Pérez DS, lturbe RR, Saldívar ZE

Idioma: Español/Inglés
Referencias bibliográficas: 44
Paginas: 15-19
Archivo PDF: 649.72 Kb.

RESUMEN

Se estudió el comportamiento del virus del Distemper Canino (VDC) en cultivos celulares primarios y de línea. Se emplearon diversos VDC:, aislados de encéfalos provenientes de perros que murieron mostrando iina sintoinatologia nerviosa; histológicamente, dichos encéfalos presentaron cuerpos de inclusión y reacción positiva inmunofluorescencia (IF) al emplear un conjugado anti-DVC. Cada muestra se inoculó en células VER0 y Cultivo Primario de Riñón de perro (CRP) para evaluar la capacidad de: a) producir efectos citopáticos (ECP) , b) reaccionar frente a un conjugado fluorescente anti-DVC, c) hemoaglutinar glóbulos rojos de diversas especies en diferentes condiciones y d) hemoadsorber glóbulos rojos. Los resultados mostraron que: a) no hubo diferencia alguna entre emplear células VERO y CRP, observándose cambios citopáticos que permitieron diferenciar un cultivo infectado de uno no infectado con virus vacunal, b) el 88% de las muestras conservó su antigenicidad, pues resultó positivo en la prueba de IF, observándose la mayor intensidad de fluorescencia especifica a las 48 horas posinfección, c) el 58% de las muestras mostró heinoadsorción y d) ninguna muestra, incluyendo los testigos, fue capaz de hemoaglutinar. No fue posible, con base en los resultados, establecer diferencias entre los virus "de campo" y los vacunales.

REFERENCIAS (EN ESTE ARTÍCULO)

1. Andrewes, C.: Viruses of Vertebrates. 4th ed. Bailhere and Tindall, London, 1978.

2. Axtelm, M., Krakowka, S. and Gorham, J.: Canine Distemper virus: In vivo virulence of in vitro-passaged persistent virus strains. Am..J. vet. Res., 48: 227-233 (1987).

3. Baczko, K.: Restriction of measles virus gene expression in measles indusion body encephahtis..J Znfect. Dis., 158: 144-150 (1988).

4. Behxenkrone, M.: Detection of intracelular canine Distemper virus antigen in mink inoculated with an attenuated or a virulent strain of canine Distemper virus. Am. J. vet. Res., 50: 1616 -1620 (1989).

5. Burge, T., Griot, C., Vandevelde, M. and Peterhans, E.: Antiviral antibodies stimulate production of reactive oxygen species in cultured canine brain cells infected with canine Distemper virus. .f. Virol., 63: 2790-2797 (1989). 6.

6. Calain, P. and Roux, L.: Generation of measles virus defective interfering partides aud their presence in a preparation of attenuated live virus vaccine.J Virol., 62: 2850 -2866 (1988).

7. Carrigan, D.: Rouud cell variant of nieasles virus: Mechanisms involved in the establishment of defective viral infection of the central nervous system. Virology, 155: 61 4 -624 (1986).

8. Chen,T., Goldbaum, M., Wassilak, F.,Morkowitz,E. and Orestein, A.: An explosive point-source measles outbreak in highly vaccinated population. Am.J Epidm., 129: 173 -182 (1989).

9. Cordy, O. and Holiday, A,: Necrotizing meningoencephalitis of Pug dog. Vet. Pathol., 26: 191-194 (1989).

10. Cornwel, H., Thomson, H., McCandlish, A., Macartney, L. and Nash, A.: Encenhalitis in dogs associated with a batch of canine Distemper (Rockborn) vaccine. Vet. Rec., 112: 5459 (1988).

11. Departamento de Patología: Enfermedades diagnosticadas en el Departamento de Patología de la Fac. de Med. Vet. y Zoot., Universidad Nacional Autónoma de México, durante el año de 1974. Vet. Méx., 7: 87-89 (1976). 12.

12. Departamento de Patología: Eufemiedades diagnosticadas en el Departamento de Patología de la Fac. de Med. Vet. y Zoot. de la Universidad Nacional Autónoma de México durante el año de 1976. Vet. Méx., 10: 55-56 (1979).

13. Frankhauser, R. and Vandevelde, M.: Veterinary neurology past, present and future. J. comp. Path., 98: 275-286 (1988).

14. Fenner, F.,Bahcmann, P., Murphy, F., Studdert, M. and Whit, D.: Veterinary Virology. Academic Press, Orlando, Florida, 1987.

15. Freshney, R.: Animal Cell Culture: A Practica1 Approach. IRL, Oxford, England, 1986.

16. Griot, C., Vandevelde, M. and Peterhans, E.: Antibody induced generation of reactive oxygen radicals by brain macrophages in canine Distemper encephalitis: A mechanism for bystander demyelination. Acta Neuropath., 78: 306-403 (1980).

17. Groelke, J., Dixon, L., Cummings, C. and Baseman, J.: Virus contamination and citopathology of Ferret tracheal epitelial cells in culture caused by vaccination with Distemper virus. Lab. Anim. Sci., 35: 527-521) (1986).

18. Howe, C. and Lee, L.:Virus erythrocyte interactions virus advances in research. Vol. XVII. Academic Press, New York, 1972.

19. Iturbe, R.R.: Diagnóstico de moquillo canino por ininunofluorescencia directa en perros diagnosticados clínicamente como rabiosos. Vet. Mex., 20: 161-167 (1989).

20. Johnson, T., Griffin, E. and Moench, R.: Pathogenesis of measles immunodeficiency and encephalomyelitis: Parallels to AIDS. Microb. Path., 4: 169-174 (1988).

21. Kawamura, A.: Fluorescent Antibody Technique and their Applications. 2nd ed. University of Tokyo Press, Tokyo, Japan, 1977. 22.

22. Kimoto, T.: 62 In vitro and in vivo properties of the virus causing natural canine Distemper encephalitis. J. Gen. Virol., 67: 487-503 (1986).

23. Krakowka, S., Cork, L., Winkelstein, J. and Axtelm, M.: Establishment of central nervous system infection by canine Distemper virus: Breach of blood brain barrier and facilitation by antiviral antibody. Vet. Immun. Immunopath., 1 7: 471 -482 (1987).

24. Kuchler, R.: Biochemical Methods in Cell Culture and Virology. Dowdenhutchison, Ross, Pennsylvania, 1977.

25. Leunete, H.: Diagnostic Procedures for: Viral Rickettsial and Clamydial Infections. 5th ed. Am. Pub. Hlth. Ass., Washington, D.C., 1979.

26. McFarland, H. and Dhib, J.: Immune mechanism relating to viral infections of the nervous system. Immun. Alergy Clin. North Am., 8: 223 -238 (1988).

27. McFarland, F. and McFarlin, E.: Humoral and cellular immune responses to matrix protein of measles virus in subacute sclerosing panencephalitis. J. Virol., 62: 2483 -2489 (1988).

28. McLaughlin, B., Adams, P., Cornell, W. and Elkins, D.: Canine Distemper viral inclusions in blood cells of four vaccinated dogs. Can.vet. J., 26: 368 -372 (1985).

29. Meric, S.: Canine meningitis. J. vet. Internal. Med., 2:26-35 (1988).

30. Metzler, E., Krakowka, S., Axthelm, K. and Gorham, R.: In vitro propagation of canine Distemper virus: Establishment of persistent infection in VERO cells. Am..J vet. Res., 45: 2211-2214 (1984).

31. Mirchamsy, H., Shafyi, A., Nazarin, P. and Sassani, A.: Evaluation of hve attenuated measles vaccines prepared in human diploid cells for reimmunization. Epidem. Inf, 101: 437-443 (1988).

32. Morikawa,Y.,Yoshikawa,Y. and Yamanouchi, K.: Characterization of cauine Distemper virus adapted to human neural cells. Microbiol. Immunol, 32: 1211-1220 (1988).

33. Morikawa, Y., Yoshikawa, Y. and Yamanouchi, IC.: Reversion of neurovirulence and in vitro phenotype of neural cell adapted canine Distemper virus after passage in non neural cells. Microbiol. Immunol, 32: 1263 -1268 (1988).

34. Morrison, T.: Structure, function and intracellular processing of paramixovirus membrane proteins. Virus Res., 10: 113 -136 (1988).

35. Olsen, C:. and Krakowka, S.: Inmunología e Inmunopatología de 1os Animales Domésticos. El Manual Moderno, México, D.F., 1083.

36. Porta Della, A,: Veterinary Viral Diseases: Their Significance in South-East Asia and Western Pacific. Academic Press, Orlando, Florida, 1985.

37. Povey, Ch.: Distemper vaccination of dogs: Factors which could cause vaccine failure. Can. vet. J., 27: 321-323 (1986).

38. Rouse,T. and Horohov, W.: Immunosuppresion in viral infections. Rev. Infect. Dis., 8: 223 -238 (1988).

39. Rude, T.: Canine Distemper virus: Infection and prevention. Canine Pract., 14: 16-24 (1987).

40. Shen, D., Cox, N. and Swango, L.: Electrophoretic determination of albumin and gammaglobulin concentrations in the cerebrospinal fluid of dogs with encephalomyelitis attributable to canine Distemper virus infection: 3 cases. J Am. vet. med. Ass., 195: 977-980 (1989).

41. Sheshberadaran, H., Norby, E., Kennet, C:., Carpenter, C. and Orvell, C.: The antigenic relationship between measles, canine Distemper and Rinderpest viruses studied with monoclonal antibodies. J. Gen. Virol, 67: 1381-1391 (1986).

42. Summers, B. and Appel, M.: Syncitia formation: An aid in the diagnosis of canine Distemper encephalomye1itis. J comp. Path, 95: 433 -434 (1985).

43. Thomas, R., Diane, E. and Christine, R.: Acute measles in patients with and with out neurological involvement: Distribution of measles virus antigen and RNA. J. Infect. Dis., 158: 433-442 (1988).

44. Yoshikawa, K., Nomura, Y., Sugiyama, M. and Yamanouchi, K.: Natural infection with canine Distemper virus in Japanese monkey (Macaca fuscata). Vet Microbiol., 20: 193-204 (1989).