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2006, Number 4

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Vet Mex 2006; 37 (4)

Identification of Brucella abortus S19 vaccinal strain in cow milk samples

Martínez CLC, Verdugo RA, Hernández CR

Language: English/Spanish
References: 20
Page: 479-486
PDF size: 258.12 Kb.


ABSTRACT

Brucellosis is an infectious disease caused by bacteria of the genus Brucella which affects several domestic and wild life animals, as well as humans. Until the past decade, strain 19 (S19) of B. abortus was used as the vaccine for brucellosis in cattle in Mexico. Brucella abortus S19 carries a deletion in two genes of the operon ery, responsible for the erythritol catabolic pathway. Since 1997, B. abortus strain RB51 was offi cially approved in Mexico for its use as a vaccine for cattle. This strain has an insertion sequence (IS) named IS711, which interrupts the wboA gen. Based on this knowledge, two PCR assays were standardized to allow the identifi cation of strains S19 and RB51, and distinguish them from other Brucella species and biotypes. These assays were used for characterization of 11 field strains of B. abortus isolated from milk of cattle. A 456-bp DNA fragment was amplifi ed from all strains when PCR, that identifi es RB51, was used, discarding the presence of vaccine strain RB51 among the fi eld strains. In contrast, when the assay to identify strain S19 was performed, a 1063-bp DNA fragment was amplifi ed from nine strains, while the other two were identifi ed as vaccine strain S19 by amplifi cation of a 361-bp DNA fragment. The fi nding of vaccine strains among this isolates is important to both epidemiological and government level, due to their direct and indirect implications on the National Campaign for Control and Eradication of Bovine Brucellosis.


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