2007, Number 2
Reproducibility and variability the control Liquichek urinalysis control (Bio-Rad) Different volumes the evaluation
Villanueva-Jorge S, Salazar-Canul M, Medina-Escobedo M
Full text
Language: Spanish
References: 15
Page: 49-57
PDF size: 155.94 Kb.
ABSTRACT
Despite the advancement of knowledge regarding Quality Control, the Urinalysis area (UQC) is still left behind due to controls’ costs and the existence of some aspects that can affect the results. Manufacturers seldom consider the time in which the control reaches its optimum temperature to be used after storage, the times necessary to invert the sample in order to homogenize it, the minimum quantity required for the control’s optimization, etc. Consequently, evaluating the reproducibility and variability of Liquichek Urinalysis Controls (Bio-Rad) by using 4 mL and 12 mL aliquots of level 1 and level 2, was found to be essential to determine whether working with a standardized procedure and reducing volumes would lower costs of the UQC implementation. Results show that the level 2 of the greater volume maintains reproducibility until the fifth day, whereas the same does not occur with the one of lesser volume, which had greater variation. In addition, although both values remained within the range established by the manufacturer, the 12 mL volume provides more reliability in the results. It is indispensable to remark that the level 2 of the 12 mL volume did not be have within the manufacturer’s specifications, since it indicates a 30-day stability period at 2-8 °C or 10 immersions, and analytes such as ketones, proteins, urobilinogen, and leukocytes were stable until the fourth day of each week.REFERENCES