medigraphic.com

2007, Number 1

<< Back Next >>

Rev Med Hosp Gen Mex 2007; 70 (1)

Staphylococcus epidermidis biofilm-producing in blepharoconjunctivitis

Moreno-González ME, Ruiz-Galindo E

Language: Spanish
References: 39
Page: 24-29
PDF size: 151.09 Kb.


ABSTRACT

Purpose: To compare the presence of Staphylococcus epidermidis biofilm-producing in patients with blepharoconjunctivitis and normal control group. Determined the ability to produce biofilm and antimicrobial susceptibility testing. Method: Prospective cases controls study, S. epidermidis was isolated from eyelid and conjunctival swabs obtained from 47 patients with blepharoconjunctivitis and 46 controls, the presence of biofilm was tested, also the susceptibility to six antibiotics. The results were studied with χ2. Results: S. epidermidis biofilm-producing was found in 70% of patients with blepharoconjunctivitis and 43% without blepharoconjunctivitis (p ‹ 0.05). In patients with blepharoconjunctivitis the degree of biofilm formation was higher. Susceptibility to erythromycin, vancomycin, ciprofloxacin, gatifloxacin, chloramphenicol and oxacyllin do not show differences between S. epidermidis producing and no-producing biofilm. Conclusions: S. epidermidis biofilm-producing was isolated more frequently from patients with blepharoconjunctivitis, in this group the ability to produce biofilm was higher. Susceptibility to six antimicrobials was similar in S. epidermidis producing and no-producing biofilm.


REFERENCES

  1. Rapuano C. Enfermedad de la conjuntiva y las estructuras externas. Los requisitos en Oftalmología. Segmento Anterior. Madrid: Ediciones Harcourt, 2001; 42.

  2. Rees RB Jr. Skin and appendages. In: Krupp MA, Schroeder SA, Tierney LM Jr (eds). Current Medical Diagnosis and Treatment. East Norwalk: CT Appleton and Lange, 1987; 48-49.

  3. Weiss A, Brinser JH, Nazar-Stewart. Acute conjunctivitis in childhood. J Pediatric 1993; 122: 10-14.

  4. Groden LR, Murphy B, Rodnite J et al. Lid flora in blepharitis. Cornea 1991; 10: 50-53.

  5. Au YK, Jensen HG, Rowsey J, Reynolds M. Coagulase negative staphylococci in conjunctivitis and blepharitis. Eye Sci 1993; 9: 129-135.

  6. Taylor PB, Tabbara KF, Burd EM. Effect of preoperative fusidic acid on the normal eyelid and conjunctival bacterial flora. Br J Ophthalmol 1988; 72: 206-209.

  7. Doyle A, Beigi B, Early A et al. Adherence of bacteria to intraocular lenses: A prospective study. Br J Ophthalmol 1995; 79: 347-349.

  8. Singer TR, Isenber SJ. Conjunctival anaerobic and aerobic bacterial flora in pediatric vs adults subjects. Br J Ophthalmol 1988; 72: 448-451.

  9. McNatt J, Allen SD, Wilson LA, Dowell VR. Anaerobic flora of the normal human conjunctival sac. Arch Ophthalmol 1978; 96: 1448-1450.

  10. Cunha M de L, Rugolo ML, Lopes CA. Study of virulence factors in coagulase-negative staphylococci isolated from newborns. Memorias do Instituto Oswaldo Cruz 2006; 101 (6): 661-668.

  11. Bannerman TL, Rhoden DL, McAllister SK, Miller JM, Wilson LA. The source of coagulase-negative staphylococci in the endophthalmitis vitrectomy study. Arch Ophthalmol 1997; 115: 357-361.

  12. O‘Gara JP, Humphreys H. Staphylococcus epidermidis biofilms: Importance and implications. Med Microbiol 2001; 50: 582-587.

  13. Leid JG, Costerton JW, Shirtliff ME, Gilmore MS, Engerbert M. Immunology of staphylococcal biofilm infection in the eye. New tools to study biofilm endophthalmitis. DNA Cell Biol 2002; 21 (5-6): 405-413.

  14. Bainbridge JW et al. Intraocular lens implants and risk of endophthalmitis. Br J Ophthalmol 1998; 82: 1312-1315.

  15. Kodjikian L, Burillon C, Roques C, Pellon G, Freney J, Renaud F. Bacterial adherent of Staphylococcus epidermidis to intraocular lenses: A bioluminescence and scanning electron microscopy study. Invest Ophthalmol Vis Scien 2003; 44: 4388-4394.

  16. Sugita J, Yokoi N, Fullwood N, Quantock A, Takada Y et al. The detection of bacteria and bacterial biofilms in punctal plug holes. Cornea 2001; 20 (4): 362-365.

  17. Henriques M et al. Adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis to silicone-hydrogel contact lenses. Optom Vis Scien 2005; 82 (6): 446-450.

  18. George M, Ahearn D, Pierce G, Gabriel G. Interactions of Pseudomonas aeruginosa and Staphylococcus epidermidis in adhesion to a hydrogel. Eye & Contact Lens: Science & Clinical Practice 2003; 29 (1): S105-109.

  19. Garcia-Saenz MC, Arias-Puente A, Fresnadillo-Martinez MJ, Paredes-Garcia B. Adherence of two strains of Staphylococcus epidermidis to contact lenses. Cornea 2002; 21 (5): 511-515.

  20. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH. Adherence of coagulase-negative Staphylococci to plastic tissue culture plates: A quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol 1985; 22: 996-1006.

  21. Donlan RM, Costerton JW. Biofilms: Survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 2002; 15 (2): 167-193.

  22. Cramton S, Ulrich M, Götz F, Döring G. Anaerobic conditions induce expression of polysaccharide intracellular adhesina in Staphylococcus aureus and Staphylococcus epidermidis. Infect Immun 2001; (69): 4079-4085.

  23. Stickler D. Biofilms. Curr Opin Ophthalmol 1999; 2: 270-275.

  24. Gotz F. Staphylococcus and biofilm. Mol Microbiol 2002; 43: 1367-1378.

  25. Catalanotti P, Lanza M, Del Prete A, Lucido M, Catania MR, Galle F, Boggia D, Perfetto B, Rossano F. Slime-producing Staphylococcus epidermidis an S. aureus in acute bacterial conjunctivitis in soft contact lens wearers. New Microbiologica 2005; 28 (4): 345-354.

  26. Von Eiff C, Peters G, Heilmann C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infect Dis 2002; 2 (11): 285-301.

  27. Costerton JW, Lewandowski Z, Calddwell DE, Korber DR, Lappen-Scott HM. Microbial biofilms. Ann Rev Microbiol 1995; 49: 711-745.

  28. Zegans ME, Becker HI, Budzik J, O´Toole G. The role of bacterial biofilms in ocular infections. DNA Cell Biol 2000; 21 (5-6): 415-420.

  29. Mihara E, Shimizu M, Touge Ch, Inohue Y. Case of a large, movable bacterial concretion with biofilm formation on the ocular surface. Cornea 2004; 23 (5): 513-515.

  30. Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982; 37: 318-326.

  31. Freeman DJ, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol 1989; 42: 872-874.

  32. Tarabishy AB, Hall GS, Procop GW, Jeng BH. Bacterial culture isolates from hospitalized pediatric patients with conjunctivitis. Am J Ophthalmol 2006; 142 (4): 678-680.

  33. Miyanaga Y. A new perspective in ocular infection and the role of antibiotics. Ophthalmologica 1997; 211 (suppl 1): 9-14.

  34. Elder MJ, Stapleton F, Evans E, Dart JK. Biofilm-related infections in ophthalmology. Eye 1995; 9: 102-109.

  35. Yassien M, Khardorin. Interaction between biofilms formed by Staphylococcus epidermidis and quinolones. Diagn Microbiol Infect Dis 2001; 40 (3): 79-89.

  36. El-Azizi M, Rao S, Kanchanapoom T. In vitro activity of vancomycin, quinupristin/dalfopristin linezolid against intact and disrupted biofilms of Staphy. Annu Clin Microbiol 2005; 4: 2-12.

  37. Locatelli CI et al. Conjunctival endogenous microbiota in patients submitted to cataract surgery. Braz J Microbiol 2003; 34 (3): 203-209.

  38. Chisari G, Reibaldi M. Ciprofloxacin as treatment for conjunctivitis. J Chemother 2004; 16 (2): 156-159.

  39. Orden MB, Martinez RR, Millan PR. Bacterial conjunctivitis: Most prevalent pathogens and their antibiotic sensitivity. An Pediatr 2004; 61 (1): 32-36.




2020     |     www.medigraphic.com