm birth and postpartum endometritis. Acta Obstet Gynecol Scand 2002; 81: 1006-1010.
De Seta F, Sartore A, Piccoli M, Maso G, Zicari S, Panerari F et al. Bacterial vaginosis and preterm delivery: an open question. J Reprod Med 2005; 50: 313-318.
Subtil D, Denoit V, Le Goueff F, Husson M O, Trivier D, Puech F. The role of bacterial vaginosis in preterm labor and preterm birth: a case-control study. Eur J Obstet Gynceol Reprod Biol 2002; 101: 41-46.
Chaim W, Mazoz M, Wiznitzer A. The prevalence and clinical significance of intraamniotic infection with Candida species in women with preterm labor. Arch Gynecol Obstet 1992; 251: 9-15.
Gibbs RS, Eschenbach DA. Use of antibiotics to prevent preterm birth. Am J Obstet Gynecol 1997; 177: 375-380.
Cotch MF, Pastorek JG, Nugent RP, Hillier SL, Gibbs RS, Martin DH et al. Trichomonas vaginalis associated with low birth weight and preterm delivery. Sex Transm Dis 1997; 24: 353-360.
Calleri L, Porcelli A, Gallello D, Taccani C, Surico N. Vaginosi batterica e rotura prematura delle membrana: open study. Minerva Ginecol 1997; 48: 19-23.
Mazor M, Chaim W, Shinwell ES, Glezerman M. Asymptomatic amniotic fluid invasion with Candida albicans in preterm premature rupture of membranes. Implications for obstetric and neonatal management. Acta Obstet Gynecol Scand 1993; 72: 52-54.
Minkoff H, Grunebaum AN, Schwarz RH, Feldman J. Cummings MC, Clark WL et al. Risk factors for prematurity and premature rupture of membranes: prospective study of the vaginal flora in pregnancy. Am J Obstet Gynecol 1984; 150: 965-972.
Ho CY, Aterman K. Infection of the fetus by Candida in a spontaneous abortion. Am J Obstet Gynecol 1970; 106: 705-710.
Hillier SL, Martis J, Krohn M, Kiviat N, Holmes KK, Eschenbach DA. A case control study of chorioamniotic infection and histologic chorioamnionitis in prematurity. N Engl J Med 1988; 319: 972-978.
Nichols A, Khong TY, Crowther CA. Candida tropicalis chorioamnionitis. Am J Obstet Gynecol 1995; 172: 1045-1047.
Larsson PG, Platz-Christensen JJ, Thejls H, Forsum U, Pahlson C. Incidence of pelvic inflammatory disease after first trimester legal abortion in women with bacterial vaginosis after treatment with metronidazole, a double-blind randomized study. Am J Obstet Gynecol 1992; 166: 100-103.
Cates W, Joesoef MR, Goldman MB. Atypical pelvis inflammatory disease: can we identify clinical predictors? Am J Obstet Gynecol 1993; 169: 341-346.
Whyte RK, Hussain Z, de Sa D. Antenatal infections with Candida species. Arch Dis Child 1982; 57: 528-535.
Germain M, Krohn MA, Hillier SL, Eschenbach DA. Genital flora in pregnancy and its association with intrauterine growth retardation. J Clin Microbiol 1994; 32: 2162-2168.
Costamagna SR. Trichomonas vaginalis. En: Costamagna SR, editors. Parasitosis regionales, un estudio referido a las principales parasitosis de Bahía Blanca, provincia de Buenos Aires, Argentina. Bahía Blanca, Editorial de la Universidad Nacional del Sur, 2004: 197-217.
Poch F, Levin D, Levin S, Dan M. Modified thioglycolate medium: a simple and reliable means for detection of Trichomonas vaginalis. J Clin Microbiol 1996; 34: 2630-2631.
Stary A, Kuchinka-Koch A, Teodorowicz L. Detection of Trichomonas vaginalis on modified agar in the routine laboratory. J Clin Microbiol 2002; 40: 3277-3280.
Nugent RP, Krohn MA, Hillier SL. Reliability of diagnosing bacterial vaginosis is improved by a standarized method of a Gram stain interpretation. J Clin Microbiol 1991; 29: 279-301.
Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, Holmes KK. Nonspecific vaginitis: Diagnosis criteria and microbial and epidemiologic associations. Am J Med 1983; 74: 14-22.
Coppolillo E, Perazzi B, Vay C, Cora Eliseht M, Tauscher P, Barata A y cols. Metodología diagnóstica para las infecciones del tracto genital inferior durante el embarazo. Ginecol Reprod 2000; 7: 76-81.
Klufio CA, Amoa AB., Delamare O, Hombhanje M, Kariwiga G, Igo J. Prevalence of vaginal infections with bacterial vaginosis, Trichomonas vaginalis and Candida albicans among pregnant women at the Port Moresby General Hospital Antenatal Clinic. PNG Med J 1995; 38: 163-171.
Benito-Vilella FJ, Aguilera-Zubizarreta E, Cuesta Perez-Camino A, Pardo VR, Gomez QB, Rodríguez-Patino E et al. Prevalence of vaginal candidiasis in a low-risk obstetric population in Santander. Aten Primaria 2000; 25: 103-106.
Kamara P, Hylton-Kong T, Brothwaite A, Del Rosario GR, Kristensen S, Patrick N y cols. Vaginal infections in pregnant women in Jamaica: prevalence and risk factors. Int J STD AIDS 2000; 11: 516-520.
Shimano S, Nishikawa A, Sonoda T, Kudo R. Analysis of the prevalence of bacterial vaginosis and Chlamydia trachomatis infection in 6,083 pregnant women at a hospital in Otaru, Japan. J Obstet Gynecol Res 2004; 30: 230-236.
Cotch MF, Pastorek JG, Nugent RP, Hillier SL, Gibes RS, Martin DH et al. Trichomonas vaginalis associated with low birth weight and preterm delivery. Sex Transm Dis 1997; 24: 353-360.
Simoes JA, Giraldo PC, Faundes A. Prevalence of cervicovaginal infections during gestation and accuracy of clinical diagnosis. Infect Dis Obstet Gynecol 1998; 6: 129-133.
Di Bartolomeo S, Rodriguez M, Sauka D, de Torres RA. Perfil microbiológico en secreciones genitales en embarazadas sintomáticas en el Gran Buenos Aires, Argentina. Enferm Infecc Microbiol Clin 2001; 19: 99-102.