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Órgano Oficial del Colegio Mexicano de Ortopedia y Traumatología

2000, Number 4

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Acta Ortop Mex 2000; 14 (4)

Osteo-regeneration: coagulite vs hydroxyapatite vs coagulite-hydroxyapatite in experimental maxillary injuries in rats, as determined by computerized tomography and electron microscopy

Aguilar-Cuevas MA, Vega-Zapata R, Abrego-Ayala CE, Junco-Carrera JM, Antuna-Bizarro S, Torán-Sierra J, Novelo GB, García-Garduño MV

Language: Spanish
References: 19
Page: 289-296
PDF size: 114.37 Kb.


ABSTRACT

The purpose of this study was the evaluation of bone regeneration in laboratory produced injuries in the maxilla of Wistar rats, accelerating the process by simple or combinated biomaterials. Coagulite or hidroxyapatite or coagulite-hidroxyhapatite was placed in six Wistar male rats, aged 24 weeks and the following procedures were made according to bioethics rules in experimentation animals: Rats were treated under anesthesia by zolazepam and tiletamin (0.4 ml). Surgical approach by the Semi-Newman technics was performed by four holes in maxilla: two on the right side and two on the left side with 2 mm carbide rounded drill biomaterials were placed in each cavity asfollows: A (coagulite) in zygomaticomaxillary joint of right side; B (hidroxyapatite) between 1st and 2d right molars, C (coagulite-hidroxyapatite) in the left zygomaticomaxillary joint and D (Control) between left 1st and 2nd molars. These lesions were analized by computed axial tomography (CAT) electron microscope scanner and histological analysis. The densitometric values obtained at 3, 6, 9 and 12 weeks were submited to a variant analysis (ANOVA) and demonstrated a significant statistical difference (‹ 0.01). In the surface analysis (filling of cavities) at 3, 6, 9, 12 and 15 weeks, the Kruskal Wallis test was used wih significant statistical difference (p ‹ 0.03). Hystologic analysis, demonstrated coagulite had the best morphodifferentiation process in the micro-structure for tissues engineering. In conclusion CAT is a good alternative for assessment of bone regeneration in vivo and coagulite is better than hidroxyapatite and coagulite-hidroxyapatite in osteoregeneration of experimental maxilla injuries in Wistar rats.


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