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1993, Number 1

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Vet Mex 1993; 24 (1)

Caracterization of Distemper Virus in cell culture

Pérez DS, lturbe RR, Saldívar ZE

Language: English/Spanish
References: 44
Page: 15-19
PDF size: 649.72 Kb.


ABSTRACT

The behavior of Canine Distemper Virus (CDV), in primary cell culture and VERO line cell culture, was evaluated. Different brain isolates of CDV, obtained froni dogs which had died with a neurologic signology, were used. Histopathological and immunofluorescence ( I F ) studies wereperformed. Allbrains showedinclusion bodies andwere positive to the IF reaction. Each sample was inoculated in both VERO line cell culture and in the kidney dog primary cell one. The following paraineters were evaluated: a) cytopathic effect production, b) reaction in the presence of anti-CDV fluorescent conjugate, c) haemoagglutination of red blood cells from different animal species and in different conditions and d)haeinoadsorption to red blood cells. Results according to tlie parameters mentioned above were as follows: a) There were no differences when VERO line cells or priinary cell culture were used. Itwas possible to observe the cytopathic effect in the infected cells ancl no effect in the non-infected cells, b) Antigenicity was not affected in 88% of the samples positive to IF (the greater intensity was observed at 48 hours post-inoculation, c) Only 58% of the samples showed haemoadsorption capacity and d) None of the samples sliowed haeinoagglutination. There were no significant differences between the street viruses and tlie vaccine ones.


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